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SM1677PT CD284 / TLR4 antibody

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25 µg / €150.00
Please visit the appropriate website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti Canine, Guinea Pig, Human, Monkey, Porcine CD284 / TLR4 HTA125

SM1677PT

Product Description for CD284 / TLR4

Mouse anti Canine, Guinea Pig, Human, Monkey, Porcine CD284 / TLR4 HTA125.
Presentation: Purified
Product is tested for Immunoprecipitation, Flow Cytometry.

Properties for CD284 / TLR4

Product Category Antibodies
Quantity 25 µg
Synonyms FocusOn004, Toll-like receptor 4
Presentation Purified
Reactivity Can, GP, Hu, Mky, Por
Applications F, IP
Clonality Monoclonal
Clone HTA125
Host Mouse
Isotype IgG2a
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH

Datasheet Extract

Immunogen
Swiss Prot Num:
O00206
Immunogen:
Ba/F3 cell line expressing TLR4.
Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
Antibody type Ab
Application Flow Cytometry: use 10 µl of 1/10-1/25 diluted antibody to label 10e6 cells or 100 µl whole blood.
Immunoprecipitation.
Background TLR4, also known as CD284, has been demonstrated to act as a receptor for LPS on human monocytes and macrophages. TLR4 signalling of LPS stimulation requires the presence of the MD-2 molecule. TLR4 is weakly expressed by resting cells, but is upregulated following stimulation with LPS.
Concentration 1.0 mg/ml
General Readings
  • Shimazu R, Akashi S, Ogata H, Nagai Y, Fukudome K, Miyake K, et al. MD-2, a molecule that confers lipopolysaccharide responsiveness on Toll-like receptor 4. J Exp Med. 1999 Jun 7;189(11):1777-82. PubMed PMID: 10359581.
  • Jiang Q, Akashi S, Miyake K, Petty HR. Lipopolysaccharide induces physical proximity between CD14 and toll-like receptor 4 (TLR4) prior to nuclear translocation of NF-kappa B. J Immunol. 2000 Oct 1;165(7):3541-4. PubMed PMID: 11034352.
  • Yang S, Tamai R, Akashi S, Takeuchi O, Akira S, Sugawara S, et al. Synergistic effect of muramyldipeptide with lipopolysaccharide or lipoteichoic acid to induce inflammatory cytokines in human monocytic cells in culture. Infect Immun. 2001 Apr;69(4):2045-53. PubMed PMID: 11254557.
  • Triantafilou M, Miyake K, Golenbock DT, Triantafilou K. Mediators of innate immune recognition of bacteria concentrate in lipid rafts and facilitate lipopolysaccharide-induced cell activation. J Cell Sci. 2002 Jun 15;115(Pt 12):2603-11. PubMed PMID: 12045230.
  • Kawahara T, Teshima S, Oka A, Sugiyama T, Kishi K, Rokutan K. Type I Helicobacter pylori lipopolysaccharide stimulates toll-like receptor 4 and activates mitogen oxidase 1 in gastric pit cells. Infect Immun. 2001 Jul;69(7):4382-9. PubMed PMID: 11401977.
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Affinity Chromatography on Protein G
Buffer System:
PBS containing 0.09% Sodium Azide as preservative.
State:
Liquid purified IgG fraction.
Purified
Specificity
Specificity:
This antibody recognises the Toll like receptor 4 (TLR4) cell surface antigen.
This antibody has been demonstrated to block activation of monocytes with LPS. We recommend the use of SM1677LE for this purpose.
Species:
Human, Guinea Pig, Rhesus Monkey and Pig.
FocusOn and Reviews
FocusOns:
Targets

Accessory Products

Proteins and/or Positive Controls

Proteins for CD284 / TLR4 (8 products)

Catalog No. Species Pres. Purity   Source  
H00007099-G01

CD284 / TLR4
CD284 / TLR4 Human
10 µg / €530.00
  Abnova Taiwan Corp.
H00007099-P01-10

CD284 / TLR4
CD284 / TLR4 Human Purified
10 µg / €350.00
  Abnova Taiwan Corp.
H00007099-P01-25

CD284 / TLR4
CD284 / TLR4 Human Purified
25 µg / €500.00
  Abnova Taiwan Corp.
H00007099-Q01-10

CD284 / TLR4
CD284 / TLR4 Human in vitro transl.
10 µg / €350.00
  Abnova Taiwan Corp.
H00007099-Q01-25

CD284 / TLR4
CD284 / TLR4 Human in vitro transl.
25 µg / €500.00
  Abnova Taiwan Corp.
H00007099-Q02

CD284 / TLR4
CD284 / TLR4 Human Purified
10 µg / €330.00
  Abnova Taiwan Corp.
H00007099-Q02-10

CD284 / TLR4
CD284 / TLR4 Human Purified
10 µg / €350.00
  Abnova Taiwan Corp.
H00007099-Q02-25

CD284 / TLR4
CD284 / TLR4 Human Purified
25 µg / €500.00
  Abnova Taiwan Corp.

Positive controls for CD284 / TLR4 (1 products)

Catalog No. Species Pres. Purity   Source  
H00007099-T01

CD284 / TLR4
CD284 / TLR4
0.1 ml / €240.00
  Abnova Taiwan Corp.

Recommended Secondary Antibodies for CD284 / TLR4 (8 products)

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