AM32057SU-N CD243 / MDR1 antibody

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1 ml / €410.00
Please visit the appropriate website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti Hamster, Human CD243 / MDR1 JSB-1

Product Description for CD243 / MDR1

Mouse anti Hamster, Human CD243 / MDR1 JSB-1.
Presentation: Supernatant
Product is tested for Western blot / Immunoblot, Paraffin Sections, Immunocytochemistry/Immunofluorescence, Frozen Sections.

Properties for CD243 / MDR1

Product Category Primary Antibodies
Target Category
Quantity 1 ml
Synonyms ABCB1, ATP-binding cassette sub-family B member 1, Multidrug resistance protein 1, P-glycoprotein 1, PGY1
Presentation Supernatant
Reactivity Hst, Hu
Applications C, ICC/IF, P, WB
Clonality Monoclonal
Clone JSB-1
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P08183
Genename:
5243
Antibody type Ab
Application Western blot: Use an anti-Mouse-HRP.
Immunocytochemistry on Aceton Fixed Cell Preparation: at least 1/20. 
Flow Cytometry: 1/10. Cell permeabilization in 10% (v/v) Lysing solution followed by primairy antibody and anti-Mouse-FITC required. 
Immunohistochemistry on Acetone-Fixed Frozen Sections: 1/20 
Immunohistochemistry on Formalin-Fixed Paraffin Embedded Sections: 1/20.  
Optimal staining results are obtained with routine 2-step ABC or APAAP methods using acetone fixed cytocentrifuge preparations or cryostat sections. 
In case the B-5 fixative is used (See Protocols) also paraffin embedded tissue can be stained with the antibody.
Background P Glycoprotein, the product of the MDR1 gene, is expressed in distinct non-malignant cells, typically cells with secretory and excretory functions. It is assumed to function as an ATP-dependent drug efflux pump with broad substrate specificity. The highest expression of P Glycoprotein has been observed in kidney (proximal tubules), liver (bile canaliculi), adrenal gland and intestine, suggesting that the primary role of P Glycoprotein is in the normal secretion of physiological metabolites and ingested chemicals into bile, urine and the lumen of the intestinal tract. Elevated levels of P Glycoprotein have also been reported in multidrug-resistant cell lines and in colon, endometrial, ovarian, and breast tumors, as well as in sarcomas and leukemias / lymphomas.
Concentration approx. 0.25 mg/ml
Protocols B-5 Fixative:
Fix tissue in freshly prepared B-5 fixative: add 2 ml of 40% formalin to 20 ml of B-5 Stock Solution. Sections must be dezenkerized to remove mercuric pigment before immunostaining. Dezenkerize by placing slides with sections in Gram's Iodine solution for 5 min. at room temperature. Wash well in distilled water. Place slides in Sodium Thiosulphate solution for 5 min. at room temperature. Wash in running tap water for 3 min. Place in PBS for further immunochemical procedures.

B-5 Stock Solution
12 g Mercuric Chloride
2.5 g Sodium Acetate
dissolve in 200 ml distilled water.

Gram's Iodine
1 g I2
2 g KI
dissolve in 300 ml distilled water.

Sodium Thiosulphate solution
15 g Na2S2O3*5H2O
dissolve in 300 ml distilled water.
General Readings
  1. Scheper, R.J., et al., 1986, Int. J. Cancer 42, 389.
  2. Dalton WS, Grogan TM, Meltzer PS, Scheper RJ, Durie BG, Taylor CW, et al. Drug-resistance in multiple myeloma and non-Hodgkin's lymphoma: detection of P-glycoprotein and potential circumvention by addition of verapamil to chemotherapy. J Clin Oncol. 1989 Apr;7(4):415-24. PubMed PMID: 2564428.
  3. Tiirikainen MI, Syrjälä MT, Jansson SE, Krusius T. Flow cytometric analysis of P-glycoprotein in normal and leukemic cells. Ann Hematol. 1992 Sep;65(3):124-30. PubMed PMID: 1356449.
  4. Grogan TM, Spier CM, Salmon SE, Matzner M, Rybski J, Weinstein RS, et al. P-glycoprotein expression in human plasma cell myeloma: correlation with prior chemotherapy. Blood. 1993 Jan 15;81(2):490-5. PubMed PMID: 8093668.
  5. Itsubo M, Ishikawa T, Toda G, Tanaka M. Immunohistochemical study of expression and cellular localization of the multidrug resistance gene product P-glycoprotein in primary liver carcinoma. Cancer. 1994 Jan 15;73(2):298-303. PubMed PMID: 7904895.
  6. Toth, K., et al., 1994, Am. J. Pathol. 144 227.
  7. Gala, et al., 1994, J. Clin. Pathol. 47 619.
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
Format
Preservatives:
0.09% Sodium Azide
Stabilizers:
1% BSA
State:
Liquid Tissue Cell Culture Supernatant
Supernatant
Species Reactivity
Species reactivity (tested):
Human, Chinese Hamster. Neagative with Mouse and Rat
Specificity
Specificity:

The antibody JSB-1 recognizes a strongly a conserved cytoplasmic epitope of the plasma membrane-associated 170-180 kD glycoprotein, the expression of which is strongly correlated with the degree of multi-drug-resistance (MDR) derived MDR cell lines and human MDR cell lines, including cell lines derived from lung, ovaries and B cell lymphomas.

Gene ID 5243

Accessory Products

Proteins and/or Positive Controls

Proteins for CD243 / MDR1 (2 products)

Catalog No. Species Pres. Purity   Source  
H00005243-Q01-10

CD243 / MDR1

CD243 / MDR1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €350.00
  Abnova Taiwan Corp.
H00005243-Q01-25

CD243 / MDR1

CD243 / MDR1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €500.00
  Abnova Taiwan Corp.
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