Product Description for Caspase-9
Rabbit anti Bovine, Human, Mouse, Rat, Sheep Caspase-9.
Presentation: Purified
Product is tested for Immunoprecipitation, Western blot / Immunoblot, Paraffin Sections.
Presentation: Purified
Product is tested for Immunoprecipitation, Western blot / Immunoblot, Paraffin Sections.
Properties for Caspase-9
| Product Category | Antibodies |
|---|---|
| Quantity | 0.5 mg |
| Synonyms | APAF-3, APAF3, Apoptotic protease Mch-6, Apoptotic protease-activating factor 3, CASP-9, CASP9, FocusOn051, ICE-LAP6, ICE-like apoptotic protease 6, MCH6 |
| Presentation | Purified |
| Reactivity | Bov, Hu, Ms, Rt, Sh |
| Applications | IP, P, WB |
| Clonality | Polyclonal |
| Host | Rabbit |
| Shipping to | Worldwide |
| PDF datasheet | View Datasheet |
| Manufacturer | Acris Antibodies GmbH |
Datasheet Extract
| Immunogen |
|
|---|---|
| Antibody type | Ab |
| Application | Western Blotting: Use 2.5-5 µg/ml for 2hrs at RT. Immunoprecipitation: Use 10 µg/mg protein lysate (Denatured verified), Use Protein A. Immunohistology (Formalin/Paraffin): 5-10 µg/ml for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Recommended Positive Control: Jurkat Cells, Tonsil. |
| Background | Activation of procaspase-9 by Apaf-1 in the cytochrome c/ dATP-dependent pathway requires proteolytic cleavage to generate the mature caspase molecule. Deletion of the Apaf-1 WD-40 repeats makes Apaf-1 constitutively active and capable of processing procaspase-9 independent of cytochrome c and dATP. Apaf-1-mediated processing of procaspase-9 occurs at Asp-315 by an intrinsic autocatalytic activity of procaspase-9 itself. Apaf-1 can form oligomers and may facilitate procaspase-9 autoactivation by oligomerizing its precursor molecules. Once activated, Caspase 9 can initiate a caspase cascade involving the downstream executioners Caspase 3, 6, and 7. |
| Concentration | 1.0 mg/ml |
| General Readings |
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| Storage | Store the antibody undiluted at 2-8°C. Shelf life: one year from despatch. |
| Format |
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| Specificity |
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| Gene ID | 842 |
| FocusOn and Reviews | |
| Targets |
Accessory Products
Proteins and/or Positive Controls
Proteins for Caspase-9 (4 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| GTX27885 |
Caspase-9 (41-56) |
||||||
 |
50 µg /
€230.00
|
||||||
| GeneTex Inc. | |||||||
| GTX27886 |
Caspase-9 (299-318) |
||||||
|
50 µg /
€230.00
|
||||||
| GeneTex Inc. | |||||||
| H00000842-P01-10 |
Caspase-9 |
||||||
|
Human | in vitro transl. |
10 µg /
€350.00
|
||||
| Abnova Taiwan Corp. | |||||||
| H00000842-P01-25 |
Caspase-9 |
||||||
|
Human | in vitro transl. |
25 µg /
€500.00
|
||||
| Abnova Taiwan Corp. | |||||||
Recommended Secondary Antibodies for Caspase-9 (6 products)
| Catalog No. | Host | Clone/Iso. | Pres. | React. | Applications | ||
|---|---|---|---|---|---|---|---|
| R1364AP |
Rabbit IgG (H+L chain) antibody |
||||||
|
|
Goat | AP | |||||
| Acris Antibodies GmbH | |||||||
| R1364B |
Rabbit IgG (H+L chain) antibody |
||||||
|
|
Goat | Biotin | |||||
| Acris Antibodies GmbH | |||||||
| R1364F |
Rabbit IgG (H+L chain) antibody |
||||||
|
|
Goat | FITC | |||||
| Acris Antibodies GmbH | |||||||
| R1364HRP |
Rabbit IgG (H+L chain) antibody |
||||||
|
Goat | HRP | |||||
| Acris Antibodies GmbH | |||||||
| R1364T |
Rabbit IgG (H+L chain) antibody |
||||||
|
|
Goat | TRITC | |||||
| Acris Antibodies GmbH | |||||||
| R1427R |
Rabbit IgG F(ab)2 Fragment (H+L chain) antibody |
||||||
|
|
Donkey | PE | |||||
| Acris Antibodies GmbH | |||||||
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