Bcl-2-like 11 - AM12008PU-N | acris-antibodies.com

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Product Description for Bcl-2-like 11

Hamster anti Mouse Bcl-2-like 11 Ham151-149.
Presentation: Aff - Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western blot / Immunoblot, Flow Cytometry.

Properties for Bcl-2-like 11

Product Category	Antibodies
Quantity	0.1 mg
Synonyms	BCL2L11, BIM, Bcl2-L-11, Bcl2-interacting mediator of cell death, BimEL, BimL, BimS, Review 004
Presentation	Aff - Purified
Reactivity	Ms
Applications	F, ICC/IF, IP, WB
Clonality	Monoclonal
Clone	Ham151-149
Host	Hamster
Isotype	IgG1
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	Acris Antibodies GmbH

Datasheet Extract

Immunogen	Swiss Prot Num: O54918 Immunogen: Mouse BimL (w/o transmembrane region) + Bcl-xL (w/o transmembrane region)-6-his tag, purified from Hi5 cells Genename: 12125
Antibody type	Ab
Application	Flow cytometry: 10 µg/ml (Cells permeabilized with 0.03% saponin). Immunoprecipitation: 1-2 µg/100-500 µg of protein in 1 ml lysate. Western blot: 1-2 µg/ml (reducing conditions). Immunocytochemistry on permeabilized cells.
Background	Bim (Bcl2-interacting mediator) is a pro-apoptotic protein of BH3 domain-only subgroup of the Bcl2 family. It has important roles in initiation of apoptosis in response to many death stimuli. Bim is an important regulator of B and T cell negative selection and is also an essential regulator of T cell apoptosis during termination of an immune response. Bim is constitutively expressed in many cell types but it is maintained in an inactive form through binding to the microtubule-associated dynein motor complex.
Concentration	1.0 mg/ml
General Readings	Hughes P, Bouillet P, Strasser A. Role of Bim and other Bcl-2 family members in autoimmune and degenerative diseases. Curr Dir Autoimmun. 2006;9:74-94. PubMed PMID: 16394656. Snow AL, Oliveira JB, Zheng L, Dale JK, Fleisher TA, Lenardo MJ. Critical role for BIM in T cell receptor restimulation-induced death. Biol Direct. 2008 Aug 20;3:34. doi: 10.1186/1745-6150-3-34. PubMed PMID: 18715501. Ludwinski MW, Sun J, Hilliard B, Gong S, Xue F, Carmody RJ, et al. Critical roles of Bim in T cell activation and T cell-mediated autoimmune inflammation in mice. J Clin Invest. 2009 Jun;119(6):1706-13. doi: 10.1172/JCI37619. PubMed PMID: 19411758. Yang Y, Zhao Y, Liao W, Yang J, Wu L, Zheng Z, et al. Acetylation of FoxO1 activates Bim expression to induce apoptosis in response to histone deacetylase inhibitor depsipeptide treatment. Neoplasia. 2009 Apr;11(4):313-24. PubMed PMID: 19308286. Wang X, Xing D, Liu L, Chen WR. BimL directly neutralizes Bcl-xL to promote Bax activation during UV-induced apoptosis. FEBS Lett. 2009 Jun 18;583(12):1873-9. doi: 10.1016/j.febslet.2009.04.045. Epub 2009 May 8. PubMed PMID: 19427863. Oliver PM, Wang M, Zhu Y, White J, Kappler J, Marrack P: Loss of Bim allows precursor B cell survival but not precursor B cell differentiation in the absence of interleukin 7. J Exp Med. 2004 Nov 1;200(9):1179-87. Jorgensen TN, McKee A, Wang M, Kushnir E, White J, Refaeli Y, et al. Bim and Bcl-2 mutually affect the expression of the other in T cells. J Immunol. 2007 Sep 15;179(6):3417-24. PubMed PMID: 17785775.
Storage	Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Format	Purification: Protein A affinity chromatography (> 95% pure by SDS-PAGE) Buffer System: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 State: Liquid Ig fraction Aff - Purified
Species Reactivity	Species reactivity (tested): Mouse
Specificity	Specificity: This antibody reacts with Bim, a 19-24 kDa pro-apoptotic protein (Bcl-2 family) which regulates immunological responses.
Gene ID	12125
FocusOn and Reviews	Reviews: Antibodies to bcl-2 Protein Family - Product Review 04
Targets	Bcl-2-like 11 antibody

Accessory Products

Recommended Secondary Antibodies for Bcl-2-like 11 (5 products)

Catalog No.	Host	Clone/Iso.	Pres.		React.	Applications
R1327AP	Hamster IgG (H+L chain) antibody
	Rabbit		AP
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R1327B	Hamster IgG (H+L chain) antibody
	Rabbit		Biotin
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R1327F	Hamster IgG (H+L chain) antibody
	Rabbit		FITC
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R1327HRP	Hamster IgG (H+L chain) antibody
	Rabbit		HRP
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R1327T	Hamster IgG (H+L chain) antibody
	Rabbit		TRITC
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Click here to see all secondary antibodies for Bcl-2-like 11

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