CD163

Product Description for CD163

Mouse anti Human CD163 5C6-FAT.
Presentation: Purified
Product is tested for Frozen Sections, Enzyme Immunoassay, Western blot / Immunoblot, Flow Cytometry, Paraffin Sections.

Properties for CD163

Product Category	Antibodies
Quantity	0.1 mg
Synonyms	FocusOn010, FocusOn027, Hemoglobin scavenger receptor, M130, Macrophage marker, Scavenger receptor cysteine-rich type 1 protein M130
Presentation	Purified
Reactivity	Hu
Applications	C, E, F, P, WB
Clonality	Monoclonal
Clone	5C6-FAT
Host	Mouse
Isotype	IgG1
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	Acris Antibodies GmbH

Datasheet Extract

Immunogen	Swiss Prot Num: Q86VB7 Immunogen: Human CD163. The epitope has not been further characterized. Genename: 9332
Antibody type	Ab
Application	ELISA. Immunohistochemistry on Frozen Sections: 0.5 µg/ml (1/400) Immunohistochemistry on Paraffin Sections: 1 µg/ml (1/200) (Proteinase K pretreatment for antigen retrieval is recommended). Suggested Positive Control: Human placenta. Has been described to work in FACS and Western blots.
Background	CD163 is a scavenger receptor for the haemoglobin-haptoglobin complex, and is upregulated by glucocorticoids and IL-10. The extracellular portion of the receptor is regularly shed and can be found in the circulation. An important function of CD163 seems to be in the adhesion of monocytes to activated endothelial cells. CD163-positive cells include skin histiocytes, Kupffer cells, spleen macrophages of the red pulp, and some thymus macrophages. The antigen is also found abundantly in human term placenta, and regularly in acute and chronic inflammatory lesions.
Concentration	0.2 mg/ml (after reconstitution)
Protocols	Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature 1. Wash in PBS 2. Block endogenous peroxidase 3. Wash in PBS 4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber 5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber 6. Wash in PBS 7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM (H+L) minimal-cross reaction to human) for 1h in a humid chamber 8. Wash in PBS 9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS 11. Counterstain with Mayer’s hemalum. Protocol with formalin-fixed, paraffin-embedded sections: The whole procedure is performed at room temperature 1. Deparaffinize and rehydrate tissue section 2. Incubate the tissue section with proteinase K for 7min. 3. Wash in distilled water 4. Block endogenous peroxidase 5. Wash in PBS 6. Block with 10% normal goat serum in PBS for 30min. in a humid chamber 7. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber 8. Wash in PBS 9. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM (H+L) minimal-cross reaction to human) for 1h in a humid chamber 10. Wash in PBS 11. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 12. Wash in PBS 13. Counterstain with Mayer’s hemalum
Product Citations	Zaba LC, Cardinale I, Gilleaudeau P, Sullivan-Whalen M, Suárez-Fariñas M, Suárez Fariñas M, et al. Amelioration of epidermal hyperplasia by TNF inhibition is associated with reduced Th17 responses. J Exp Med. 2007 Dec 24;204(13):3183-94. Epub 2007 Nov 26. PubMed PMID: 18039949.
General Readings	Hoegger, P. et al.: J. Immunology 161: 1883-1890 (1998)
Storage	Store original vial at 2-8°C. After reconstitution store the antibody undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Format	Purification: Affinity Chromatography Buffer System: Stock Solution contains PBS, pH 7.2 with 5 mg/ml BSA as a stabilizer and 0.05% Kathon as a preservative. State: Lyophilized purified IgG fraction Purified Reconstitution: Restore with 0.5 ml distilled water.
Species Reactivity	Species reactivity (tested): Human.
Specificity	Specificity: Monoclonal antibody 5C6 FAT recognizes a membrane glycoprotein on monocytes and macrophages which is expressed in intermediate and late inflammatory stages. Antigen Distribution Isolated cells: Monocytes, particularly after dexamethasone treatment or after 2-5 days in culture. Does not react with lymphocytes, granulocytes or platelets. Tissue Sections: Positive staining can be observed in the skin (histiocytes), gut, Kupffer cells, few alveolar macrophages, a major population of macrophages in the placenta, varying degrees of macrophages in inflamed tissues, including tumorous tissue depending on the inflammatory stage. Red pulp, but not white pulp macrophages of the spleen, and cortical macrophages of the thymus are detected. Macrophages in the synovialis of patients with rheumatoid arthritis. In alveolar macrophages and in Kupffer cells a double staining can be observed with monoclonal antibody 25F9 (product BM4022) which is not the case in other tissues.
Gene ID	9332
FocusOn and Reviews	FocusOns: CD163: Marker for intermediate and late stage Inflammatory Macrophages - FocusOn 010 Antibodies to Human Macrophages - FocusOn 027
Targets	CD163 antibody

Accessory Products

Proteins and/or Positive Controls

Proteins for CD163 (2 products)

Catalog No.	Species	Source
H00009332-Q01-10	CD163
	Human	in vitro transl.	10 µg / €350.00
			Abnova Taiwan Corp.
H00009332-Q01-25	CD163
	Human	in vitro transl.	25 µg / €500.00
			Abnova Taiwan Corp.

Positive controls for CD163 (1 products)

Catalog No.	Species
NBL1-08909	CD163 Lysate
		0.1 mg / €370.00
		Novus Biologicals Inc.

Recommended Secondary Antibodies for CD163 (8 products)

Catalog No.	Host	Pres.
R1253AP	Mouse IgG (H+L chain) antibody
	Rabbit	AP
			Acris Antibodies GmbH
R1253B	Mouse IgG (H+L chain) antibody
	Rabbit	Biotin
			Acris Antibodies GmbH
R1253F	Mouse IgG (H+L chain) antibody
	Rabbit	FITC
			Acris Antibodies GmbH
R1253HRP	Mouse IgG (H+L chain) antibody
	Rabbit	HRP
			Acris Antibodies GmbH
R1253TR	Mouse IgG (H+L chain) antibody
	Rabbit	Texas Red
			Acris Antibodies GmbH
R1403R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Rabbit	PE
			Acris Antibodies GmbH
R1405R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Goat	PE
			Acris Antibodies GmbH
R1455R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Donkey	PE
			Acris Antibodies GmbH