c-Myc Epitope Tag (EQKLISEEDL) - SM1863B

Product Description for c-Myc Epitope Tag (EQKLISEEDL)

Mouse anti c-Myc Epitope Tag (EQKLISEEDL) 9E10.
Presentation: Biotin
Product is tested for Frozen Sections, Enzyme Immunoassay, Western blot / Immunoblot, Flow Cytometry, Paraffin Sections.

Properties for c-Myc Epitope Tag (EQKLISEEDL)

Product Category	Antibodies
Quantity	0.1 mg
Synonyms	c-myc tag, myc tag, myc-tag
Presentation	Biotin
Applications	C, E, F, P, WB
Clonality	Monoclonal
Clone	9E10
Host	Mouse
Isotype	IgG1
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	Acris Antibodies GmbH

Datasheet Extract

Immunogen	Swiss Prot Num: P01106 Immunogen: Synthetic peptide sequence AEEQKLISEEDLL corresponding to the C-terminal region of Human c-myc. Spleen cells from immunised Balb/c mice were fused with cells of the myeloma cell line. Genename: 4609
Antibody type	Ab
Application	ELISA: 1/20-1/50. Western Blot (Non-Reducing Conditions). Detects a band of approximately 62kDa in Colo 320HSR cell lysates. Flow Cytometry: Use 10 µl of Neat antibody to label 1x10e6 cells in 100 µl. Immunohistochemistry on Frozen and Paraffin Sections: This product does not require protein digestion pre-treatment of paraffin sections prior to staining. It does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
Background	p62c-myc is involved in the regulation of the cell cycle and cell growth. p62c-myc is primarily located to the cell nucleus, but has also been shown to localised to the cytoplasm in several cell lines. Overexpression of c-myc has been reported in a wide variety of human cancers.
Concentration	0.1 mg/ml
General Readings	Evan GI, Lewis GK, Ramsay G, Bishop JM. Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product. Mol Cell Biol. 1985 Dec;5(12):3610-6. PubMed PMID: 3915782. Spandidos DA, Pintzas A, Kakkanas A, Yiagnisis M, Mahera H, Patra E, et al. Elevated expression of the myc gene in human benign and malignant breast lesions compared to normal tissue. Anticancer Res. 1987 Nov-Dec;7(6):1299-304. PubMed PMID: 3327455.
Storage	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Format	Label: Biotin Purification: Affinity Chromatography on Protein G. Buffer System: PBS, pH 7.4 containing 0.09% Sodium Azide as preservative and 1% BSA as stabilizer. State: Liquid purified Ig fraction.
Species Reactivity	Species reactivity (tested): Human.
Specificity	Specificity: This antibody detects the 62kDa c-myc gene product. This antibody may also be used to detect the commonly used c-myc tag.
Gene ID	4609
Targets	c-Myc antibody

Accessory Products

Proteins and/or Positive Controls

Proteins for c-Myc Epitope Tag (EQKLISEEDL) (4 products)

Catalog No.	Species	Pres.	Source
H00004609-P01-10	c-Myc
	Human	Purified		10 µg / €350.00
				Abnova Taiwan Corp.
H00004609-P01-25	c-Myc
	Human	Purified		25 µg / €500.00
				Abnova Taiwan Corp.
H00004609-Q01-10	c-Myc
	Human		in vitro transl.	10 µg / €350.00
				Abnova Taiwan Corp.
H00004609-Q01-25	c-Myc
	Human		in vitro transl.	25 µg / €500.00
				Abnova Taiwan Corp.

Positive controls for c-Myc Epitope Tag (EQKLISEEDL) (1 products)

Catalog No.	Species
NBL1-13414	c-Myc Lysate
		0.1 mg / €370.00
		Novus Biologicals Inc.

Recommended Secondary Antibodies for c-Myc Epitope Tag (EQKLISEEDL) (8 products)

Catalog No.	Host	Pres.
R1253AP	Mouse IgG (H+L chain) antibody
	Rabbit	AP
			Acris Antibodies GmbH
R1253B	Mouse IgG (H+L chain) antibody
	Rabbit	Biotin
			Acris Antibodies GmbH
R1253F	Mouse IgG (H+L chain) antibody
	Rabbit	FITC
			Acris Antibodies GmbH
R1253HRP	Mouse IgG (H+L chain) antibody
	Rabbit	HRP
			Acris Antibodies GmbH
R1253TR	Mouse IgG (H+L chain) antibody
	Rabbit	Texas Red
			Acris Antibodies GmbH
R1403R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Rabbit	PE
			Acris Antibodies GmbH
R1405R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Goat	PE
			Acris Antibodies GmbH
R1455R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Donkey	PE
			Acris Antibodies GmbH