Apolipoprotein B (Apo B) - R1032P | acris-antibodies.com

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Product Description for Apolipoprotein B (Apo B)

Goat anti Human Apolipoprotein B (Apo B).
Presentation: Purified
Product is tested for Frozen Sections, Enzyme Immunoassay, Immunoprecipitation, Western blot / Immunoblot, Paraffin Sections.

Properties for Apolipoprotein B (Apo B)

Product Category	Antibodies
Quantity	1 mg
Synonyms	Apo-B, ApoB, ApoB-100, ApoB100, FocusOn036
Presentation	Purified
Reactivity	Hu
Applications	C, E, IP, P, WB
Clonality	Polyclonal
Host	Goat
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	Acris Antibodies GmbH

Datasheet Extract

Immunogen	Swiss Prot Num: P04114 Immunogen: apoLipoprotein Type B isolated from Human plasma by density gradient centrifugation followed by HPLC purification. Genename: 338
Antibody type	Ab
Application	Anti-apoLipoprotein antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for Immunoprecipitation and for Western blotting for highly sensitive analysis. Recommended Dilutions: ELISA: 1/2,000-1/10,000. Western blot: 1/200-1/1,000. Immunoprecipitation: 1/100. Immunohistochemistry: 1/50-1/500.
Background	Lipids (triglyceride (TG), cholesterol (Chol), phospholipids (PL), cholesteryl esters (CE)) are insoluble in plasma. These lipids combine with apolipoproteins to form lipoproteins. This process solubilizes lipids in plasma. Lipoproteins provide a transport system for lipids. They also play an important role in lipoprotein receptor recognition and regulation of certain enzymes in lipoprotein metabolism. Apolipoprotein B plays an essential role in lipid transport and metabolism. Apo B may regulate cholesterol synthesis through its interaction with specific cell membrane receptors and by inhibition of HMG Co A reductase. This enzyme has been identified as the rate controlling enzyme in cholesterol biosynthesis. Apo B may be important in the genesis of atherosclerosis. Apo B is the major protein moiety of all lipoproteins other than high density lipoprotein (HDL) in plasma. Increased levels of apo B100 are associated with an increased risk of coronary artery disease. Apo B exists in human plasma as two isoforms: apo B48 and apo B100. Apo B100 is the major physiological ligand for the LDL receptor. It contains 4536 amino acid residues (Chen et al., 1986; Law et al., 1986). Apo B100 is the full length protein. Its gene has been mapped on the short arm of chromosome 2, with an approximate length of 43 kilobases and 29 exons (Ludwig, et al., 1987). The LDL-binding domain of the molecule is proposed to be located between the aa3129-3532 (Knott, et al., 1986). Apo B100 is synthesised in the liver. It is required for the assembly of very low density lipoproteins (VLDL).
Concentration	1.02 mg/ml (by UV absorbance at 280 nm)
Storage	Store the antibody at 2-8°C prior to opening. Dilute only prior to use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Shelf life: One year from date of opening.
Format	Purification: Immunoaffinity Chromatography. Buffer System: 0.125M Sodium Borate, 0.075M Sodium Chloride, 0.005M EDTA, pH 8.0, with 0.01% (w/v) Sodium Azide as preservative. State: Liquid (sterile filtered) purified Ig fraction. Purified
Specificity	Specificity: This product has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other ApoLipoproteins and human serum proteins to remove any unwanted specificities. Typically less than 1% cross reactivity against other types of apoLipoprotein was detected by ELISA against purified standards. This antibody reacts with Human ApoLipoprotein B and has negligible cross-reactivity with Type A-I, A-II, C-I, C-II, C-III, E and J ApoLipoproteins. Non-specific cross reaction of anti-apoLipoprotein antibodies with other Human serum proteins is negligible. Species: Human.
Gene ID	338
FocusOn and Reviews	FocusOns: Antibodies to Apolipoprotein - FocusOn 036
Targets	Apolipoprotein B (Apo B) antibody

Accessory Products

Proteins and/or Positive Controls

Proteins for Apolipoprotein B (Apo B) (5 products)

Catalog No.	Species	Pres.	Purity		Source
BA054	Apolipoprotein B (Apo B)
	Human	Purified	> 95 %		Plasma		0.5 mg / €220.00
							Acris Antibodies GmbH
BA1030	Apolipoprotein B (Apo B) (APOB 100)
	Human	Purified			Plasma		1 mg / €250.00
							Acris Antibodies GmbH
H00000338-Q01-10	Apolipoprotein B (Apo B)
	Human	Purified					10 µg / €350.00
							Abnova Taiwan Corp.
H00000338-Q01-25	Apolipoprotein B (Apo B)
	Human	Purified					25 µg / €500.00
							Abnova Taiwan Corp.
PY-20102	Apolipoprotein B (Apo B) (ApoB 100)
	Human	Purified	> 80 %		E. coli		1 mg / €550.00
							Genesis Biotech Inc.

Recommended Secondary Antibodies for Apolipoprotein B (Apo B) (6 products)

Catalog No.	Host	Clone/Iso.	Pres.		React.	Applications
R1317AP	Goat IgG (H+L chain) antibody
	Rabbit		AP
							Acris Antibodies GmbH
R1317B	Goat IgG (H+L chain) antibody
	Rabbit		Biotin
							Acris Antibodies GmbH
R1317F	Goat IgG (H+L chain) antibody
	Rabbit		FITC
							Acris Antibodies GmbH
R1317HRP	Goat IgG (H+L chain) antibody
	Rabbit		HRP
							Acris Antibodies GmbH
R1317T	Goat IgG (H+L chain) antibody
	Rabbit		TRITC
							Acris Antibodies GmbH
R1416R	Goat IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Donkey		PE
							Acris Antibodies GmbH

Click here to see all secondary antibodies for Apolipoprotein B (Apo B)

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