Apolipoprotein J (Apo J) - R1037P | acris-antibodies.com

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Product Description for Apolipoprotein J (Apo J)

Goat anti Human Apolipoprotein J (Apo J).
Presentation: Purified
Product is tested for Frozen Sections, Enzyme Immunoassay, Immunoprecipitation, Western blot / Immunoblot, Paraffin Sections.

Properties for Apolipoprotein J (Apo J)

Product Category	Antibodies
Quantity	0.5 mg
Synonyms	40, 40, AAG4, APOJ, Aging-associated gene 4 protein, CLI, Clusterin, Complement cytolysis inhibitor, Complement-associated protein SP-40, FocusOn036, FocusOn130, KUB1, Ku70-binding protein 1, NA1/NA2, SP-40, Testosterone-repressed prostate message 2
Presentation	Purified
Reactivity	Hu
Applications	C, E, IP, P, WB
Clonality	Polyclonal
Host	Goat
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	Acris Antibodies GmbH
Material safety datasheet	MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen	Swiss Prot Num: P10909 Immunogen: ApoLipoprotein Type J was isolated from human plasma by density gradient centrifugation followed by HPLC purification. Genename: 1191
Antibody type	Ab
Application	Has been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for immunoprecipitation and for western blotting for highly sensitive qualitative analysis. Recommended dilutions: ELISA 1:5,000 - 1:10,000. Western blot 1:5,000 - 1:10,000. Immunohistochemistry on frozen and paraffin sections 1:50 - 1:200.
Background	At least 9 distinct polymorphic forms of apolipoproteins are known. The apolipoproteins act as stabilizers of the intact lipoprotein particles. Quantitative measurements of HDL, LDL and VLDL particles in human serum are often used to estimate an individual's relative risk of coronary heart disease. In addition, quantitative immunological measurements of certain apolipoproteins (especially A-1 and B) have been suggested to be more accurate estimators of coronary heart disease than measurements of lipoprotein particles (especially HDL and LDL). Apolipoprotein J is the major secreted product of Sertoli cells and is thought to play a critical role in spermatogenesis. The protein was shown to be a normal constituent of human blood and is also thought to be a control mechanism of the complement cascade. It prevents the binding of a C5b-C7 complex to the membrane of the target cell and in this way inhibits complement-mediated cytolysis. Apolipoprotein J is overexpressed in human prostate and breast cancers and in squamous cell carcinomas. Suppression of Apolipoprotein J renders these cells sensitive to chemotherapeutic drug-mediated apoptosis. It has been proposed that elevated levels of Apolipoprotein J may promote oncogenic transformation and tumor progression by interfering with BAX proapoptotic activities.
Concentration	1.0 mg/ml (by UV absorbance at 280 nm)
Storage	This product is stable at 2-8°C as an undiluted liquid for up to one month. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20°C or below. Avoid repeated freezing and thawing. Dilute only prior to immediate use. Shelf life: one year from despatch.
Format	Purification: Immunoaffinity chromatography Buffer System: 0.125 M Sodium Borate, 0.075M Sodium Chloride, 0.005M EDTA, pH 8.0, containing 0.01% (w/v) Sodium Azide as preservative State: Liquid (sterile filtered) immunoglobulin fraction Purified
Specificity	Specificity: This product has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other apoLipoproteins and human serum proteins to remove any unwanted specificities. Typically less than 1% cross reactivity against other types of apoLipoprotein was detected by ELISA against purified standards. This antibody reacts with apoLipoprotein J and has negligible cross-reactivity with Type A-I, A-II, B, C-I, C-II, C-III and E apoLipoproteins. Non-specific cross reaction of anti-apoLipoprotein antibodies with other human serum proteins is negligible. Species: Human.
Gene ID	1191
FocusOn and Reviews	FocusOns: Antibodies to Apolipoprotein - FocusOn 036 Antibodies for Alzheimer’s Disease - FocusOn 130
Targets	Apolipoprotein J (Apo J) antibody

Accessory Products

Recommended Secondary Antibodies for Apolipoprotein J (Apo J) (6 products)

Catalog No.	Host	Clone/Iso.	Pres.		React.	Applications
R1317AP	Goat IgG (H+L chain) antibody
	Rabbit		AP
							Acris Antibodies GmbH
R1317B	Goat IgG (H+L chain) antibody
	Rabbit		Biotin
							Acris Antibodies GmbH
R1317F	Goat IgG (H+L chain) antibody
	Rabbit		FITC
							Acris Antibodies GmbH
R1317HRP	Goat IgG (H+L chain) antibody
	Rabbit		HRP
							Acris Antibodies GmbH
R1317T	Goat IgG (H+L chain) antibody
	Rabbit		TRITC
							Acris Antibodies GmbH
R1416R	Goat IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Donkey		PE
							Acris Antibodies GmbH

Click here to see all secondary antibodies for Apolipoprotein J (Apo J)

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