ATP4B

Product Description for ATP4B

Mouse anti Bovine, Canine, Ferret, Human, Mouse, Porcine, Rabbit, Rat ATP4B 2G11.
Presentation: Ascites
Product is tested for Frozen Sections, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western blot / Immunoblot, Paraffin Sections.

Properties for ATP4B

Product Category	Antibodies
Quantity	0.1 ml
Synonyms	Gastric H(+)/K(+) ATPase subunit beta, Potassium-transporting ATPase subunit beta, Proton pump ATPase beta chain, Review 012
Presentation	Ascites
Reactivity	Bov, Can, Ft, Hu, Ms, Por, Rb, Rt
Applications	C, ICC/IF, IP, P, WB
Clonality	Monoclonal
Clone	2G11
Host	Mouse
Isotype	IgG1
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	Acris Antibodies GmbH

Datasheet Extract

Immunogen	Swiss Prot Num: P51164 Immunogen: Purified 34 kDa core peptide from deglycosylated hog gastric microsomes. Genename: 496
Antibody type	Ab
Application	Western Blot: 1/4,000. Immunoprecipitation: Assay dependent. Immunohistochemistry on Paraffin/Frozen Sections: 1/2,000. Immunofluorescence: 1/2,000.
Background	The hydrogen/potassium ATPase, or gastric proton pump, belongs to a family of P-type cation-transporting ATPases. This family of ATPases shares a number of functional and structural similarities including the common feature of consisting of an alpha and beta subunit. Like the ubiquitous sodium/potassium ATPase, the hydrogen/potassium ATPase consists of a large transmembrane catalytic subunit, termed the alpha- subunit which contains sites for ATP binding and phosphorylation, and an associated smaller glycoprotein, termed the beta-subunit which may play a role in maintaining the structural and functional integrity of the complex.
Product Citations	Dürr KL, Tavraz NN, Dempski RE, Bamberg E, Friedrich T. Functional significance of E2 state stabilization by specific alpha/beta-subunit interactions of Na,K- and H,K-ATPase. J Biol Chem. 2009 Feb 6;284(6):3842-54. doi: 10.1074/jbc.M808101200. Epub 2008 Dec 8. PubMed PMID: 19064992.
General Readings	JBC, 277 (51): 50030-50035, 2002. Am J. Physiol Cell Physiol 281: C407-C417, 2001. JBC, 277(11): 9468-9473, 2002 Am J. Physiol Cell Physiol, 277(46): C361-C372, 1999 Biochem., 40: 5765-5776, 2001 CANCER RESEARCH 64, 3687 - 3693, May 15, 2004 THE JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS Vol. 309, No. 2, May 2004 Mol. Pathol., Vol 56: 150-155, Jun 2003 Am J Physiol Cell Physiol, Vol 285: C662 - C673, Sept 2003 JBC 277(51):50030-50035, 2002. J. Biol. Chem., Aug 2006; 281: 23740 - 23747. Am J Physiol Cell Physiol, Sep 2000; 279: C833 - C851. Am J Physiol Cell Physiol, Sep 1999; 277: C361 - C372. Am J Physiol Renal Physiol, Apr 1998; 274: 665. Genes Cells, Jun 2006; 11: 623 - 631. AJP-GI Vol 285, 1242-1248, Dec 2003 JCB Vol. 169, No. 1 21-28 Apr 2005 JHC DOI:10.1369/jhc.5A6806.2005 J. Histochem. Cytochem. Vol. 52(1), Jan 2004 Development May 2005 doi:10.1242/dev.01808 J. Pharm Exp Therapeutics Vol. 310, No. 2, 477-487 Aug 2004 Cancer Research Vol 64, 8160-8166, Nov 2004 Am. J. Pathol., Vol 162: 1905-1912, Jun 2003 Am. J. Physiol., 265(34): C1562-1570, 1993. J. Biol. Chem., 271(13): 7277-7280, 1996. Am J Physiol Cell Physiol 286, C1229-C1237, Jun 2004 Diabetes Care Vol 27, 1387-1393, Jun 2004
Storage	Store the antibody (undiluted) at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Format	Buffer System: PBS containing 0.05% Sodium Azide as preservative. State: Liquid Ascites Ascites
Specificity	Specificity: This antibody detects the beta-subunit of hydrogen/potassium ATPase from bovine, human, canine, porcine, rabbit, mouse, ferret, and rat tissues. This antibody has been successfully used in Western blot, Immunohistochemistry and immunoprecipitation procedures (ChIP). By Western blot, this antibody detects various forms of the beta-subunit, including a 60 to 80 kDa glycosylated protein, a 52 kDa beta-subunit precursor, and the 34 kDa core peptide from rabbit stomach extracts. Immunohistochemical staining of hydrogen/potassium ATPase in rabbit gastric glands with SM5117 results in specific staining of parietal cells. SM5117 has been shown to inhibit the enzymatic activity of the hydrogen/potassium ATPase and to alter the affinity of the cytoplasmic potassium binding site. The SM5117 antigen is purified 34 kDa core peptide from deglycosylated hog gastric microsomes. This antibody recognizes an epitope between amino acid residues 1-13 or 15-28 located on the cytoplasmic side of the beta-subunit. Species: Human, Porcine, Canine, Ferret, Rat, Mouse, Rabbit and Bovine.
Gene ID	496
FocusOn and Reviews	Reviews: Antibodies to ATPase Enzymes - Product Review 12
Targets	ATP4B antibody

Accessory Products

Proteins and/or Positive Controls

Proteins for ATP4B (3 products)

Catalog No.	Species	Pres.	Source
H00000496-P01-02	ATP4B
	Human		in vitro transl.	2 µg / €250.00
				Abnova Taiwan Corp.
H00000496-Q01-10	ATP4B
	Human	Purified		10 µg / €350.00
				Abnova Taiwan Corp.
H00000496-Q01-25	ATP4B
	Human	Purified		25 µg / €500.00
				Abnova Taiwan Corp.

Positive controls for ATP4B (3 products)

Catalog No.	Species
H00000496-T02	ATP4B 293T Cell Transient Overexpression Lysate(Denatured)
		0.1 ml / €240.00
		Abnova Taiwan Corp.
H00000496-T01	ATP4B Lysate(Denatured)
		0.1 ml / €240.00
		Abnova Taiwan Corp.
NBL1-07815	Hydrogen Potassium ATPase Beta Lysate
		0.1 mg / €370.00
		Novus Biologicals Inc.

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