Caspase-4 (N-term) - SP2145P | acris-antibodies.com

JavaScript seems to be disabled in your browser.
You must have JavaScript enabled in your browser to utilize the functionality of this website.

Home » Antibodies » Caspase-4

Product Description for Caspase-4

Rabbit anti Human, Mouse Caspase-4.
Properties: (N-term)
Presentation: Purified
Product is tested for Western blot / Immunoblot, Paraffin Sections.

Properties for Caspase-4

Product Category	Antibodies
Quantity	0.1 mg
Synonyms	CASP4, ICE(rel)-II, ICH2, Protease ICH-2, Protease TX
Presentation	Purified
Reactivity	Hu, Ms
Applications	P, WB
Clonality	Polyclonal
Host	Rabbit
Isotype	IgG
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	Acris Antibodies GmbH

Datasheet Extract

Immunogen	Swiss Prot Num: P49662 Immunogen: A 16 amino acid peptide from Human Caspase-4 amino terminus Genename: 837
Property	N-term
Antibody type	Ab
Application	Western blot: 1.0 µg/ml. Immunohistochemistry on paraffin sections: 2.0 µg/ml. This product does not require protein digestion pre-treatment of paraffin embedded sections e.g. trypsin or pronase prior to staining. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections. Recommended Positive Control: Spleen tissue.
Background	Caspase-4, a member of the Caspase-1 subfamily of cysteine proteases, exists as an inactive pro-enzyme which undergoes proteolytic cleavage of its p30 precursor form, into smaller p20 and p10 subunits. The cellular localisation of Caspase-4 on the Endoplasmic Reticulum (ER) membrane, has resulted in this protein becoming a focus of studies in which dysfunction or stress to the ER membrane is implicated, such as Alzheimers disease and Ischemia (1, 3) and confirms the involvement of Caspase-4 as an instigator of cellular apoptosis.
Concentration	1.0 mg/ml
General Readings	Hitomi, J. et al. (2004) Involvement of caspase-4 in endoplasmic reticulum stress-induced apoptosis and A beta-induced cell death. J. Cell Biol. 165: 347-236. Kamens, J. et al. (1995) Identification and characterization of ICH-2, a novel member of the Interleukin-1Betaconverting enzyme family of cysteine proteases. J. Biol. Chem. 270: 15250-15256. Katayama, T. et al. (2004) Induction of neuronal death by ER stress in Alzheimers disease. J. Chem. Neuroanat. 28(1-2): 67-78. Cohen, G.M. (1997) Caspases: the executioners of apoptosis. Biochem. J. 326: 1-16.
Storage	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Format	Purification: Affinity Chromatography Buffer System: PBS Preservatives: 0.02% Sodium Azide State: Liquid purified IgG fraction Purified
Specificity	Specificity: This antibody recognises an epitope within the N-terminal region (NT) of Caspase-4, otherwise known as ICH-2. This antibody detects a band of approximately 34 kDa in Ramos cell lysates (predicted precursor MWT 31 kDa). Species: Human, Mouse.
Gene ID	837
FocusOn and Reviews	FocusOns: Antibodies to Caspase Family - FocusOn 051
Targets	Caspase-4 antibody

Accessory Products

Proteins and/or Positive Controls

Proteins for Caspase-4 (2 products)

Catalog No.	Species	Pres.	Purity		Source
H00000837-P01-10	Caspase-4
	Human	Purified					10 µg / €350.00
							Abnova Taiwan Corp.
H00000837-P01-25	Caspase-4
	Human	Purified					25 µg / €500.00
							Abnova Taiwan Corp.

Positive controls for Caspase-4 (2 products)

Catalog No.	Species	Pres.	Purity		Source
NBL1-08712	Caspase 4 Lysate
							0.1 mg / €370.00
							Novus Biologicals Inc.
NBL1-08713	Caspase 4 Lysate
							0.1 mg / €370.00
							Novus Biologicals Inc.

Recommended Secondary Antibodies for Caspase-4 (6 products)

Catalog No.	Host	Clone/Iso.	Pres.		React.	Applications
R1364AP	Rabbit IgG (H+L chain) antibody
	Goat		AP
							Acris Antibodies GmbH
R1364B	Rabbit IgG (H+L chain) antibody
	Goat		Biotin
							Acris Antibodies GmbH
R1364F	Rabbit IgG (H+L chain) antibody
	Goat		FITC
							Acris Antibodies GmbH
R1364HRP	Rabbit IgG (H+L chain) antibody
	Goat		HRP
							Acris Antibodies GmbH
R1364T	Rabbit IgG (H+L chain) antibody
	Goat		TRITC
							Acris Antibodies GmbH
R1427R	Rabbit IgG F(ab)2 Fragment (H+L chain) antibody
	Donkey		PE
							Acris Antibodies GmbH

Click here to see all secondary antibodies for Caspase-4

Service

Acris Antibodies GmbH
Schillerstraße 5
D-32052 Herford
Germany
phone: +49-5221-34606-0
free call: 0800-227 47 46 (Germany only)
fax: +49-5221-34606-11
email: info@acris-online.de
orders: ordering@acris-online.de

© Copyright 1998 - 2013 Acris Antibodies GmbH