SM1863PX c-Myc Epitope Tag (EQKLISEEDL) antibody

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2 mg / €1,240.00
Please visit the appropriate website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti Human c-Myc Epitope Tag (EQKLISEEDL) 9E10

SM1863PX

Product Description for c-Myc Epitope Tag (EQKLISEEDL)

Mouse anti Human c-Myc Epitope Tag (EQKLISEEDL) 9E10.
Presentation: Purified
Product is tested for Frozen Sections, Enzyme Immunoassay, Western blot / Immunoblot, Flow Cytometry, Paraffin Sections.

Properties for c-Myc Epitope Tag (EQKLISEEDL)

Product Category Antibodies
Target Category
Quantity 2 mg
Synonyms c-myc tag, myc tag, myc-tag
Presentation Purified
Reactivity Hu
Applications C, E, F, P, WB
Clonality Monoclonal
Clone 9E10
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P01106
Immunogen:
Synthetic peptide sequence AEEQKLISEEDLL corresponding to the C-terminal region of Human c-Myc.
Spleen cells from immunised Balb/c mice were fused with cells of the myeloma cell line.
Genename:
4609
Antibody type Ab
Isotype control AM03095PU-N, SM10P (for use in human samples)
Application ELISA: 1/100-1/500.
Western blot: 1/100-1/500 (Non-Reducing Conditions).
Detects a band of approximately 62kDa in Colo 320HSR cell lysates.
Flow cytometry: Use 10 µl of Neat-1/10 diluted antibody to label 1x10e6 cells in 100 µl.
Immunohistochemistry on Frozen and Paraffin Sections: This product does not require protein digestion pre-treatment of paraffin sections prior to staining. It does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
Background p62c-myc is primarily located to the cell nucleus, but has also been shown to localised to the cytoplasm in several cell lines. Overexpression of c-myc has been reported in a wide variety of human cancers.
Concentration 1.0 mg/ml
General Readings
  1. Evan GI, Lewis GK, Ramsay G, Bishop JM. Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product. Mol Cell Biol. 1985 Dec;5(12):3610-6. PubMed PMID: 3915782. (Free PMC Article available)
  2. Spandidos DA, Pintzas A, Kakkanas A, Yiagnisis M, Mahera H, Patra E, et al. Elevated expression of the myc gene in human benign and malignant breast lesions compared to normal tissue. Anticancer Res. 1987 Nov-Dec;7(6):1299-304. PubMed PMID: 3327455.
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Affinity Chromatography on Protein G
Buffer System:
PBS, pH 7.4 containing 0.09% Sodium Azide as preservative
State:
Liquid purified IgG fraction
Purified
Specificity
Specificity:
This antibody detects the 62kDa c-myc gene product, which is involved in the regulation of the cell cycle and cell growth.
This antibody may also be used to detect the commonly used c-Myc 'Tag'.
Species:
Human.
Gene ID 4609

Accessory Products

Proteins and/or Positive Controls

Proteins for c-Myc Epitope Tag (EQKLISEEDL) (5 products)

Catalog No. Species Pres. Purity   Source  
GTX48176-PRO

c-Myc

c-Myc E. coli Aff - Purified Purified by nickel NTA affinity chromatography
50 µg / €670.00
  GeneTex Inc.
H00004609-P01-10

c-Myc

c-Myc Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €350.00
  Abnova Taiwan Corp.
H00004609-P01-25

c-Myc

c-Myc Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €500.00
  Abnova Taiwan Corp.
H00004609-Q01-10

c-Myc

c-Myc Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €350.00
  Abnova Taiwan Corp.
H00004609-Q01-25

c-Myc

c-Myc Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €500.00
  Abnova Taiwan Corp.

Positive controls for c-Myc Epitope Tag (EQKLISEEDL) (1 products)

Catalog No. Species Pres. Purity   Source  
NBL1-13414

c-Myc Lysate

Western Blot: c-Myc Lysate [NBL1-13414] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for MYC
0.1 mg / €370.00
  Novus Biologicals Inc.
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