Caspase-7 (Active Form) - AM08364PU-N

Product Description for Caspase-7

Mouse anti Human Caspase-7 25B881.1.
Properties: (Active Form)
Presentation: Purified
Product is tested for Paraffin Sections, Western blot / Immunoblot.

Properties for Caspase-7

Product Category	Antibodies
Quantity	50 µg
Synonyms	Apoptotic protease Mch-3, CASP-7, CASP7, CMH-1, FocusOn051, ICE-like apoptotic protease 3, MCH3
Presentation	Purified
Reactivity	Hu
Applications	P, WB
Clonality	Monoclonal
Clone	25B881.1
Host	Mouse
Isotype	IgG1
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	Acris Antibodies GmbH

Datasheet Extract

Immunogen	Swiss Prot Num: P55210 Immunogen: Recombinant protein of Active form of Human Caspase-7. Genename: 840
Property	Active Form
Antibody type	Ab
Application	Western blot: 1-2 µg/ml. Immunohistochemistry on Paraffin Sections: 10 µg/ml. Heat induced antigen retrieval in pH 6.0 citrate buffer is recommended.
Background	Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. The precursor form of all caspases is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into a2b2 tetramer to form the active enzyme. Caspase-7 is derived from the cleavage of proMCH3-alpha into 2 subunits, p20 and p12 by Caspase-3. Sequence analysis revealed that the MCH3-alpha amino acid sequence had the highest homology to caspase-3 (53% identity) and less than 37% identity with all other family members.
Concentration	0.5 mg/ml
Storage	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Format	Purification: Affinity Chromatography on Protein G Buffer System: PBS containing 0.2% Gelatin and 0.05% Sodium Azide as preservative State: Liquid purified Ig fraction Purified
Specificity	Specificity: Recognizes Human Caspase 7 (CASP7).
Gene ID	840
FocusOn and Reviews	FocusOns: Antibodies to Caspase Family - FocusOn 051
Targets	Caspase-7 antibody

Accessory Products

Proteins and/or Positive Controls

Proteins for Caspase-7 (5 products)

Catalog No.	Species	Pres.	Source
H00000840-Q01-10	Caspase-7
	Human		in vitro transl.	10 µg / €350.00
				Abnova Taiwan Corp.
H00000840-Q01-25	Caspase-7
	Human		in vitro transl.	25 µg / €500.00
				Abnova Taiwan Corp.
H00000840-Q02	Caspase-7
	Human	Purified		10 µg / €330.00
				Abnova Taiwan Corp.
H00000840-Q02-10	Caspase-7
	Human		in vitro transl.	10 µg / €350.00
				Abnova Taiwan Corp.
H00000840-Q02-25	Caspase-7
	Human		in vitro transl.	25 µg / €500.00
				Abnova Taiwan Corp.

Positive controls for Caspase-7 (2 products)

Catalog No.	Species
H00000840-T01	CASP7 293T Cell Transient Overexpression Lysate(Denatured)
		0.1 ml / €240.00
		Abnova Taiwan Corp.
NBL1-08716	Caspase 7 Lysate
		0.1 mg / €370.00
		Novus Biologicals Inc.

Recommended Secondary Antibodies for Caspase-7 (8 products)

Catalog No.	Host	Pres.
R1253AP	Mouse IgG (H+L chain) antibody
	Rabbit	AP
			Acris Antibodies GmbH
R1253B	Mouse IgG (H+L chain) antibody
	Rabbit	Biotin
			Acris Antibodies GmbH
R1253F	Mouse IgG (H+L chain) antibody
	Rabbit	FITC
			Acris Antibodies GmbH
R1253HRP	Mouse IgG (H+L chain) antibody
	Rabbit	HRP
			Acris Antibodies GmbH
R1253TR	Mouse IgG (H+L chain) antibody
	Rabbit	Texas Red
			Acris Antibodies GmbH
R1403R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Rabbit	PE
			Acris Antibodies GmbH
R1405R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Goat	PE
			Acris Antibodies GmbH
R1455R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Donkey	PE
			Acris Antibodies GmbH