Aromatase (376-390) - SM2222P | acris-antibodies.com

Product Description for Aromatase

Mouse anti Chicken, Human, Marmoset, Monkey, Rat Aromatase H4.
Properties: (376-390)
Presentation: Supernatant
Product is tested for Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot, Paraffin Sections.

Properties for Aromatase

Product Category	Antibodies
Quantity	1 ml
Synonyms	ARO1, CYAR, CYP19, CYP19A1, CYPXIX, Cytochrome P450 19A1, Estrogen synthetase, FocusOn003, P450AROM
Presentation	Supernatant
Reactivity	Chk, Hu, Marmoset, Mky, Rt
Applications	ICC/IF, P, WB
Clonality	Monoclonal
Clone	H4
Host	Mouse
Isotype	IgG2a
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	Acris Antibodies GmbH

Datasheet Extract

Immunogen	Swiss Prot Num: P11511 Immunogen: Synthetic peptide corresponding to amino acids 376-390 of Human Aromatase. Genename: 1588
Property	376-390
Antibody type	Ab
Application	Immunocytochemistry (see Ref. 15). Immunohistochemistry on Paraffin Sections: Does not require antigen retrieval using heat treatment. Recommended Positive Control Tissue: Human placenta (see Ref. 11). Western Blot (1/250): Detects a band of approximately 55 kD in human placental extracts (see Ref. 4). P450 aromatase is highly expressed in placental tissue. For tissues where there may be low expression of P450 aromatase, the use of microsomal extracts may improve the staining for Western blots using this antibody (see Ref.1).
Background	Aromatase is a key enzyme in steroidogenesis and plays an important role in sexual differentiation, oestrogen biosynthesis, fertility and carcinogenesis. It is highly conserved amongst mammals, and is highly expressed in placental tissue. Many environmental chemicals may influence aromatase activity and thereby disrupt endocrine function.
Product Citations	Colette S, Lousse JC, Defrère S, Curaba M, Heilier JF, Van Langendonckt A, et al. Absence of aromatase protein and mRNA expression in endometriosis. Hum Reprod. 2009 Sep;24(9):2133-41. doi: 10.1093/humrep/dep199. Epub 2009 Jun 2. PubMed PMID: 19493871. Bender RA, Zhou L, Wilkars W, Fester L, Lanowski JS, Paysen D, et al. Roles of 17ß-estradiol involve regulation of reelin expression and synaptogenesis in the dentate gyrus. Cereb Cortex. 2010 Dec;20(12):2985-95. doi: 10.1093/cercor/bhq047. Epub 2010 Apr 26. PubMed PMID: 20421250. Horling K, Santos AN, Fischer B. The AhR is constitutively activated and affects granulosa cell features in the human cell line KGN. Mol Hum Reprod. 2011 Feb;17(2):104-14. doi: 10.1093/molehr/gaq074. Epub 2010 Sep 7. PubMed PMID: 20823264.
General Readings	Turner KJ, Macpherson S, Millar MR, McNeilly AS, Williams K, Cranfield M, et al. Development and validation of a new monoclonal antibody to mammalian aromatase. J Endocrinol. 2002 Jan;172(1):21-30. PubMed PMID: 11786371. Lu Y, Amleh A, Sun J, Jin X, McCullough SD, Baer R, et al. Ubiquitination and proteasome-mediated degradation of BRCA1 and BARD1 during steroidogenesis in human ovarian granulosa cells. Mol Endocrinol. 2007 Mar;21(3):651-63. Epub 2006 Dec 21. PubMed PMID: 17185394. Zhao D, McBride D, Nandi S, McQueen HA, McGrew MJ, Hocking PM, et al. Somatic sex identity is cell autonomous in the chicken. Nature. 2010 Mar 11;464(7286):237-42. doi: 10.1038/nature08852. PubMed PMID: 20220842. Sirianni R, Chimento A, De Luca A, Zolea F, Carpino A, Rago V, et al. Inhibition of cyclooxygenase-2 down-regulates aromatase activity and decreases proliferation of Leydig tumor cells. J Biol Chem. 2009 Oct 16;284(42):28905-16. doi: 10.1074/jbc.M109.041020. Epub 2009 Aug 13. PubMed PMID: 19679653. Carpino, A. et al. (2007) Detection of aromatase and estrogen receptors (ERalpha, ERbeta1, ERbeta2) in human Leydig cell tumor. Eur. J. Endocrinol. 157: 239-244. Catalano S, Malivindi R, Giordano C, Gu G, Panza S, Bonofiglio D, et al. Farnesoid X receptor, through the binding with steroidogenic factor 1-responsive element, inhibits aromatase expression in tumor Leydig cells. J Biol Chem. 2010 Feb 19;285(8):5581-93. doi: 10.1074/jbc.M109.052670. Epub 2009 Dec 21. PubMed PMID: 20026603. Wu YG, Bennett J, Talla D, Stocco C. Testosterone, not 5α-dihydrotestosterone, stimulates LRH-1 leading to FSH-independent expression of Cyp19 and P450scc in granulosa cells. Mol Endocrinol. 2011 Apr;25(4):656-68. doi: 10.1210/me.2010-0367. Epub 2011 Jan 27. PubMed PMID: 21273442. Lu Y, Kang T, Hu Y. BRCA1/BARD1 complex interacts with steroidogenic factor 1--A potential mechanism for regulation of aromatase expression by BRCA1. J Steroid Biochem Mol Biol. 2011 Jan;123(1-2):71-8. doi: 10.1016/j.jsbmb.2010.11.006. Epub 2010 Nov 16. PubMed PMID: 21087664. Barone I, Cui Y, Herynk MH, Corona-Rodriguez A, Giordano C, Selever J, et al. Expression of the K303R estrogen receptor-alpha breast cancer mutation induces resistance to an aromatase inhibitor via addiction to the PI3K/Akt kinase pathway. Cancer Res. 2009 Jun 1;69(11):4724-32. doi: 10.1158/0008-5472.CAN-08-4194. PubMed PMID: 19487288. Ghosh S, Lu Y, Hu Y. A Role of CREB in BRCA1 Constitutive Promoter Activity and Aromatase Basal Expression. Int J Biomed Sci. 2008 Dec 15;4(4):260-265. PubMed PMID: 19568323. Jeong JH, Jung YK, Kim HJ, Jin JS, Kim HN, Kang SM, et al. The gene for aromatase, a rate-limiting enzyme for local estrogen biosynthesis, is a downstream target gene of Runx2 in skeletal tissues. Mol Cell Biol. 2010 May;30(10):2365-75. doi: 10.1128/MCB.00672-09. Epub 2010 Mar 15. PubMed PMID: 20231365. Rago, V. et al. (2005) Cytochrome P450 aromatase expression in human seminoma. Reprod Biol Endocrinol. 3: 72. Schmidt M, Weidler C, Naumann H, Anders S, Schölmerich J, Straub RH. Androgen conversion in osteoarthritis and rheumatoid arthritis synoviocytes--androstenedione and testosterone inhibit estrogen formation and favor production of more potent 5alpha-reduced androgens. Arthritis Res Ther. 2005;7(5):R938-48. Epub 2005 Jun 10. PubMed PMID: 16207335. Stabile, L.P. et al. (2012) Prevention of Tobacco Carcinogen-Induced Lung Cancer in Female Mice Using Anti-Estrogens. Carcinogenesis. Aug 2. [Epub ahead of print] Oki Y, Ono H, Motohashi T, Sugiura N, Nobusue H, Kano K. Dedifferentiated follicular granulosa cells derived from pig ovary can transdifferentiate into osteoblasts. Biochem J. 2012 Oct 15;447(2):239-48. PubMed PMID: 22839299.
Storage	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Format	Preservatives: 0.09% Sodium Azide State: Liquid 10 x Concentrated Tissue Culture Supernatant Supernatant
Specificity	Specificity: SM2222P recognises a conserved epitope within Cytochrome P450 Aromatase (P450 arom). Species: Human, Rat, Chicken and Monkey (Marmoset).
Gene ID	1588
FocusOn and Reviews	FocusOns: Antibodies to P450 Aromatase - FocusOn 003
Targets	Aromatase antibody

Accessory Products

Proteins and/or Positive Controls

Proteins for Aromatase (6 products)

Catalog No.	Species	Pres.	Source
H00001588-P02	Aromatase
	Human	Purified		10 µg / €420.00
				Abnova Taiwan Corp.
H00001588-P02-10	Aromatase
	Human		in vitro transl.	10 µg / €350.00
				Abnova Taiwan Corp.
H00001588-P02-25	Aromatase
	Human		in vitro transl.	25 µg / €500.00
				Abnova Taiwan Corp.
H00001588-P03	Aromatase
	Human	Purified		10 µg / €330.00
				Abnova Taiwan Corp.
H00001588-P03-10	Aromatase
	Human	Purified		10 µg / €350.00
				Abnova Taiwan Corp.
H00001588-P03-25	Aromatase
	Human	Purified		25 µg / €500.00
				Abnova Taiwan Corp.

Positive controls for Aromatase (1 products)

Catalog No.	Species
NBL1-09674	Aromatase Lysate
		0.1 mg / €370.00
		Novus Biologicals Inc.

Recommended Secondary Antibodies for Aromatase (8 products)

Catalog No.	Host	Pres.
R1253AP	Mouse IgG (H+L chain) antibody
	Rabbit	AP
			Acris Antibodies GmbH
R1253B	Mouse IgG (H+L chain) antibody
	Rabbit	Biotin
			Acris Antibodies GmbH
R1253F	Mouse IgG (H+L chain) antibody
	Rabbit	FITC
			Acris Antibodies GmbH
R1253HRP	Mouse IgG (H+L chain) antibody
	Rabbit	HRP
			Acris Antibodies GmbH
R1253TR	Mouse IgG (H+L chain) antibody
	Rabbit	Texas Red
			Acris Antibodies GmbH
R1403R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Rabbit	PE
			Acris Antibodies GmbH
R1405R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Goat	PE
			Acris Antibodies GmbH
R1455R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Donkey	PE
			Acris Antibodies GmbH