Apolipoprotein J (Apo J) - DM2012 | acris-antibodies.com

Product Description for Apolipoprotein J (Apo J)

Mouse anti Human Apolipoprotein J (Apo J) Hs-3.
Presentation: Purified
Product is tested for Frozen Sections, Enzyme Immunoassay, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot.

Properties for Apolipoprotein J (Apo J)

Product Category	Antibodies
Quantity	0.1 mg
Synonyms	40, 40, AAG4, APOJ, Aging-associated gene 4 protein, CLI, Clusterin, Complement cytolysis inhibitor, Complement-associated protein SP-40, FocusOn036, FocusOn130, KUB1, Ku70-binding protein 1, NA1/NA2, SP-40, Testosterone-repressed prostate message 2
Presentation	Purified
Reactivity	Hu
Applications	C, E, ICC/IF, WB
Clonality	Monoclonal
Clone	Hs-3
Host	Mouse
Isotype	IgG1
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	Acris Antibodies GmbH

Datasheet Extract

Immunogen	Swiss Prot Num: P10909 Immunogen: The antibody was prepared against Triton X-100 human sperm extract. Genename: 1191
Antibody type	Ab
Application	ELISA. Immunocytochemistry. Immunofluorescence. Immunohistochemistry. Western Blot.
Background	Clusterin is a 75-80 kD disulfide-linked heterodimeric protein containing about 30% of N-linked carbohydrate rich in sialic acid, but truncated forms targeted to the nucleus have also been identified. The precursor polypeptide chain is cleaved proteolytically to remove the 22-mer secretory signal peptide and subsequently between residues 227/228 to generate the alpha and beta chains. These are assembled antiparallel to give a heterodimeric molecule in which the cysteine-rich centers are linked by five disulfide bridges and are flanked by two predicted coiled-coil alpha-helices and three predicted amphipathic alpha-helices. The six sites of N-linked glycosylation are indicated as yellow spots. Across a broad range of species clusterin shows 70% to 80% of sequence homology. It is ubiquitously expressed in most mammalian tissues and can be found in plasma, milk, urine, cerebrospinal fluid and semen. It is able to bind and form complexes with numerous partners such as immunoglobulins, lipids, heparin, bacteria, complement components, paraoxonase, beta amyloid, leptin and others. Clusterin has been ascribed a plethora of functions such as phagocyte recruitment, aggregation induction, complement attack prevention, apoptosis inhibition, membrane remodelling, lipid transport, hormone transport and/or scavenging, matrix metalloproteinase inhibition. A detailed mechanism of clusterin has not been defined. One tempting hypothesis says that clusterin is an extracellular chaperone protecting cells from stress induced by degraded and misfolded protein precipitates.Clusterin is up- or downregulated on the mRNA or protein level in many pathological and clinically relevant situations including cancer, organ regeneration, infection, Alzheimer disease, retinitis pigmentosa, myocardial infarction, renal tubular damage, autoimmunity and others.
Concentration	1.0 mg/ml
General Readings	1. Zenkel M, Kruse FE, Junemann AG, Naumann GO, Schlotzer-Schrehardt U . Clusterin deficiency in eyes with pseudoexfoliation syndrome may be implicated in the aggregation and deposition of pseudoexfoliative material. Invest Ophthalmol Vis Sci . May;47(5):1982-90 (2006) See: http://www.iovs.org/cgi/content/full/47/5/1982
Storage	Store lyophilized (preferably in a desiccator) at -20°C and in aliquots at -80°C. Reconstituted antibody can be stored at 4°C for a limited period of time; it does not show decline in activity after two weeks at 4°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Format	Purification: Affinity chromatography on protein A followed by DEAE-chromatography Buffer System: 0.05 M phosphate buffer, 0.1 M NaCl, pH 7.2; 15 mM SODIUM AZIDE State: Lyophilized purified IgG Purified Reconstitution: Restore with 0.1 ml of deionized water
Specificity	Specificity: The Anti Human Clusterin, Clone: Hs-3 is a mouse monoclonal antibody against Human Clusterin. Species: Human.
Gene ID	1191
FocusOn and Reviews	FocusOns: Antibodies to Apolipoprotein - FocusOn 036
Targets	Apolipoprotein J (Apo J) antibody

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