ATP2A1 / SERCA1

Product Description for ATP2A1 / SERCA1

Mouse anti Canine, Human, Mouse, Rabbit ATP2A1 / SERCA1 VE121G9.
Presentation: Ascites
Product is tested for Immunocytochemistry/Immunofluorescence, Paraffin Sections, Western blot / Immunoblot.

Properties for ATP2A1 / SERCA1

Product Category	Antibodies
Quantity	0.1 ml
Synonyms	Calcium pump 1 ATPase, Calcium-transporting ATPase sarcoplasmic reticulum type, Endoplasmic reticulum class 1/2 Ca(2+) ATPase, Review 012, SR Ca(2+)-ATPase 1, Sarcoplasmic/endoplasmic reticulum calcium ATPase 1, fast twitch skeletal muscle isoform
Presentation	Ascites
Reactivity	Can, Hu, Ms, Rb
Applications	ICC/IF, P, WB
Clonality	Monoclonal
Clone	VE121G9
Host	Mouse
Isotype	IgG1
Shipping to	Not USA/Canada
PDF datasheet	View Datasheet
Manufacturer	Novus Biologicals Inc.

Datasheet Extract

Immunogen	Swiss Prot Num: O14983 Immunogen: Purified rabbit skeletal muscle sarcoplasmic reticulum. Genename: 487
Antibody type	Ab
Application	immunofluorescence, Western Blot, Optimal dilutions / concentrations should be determined by the end user. By Western blot, this antibody detects an ~110 kDa protein representing SERCA 1 ATPase in mouse muscle extracts. Immunofluorescence staining of SERCA 1 ATPase in canine skeletal muscle results in strong labeling of the entire type II (fast) myofiber.This antibody has also been shown to inhibit the crystallization of SERCA ATPase induced by vanadate., immunofluorescence 1/500, Western Blot 1/2500
Background	ATP dependent calcium pumps are responsible, in part, for the maintenance of low cytoplasmic free calcium concentrations. The ATP pumps that reside in intracellular organelles are encoded by a family of structurally related enzymes, termed the sarcoplasmic or endoplasmic reticulum calcium (SERCA) ATPases. The SERCA 1 gene is exclusively expressed in type II (fast) skeletal muscle. The SERCA 2 gene is subject to tissue dependent processing which is responsible for the generation of SERCA 2a muscle-specific form expressed in type I (slow) skeletal, cardiac and smooth muscle and the SERCA 2b isoform expressed in all cell types. The SERCA 3 gene is not as well characterized and is found in non-muscle cells.
General Readings	General / background references:Molnar E et al. Immunological relatedness of the sarcoplasmic reticulum Ca(2+)-ATPase and the Na+,K(+)-ATPase. Biochim Biophys Acta 1103:281-95 (1992). Molnar E et al. The binding of monoclonal and polyclonal antibodies to the Ca2(+)-ATPase of sarcoplasmic reticulum: effects on interactions between ATPase molecules. Biochim Biophys Acta 1023:147-67 (1990). Knudson CM et al. Specific absence of the alpha 1 subunit of the dihydropyridine receptor in mice with muscular dysgenesis. J Biol Chem 264:1345-8 (1989).
Storage	Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Format	Purification: Ascites Buffer System: 0.05% Sodium Azide Purity: Ascites State: Ascites
Specificity	Specificity: This antibody recognizes an epitope between amino acid residues 506 and the C-terminus of rabbit skeletal muscle ATPase, a region that is exposed in native sarcoplasmic reticulum., Detects Sarcoplasmic or Endoplasmic Reticulum Calcium 1 (SERCA 1) ATPase. Species: Canine, Human, Mouse, Rabbit
Gene ID	487
FocusOn and Reviews	Reviews: Antibodies to ATPase Enzymes - Product Review 12
Targets	ATP2A1 / SERCA1 antibody

Accessory Products

Proteins and/or Positive Controls

Proteins for ATP2A1 / SERCA1 (3 products)

Catalog No.	Species	Pres.	Source
H00000487-P01	ATP2A1 / SERCA1
	Human	Purified		2 µg / €250.00
				Abnova Taiwan Corp.
H00000487-Q01-10	ATP2A1 / SERCA1
	Human		in vitro transl.	10 µg / €350.00
				Abnova Taiwan Corp.
H00000487-Q01-25	ATP2A1 / SERCA1
	Human		in vitro transl.	25 µg / €500.00
				Abnova Taiwan Corp.

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