Beclin-1 (N-term) - AP20054PU-N | acris-antibodies.com

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Product Description for Beclin-1

Rabbit anti Human, Mouse, Rat Beclin-1.
Properties: (N-term)
Presentation: Aff - Purified
Product is tested for Enzyme Immunoassay, Paraffin Sections, Western blot / Immunoblot.

Properties for Beclin-1

Product Category	Antibodies
Quantity	0.1 mg
Synonyms	BECN1, Coiled-coil myosin-like BCL2-interacting protein, GT197, Protein GT197
Presentation	Aff - Purified
Reactivity	Hu, Ms, Rt
Applications	E, P, WB
Clonality	Polyclonal
Host	Rabbit
Isotype	IgG
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	Acris Antibodies GmbH

Datasheet Extract

Immunogen	Swiss Prot Num: Q14457 Immunogen: Beclin-1 antibody was raised against a 17 amino acid peptide from near the amino terminus of Human Beclin-1. Genename: 8678
Property	N-term
Antibody type	Ab
Control peptide	AP20054CP-N
Application	ELISA. Western blot: Beclin-1 antibody can be used for the detection of Beclin-1 at 0.5–1 μg/ml. Positive Control: 293 Cell Lysate. Immunohistochemistry on Paraffin Sections. Positive Control: Rat Brain Tissue.
Background	Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells (1,2). Beclin-1, a coiled-coil Bcl-2-interacting protein homologous to the yeast autophagy gene apg6 (3,4), is a mammalian autophagy gene that can inhibit tumorigenesis and is expressed at reduced levels in human breast carcinoma, suggesting that defects in autophagy proteins may contribute to the development or progression of tumors (5). Bcl-2 can bind to Beclin-1 and inhibit Beclin-1-dependent autophagy in yeast and mammalian cells, suggesting that Bcl-2 functions as an anti-autophagy protein as well as an anti-apoptotic protein, which helps maintain autophagy at levels that are more compatible with cell survival rather than cell death (6).
General Readings	Gozuacik D, Kimchi A. Autophagy as a cell death and tumor suppressor mechanism. Oncogene. 2004 Apr 12;23(16):2891-906. PubMed PMID: 15077152. Kisen GO, Tessitore L, Costelli P, Gordon PB, Schwarze PE, Baccino FM, et al. Reduced autophagic activity in primary rat hepatocellular carcinoma and ascites hepatoma cells. Carcinogenesis. 1993 Dec;14(12):2501-5. PubMed PMID: 8269618. Liang XH, Kleeman LK, Jiang HH, Gordon G, Goldman JE, Berry G, et al. Protection against fatal Sindbis virus encephalitis by beclin, a novel Bcl-2-interacting protein. J Virol. 1998 Nov;72(11):8586-96. PubMed PMID: 9765397. Kametaka S, Okano T, Ohsumi M, Ohsumi Y. Apg14p and Apg6/Vps30p form a protein complex essential for autophagy in the yeast, Saccharomyces cerevisiae. J Biol Chem. 1998 Aug 28;273(35):22284-91. PubMed PMID: 9712845. Liang XH, Jackson S, Seaman M, Brown K, Kempkes B, Hibshoosh H, et al. Induction of autophagy and inhibition of tumorigenesis by beclin 1. Nature. 1999 Dec 9;402(6762):672-6. PubMed PMID: 10604474. Pattingre S, Tassa A, Qu X, Garuti R, Liang XH, Mizushima N, et al. Bcl-2 antiapoptotic proteins inhibit Beclin 1-dependent autophagy. Cell. 2005 Sep 23;122(6):927-39. PubMed PMID: 16179260.
Storage	Store undiluted at 2-8°C. Antibodies should not be exposed to prolonged high temperatures. Shelf life: one year from despatch.
Format	Purification: Immunoaffinity Chromatography Buffer System: PBS containing 0.02% Sodium Azide as preservative State: Liquid purified IgG fraction Aff - Purified
Specificity	Specificity: Recognizes Beclin-1 (N-term). Species: Human, Mouse and Rat.
Gene ID	8678
FocusOn and Reviews	FocusOns: Autophagy - Recycling as a Survival Factor - FocusOn128
Targets	Beclin-1 antibody

Accessory Products

Proteins and/or Positive Controls

Proteins for Beclin-1 (4 products)

Catalog No.	Species	Pres.	Purity		Source
H00008678-P01-10	Beclin-1
	Human	Purified					10 µg / €350.00
							Abnova Taiwan Corp.
H00008678-P01-25	Beclin-1
	Human	Purified					25 µg / €500.00
							Abnova Taiwan Corp.
H00008678-Q01-10	Beclin-1
	Human				in vitro transl.		10 µg / €350.00
							Abnova Taiwan Corp.
H00008678-Q01-25	Beclin-1
	Human				in vitro transl.		25 µg / €500.00
							Abnova Taiwan Corp.

Positive controls for Beclin-1 (1 products)

Catalog No.	Species	Pres.	Purity		Source
NBL1-07966	Beclin 1 Lysate
							0.1 mg / €370.00
							Novus Biologicals Inc.

Recommended Secondary Antibodies for Beclin-1 (6 products)

Catalog No.	Host	Clone/Iso.	Pres.		React.	Applications
R1364AP	Rabbit IgG (H+L chain) antibody
	Goat		AP
							Acris Antibodies GmbH
R1364B	Rabbit IgG (H+L chain) antibody
	Goat		Biotin
							Acris Antibodies GmbH
R1364F	Rabbit IgG (H+L chain) antibody
	Goat		FITC
							Acris Antibodies GmbH
R1364HRP	Rabbit IgG (H+L chain) antibody
	Goat		HRP
							Acris Antibodies GmbH
R1364T	Rabbit IgG (H+L chain) antibody
	Goat		TRITC
							Acris Antibodies GmbH
R1427R	Rabbit IgG F(ab)2 Fragment (H+L chain) antibody
	Donkey		PE
							Acris Antibodies GmbH

Click here to see all secondary antibodies for Beclin-1

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