Bcl-2

Product Description for Bcl-2

Rabbit anti Human Bcl-2.
Presentation: Aff - Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot, Paraffin Sections.

Properties for Bcl-2

Product Category	Antibodies
Quantity	50 µg
Synonyms	Apoptosis regulator Bcl-2, B-Cell CLL/lymphoma 2, BCL2, Bcl-2 alpha, Review 004
Presentation	Aff - Purified
Reactivity	Hu
Applications	ICC/IF, P, WB
Clonality	Polyclonal
Host	Rabbit
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	Acris Antibodies GmbH

Datasheet Extract

Immunogen	Swiss Prot Num: P10415 Immunogen: Peptide sequence around amino acids 54~58 (G-H-T-P-H)derived from Human BCL-2 Genename: 596
Antibody type	Ab
Add. information	Molecular Weight: 26 kDa
Application	Western Blot: 1/500-1/1000. Incubate membrane with diluted antibody in 5% nonfat milk, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunofluorescence: 1/100-1/200. Immunohistochemistry on Paraffin Sections: 1/50~1/100.
Background	BCL2 is an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants (alpha and beta) produced by alternate splicing, differ in their C-terminal ends. BCL2 suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. It regulates cell death by controlling the mitochondrial membrane permeability. It appears to function in a feedback loop system with caspases. BCL2 inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF1). It can form homodimers, and heterodimers with BAX, BAD, BAK and BclX(L). Heterodimerization with BAX requires intact BH1 and BH2 domains, and is necessary for anti-apoptotic activity. Also interacts with APAF1, RAF1, TP53BP2, BBC3, BCL2L1 and BNIPL.
Concentration	1.0 mg/ml
General Readings	Ling, Y. H. et al. (1998) J. Biol. Chem. 273, 18984-18991. Huang, S.J. and Cidlowski, J.A. (2002) FASEB 16, 825-832. Deng, X. et al. (2001) J. Biol. Chem. 276, 23681-23688. Huang ST,et al. (2002) FASEB J Jun; 16(8): 825-32. Yamamoto, K. et al. (1999) Mol. Cell. Biol. 19, 8469-8478.
Storage	Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing. Shelf life: One year from despatch.
Format	Purification: Immunoaffinity Chromatography using epitope-specific peptide. Buffer System: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol. State: Liquid purified Ig fraction. Aff - Purified
Specificity	Specificity: This antibody detects endogenous levels of total BCL-2 protein. Species: Human.
Gene ID	596
FocusOn and Reviews	Reviews: Antibodies to bcl-2 Protein Family - Product Review 04
Targets	Bcl-2 antibody

Accessory Products

Proteins and/or Positive Controls

Proteins for Bcl-2 (9 products)

Catalog No.	Species	Pres.	Purity	Source
AR09177PU-L	Bcl-2 (1-211, His-tag)
	Human	Purified	> 90 %	E. coli	0.5 mg / €1,000.00
					Acris Antibodies GmbH
AR09177PU-N	Bcl-2 (1-211, His-tag)
	Human	Purified	> 90 %	E. coli	0.1 mg / €370.00
					Acris Antibodies GmbH
GTX27983	Bcl-2 Control Peptide
					0.1 mg / €230.00
					GeneTex Inc.
H00000596-P01-10	Bcl-2
	Human	Purified			10 µg / €350.00
					Abnova Taiwan Corp.
H00000596-P01-25	Bcl-2
	Human	Purified			25 µg / €500.00
					Abnova Taiwan Corp.
H00000596-Q01-10	Bcl-2
	Human			in vitro transl.	10 µg / €350.00
					Abnova Taiwan Corp.
H00000596-Q01-25	Bcl-2
	Human			in vitro transl.	25 µg / €500.00
					Abnova Taiwan Corp.
PR27054-1	Bcl-2 (1-218)
	Human	Purified	> 95 %	E. coli	1 µg / €150.00
					Neuromics Antibodies
PR27054-5	Bcl-2 (1-218)
	Human	Purified	> 95 %	E. coli	5 µg / €280.00
					Neuromics Antibodies

Recommended Secondary Antibodies for Bcl-2 (6 products)

Catalog No.	Host	Pres.
R1364AP	Rabbit IgG (H+L chain) antibody
	Goat	AP
			Acris Antibodies GmbH
R1364B	Rabbit IgG (H+L chain) antibody
	Goat	Biotin
			Acris Antibodies GmbH
R1364F	Rabbit IgG (H+L chain) antibody
	Goat	FITC
			Acris Antibodies GmbH
R1364HRP	Rabbit IgG (H+L chain) antibody
	Goat	HRP
			Acris Antibodies GmbH
R1364T	Rabbit IgG (H+L chain) antibody
	Goat	TRITC
			Acris Antibodies GmbH
R1427R	Rabbit IgG F(ab)2 Fragment (H+L chain) antibody
	Donkey	PE
			Acris Antibodies GmbH