ATP4B

Product Description for ATP4B

Mouse anti Bovine, Canine, Ferret, Human, Mouse, Porcine, Rabbit, Rat ATP4B 2G11.
Presentation: Ascites
Product is tested for Frozen Sections, ChIP assay, Immunocytochemistry/Immunofluorescence, Paraffin Sections, Immunoprecipitation, Western blot / Immunoblot.

Properties for ATP4B

Product Category	Antibodies
Quantity	0.1 ml
Synonyms	Gastric H(+)/K(+) ATPase subunit beta, Potassium-transporting ATPase subunit beta, Proton pump ATPase beta chain, Review 012
Presentation	Ascites
Reactivity	Bov, Can, Ft, Hu, Ms, Por, Rb, Rt
Applications	C, ChIP, ICC/IF, IP, P, WB
Clonality	Monoclonal
Clone	2G11
Host	Mouse
Isotype	IgG1
Shipping to	Not USA/Canada
PDF datasheet	View Datasheet
Manufacturer	Novus Biologicals Inc.

Datasheet Extract

Immunogen	Swiss Prot Num: P51164 Immunogen: Purified 34 kDa core peptide from deglycosylated hog gastric microsomes. Genename: 496
Antibody type	Ab
Application	immunohistochemistry, Western Blot, Immunohistochemistry: use at a dilution of 1/2000 Western Blot: use at a dilution of 1/4000Not tested in other applications.Optimal dilutions / concentrations should be determined by the end user.By Western blot, this antibody detects various forms of the beta-subunit, including a 60 to 80 kDa glycosylated protein, a 52 kDa beta-subunit precursor, and the 34 kDa core peptide from rabbit stomach extracts.Immunohistochemical staining of hydrogen/potassium ATPase in rabbit gastric glands with this antibody results in specific staining of parietal cells.This antibody has been shown to inhibit the enzymatic activity of the hydrogen/potassium ATPase and to alter the affinity of the cytoplasmic potassium binding site. , immunohistochemistry Fr, Western Blot
Background	The hydrogen/potassium ATPase, or gastric proton pump, belongs to a family of P-type cation-transporting ATPases. This family of ATPases shares a number of functional and structural similarities including the common feature of consisting of an alpha and beta subunit. Like the ubiquitous sodium/potassium ATPase, the hydrogen/potassium ATPase consists of a large transmembrane catalytic subunit, termed the alpha-subunit which contains sites for ATP binding and phosphorylation, and an associated smaller glycoprotein, termed the beta-subunit which may play a role in maintaining the structural and functional integrity of the complex.
General Readings	This product has been used in:Chow DC & Forte JG Characterization of the beta-subunit of the H(+)-K(+)-ATPase using an inhibitory monoclonal antibody. Am J Physiol 265:C1562-70 (1993). General / background references:Cougnon M et al. The rat distal colon P-ATPase alpha subunit encodes a ouabain-sensitive H+, K+-ATPase. J Biol Chem 271:7277-80 (1996).
Storage	Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Format	Purification: IgG purified Buffer System: 0.05% Sodium Azide Purity: IgG purified State: Ascites
Specificity	Specificity: This antibody recognizes an epitope between amino acid residues 1-13 or 15-28 located on the cytoplasmic side of the beta-subunit., Detects the beta-subunit of hydrogen/potassium ATPase. Species: Canine, Ferret, Mouse, Rabbit, Rat
Gene ID	496
FocusOn and Reviews	Reviews: Antibodies to ATPase Enzymes - Product Review 12
Targets	ATP4B antibody

Accessory Products

Proteins and/or Positive Controls

Proteins for ATP4B (3 products)

Catalog No.	Species	Pres.	Source
H00000496-P01-02	ATP4B
	Human		in vitro transl.	2 µg / €250.00
				Abnova Taiwan Corp.
H00000496-Q01-10	ATP4B
	Human	Purified		10 µg / €350.00
				Abnova Taiwan Corp.
H00000496-Q01-25	ATP4B
	Human	Purified		25 µg / €500.00
				Abnova Taiwan Corp.

Positive controls for ATP4B (3 products)

Catalog No.	Species
H00000496-T02	ATP4B 293T Cell Transient Overexpression Lysate(Denatured)
		0.1 ml / €240.00
		Abnova Taiwan Corp.
H00000496-T01	ATP4B Lysate(Denatured)
		0.1 ml / €240.00
		Abnova Taiwan Corp.
NBL1-07815	Hydrogen Potassium ATPase Beta Lysate
		0.1 mg / €370.00
		Novus Biologicals Inc.

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R1403R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
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R1405R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
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R1455R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
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