CD289 / TLR9 (268-284) - SM7105P | acris-antibodies.com

Product Description for CD289 / TLR9

Mouse anti Canine, Equine, Human, Monkey, Mouse, Rat CD289 / TLR9 26C593.2.
Properties: (268-284)
Presentation: Purified
Product is tested for Frozen Sections, Electrophoretic Mobility Shift Assay, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot, Flow Cytometry, Paraffin Sections.

Properties for CD289 / TLR9

Product Category	Antibodies
Quantity	0.1 mg
Synonyms	FocusOn004, Toll-like receptor 9, UNQ5798/PRO19605
Presentation	Purified
Reactivity	Can, Eq, Hu, Mky, Ms, Rt
Applications	C, EMSA, F, ICC/IF, P, WB
Clonality	Monoclonal
Clone	26C593.2
Host	Mouse
Isotype	IgG1
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	Acris Antibodies GmbH

Datasheet Extract

Immunogen	Swiss Prot Num: Q9NR96 Immunogen: KLH-conjugated synthetic peptide corresponding to amino acids 268-284 of Human TRL9 isoform A Genename: 54106 Remarks: Genbank accession no. AAF78037.
Property	268-284
Antibody type	Ab
Application	EMSA: See Kindrachuk et al. (2008). Confocal Microscopy: See Greene et al. (2005) Western blotting: 1-5 µg/ml. Human Peripheral Blood Mononuclear Cells (PBMC) at 3 µg/ml or Human intestine cell lysate (use at 6 µg/ml) can be used as a positive control. A protein of approximately 120 kDa is detected. A smaller isoform, TLR9 isoform B (Genbank accession no. AAF72190) containing 975 amino acids may also be observed in some cases. Flow Cytometry (Intracellular): 0.1-2 µg/10e6 cells. Use Ramos cells as positive control. Flow Cytometry (Cell Surface): See Lee et al. (2006) Immunohistochemistry on Frozen Tissues: 10-20 µg/ml (See Miller et al, 2005). Immunohistochemistry on Paraffin Sections: 5 µg/ml. See also Martin-Armas et al. (2005). Immunocytochemistry/Immunofluorescence: See Greene et al. (2005) and Tabeta et al. (2006).
Background	TLR9 is a member of the Toll like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. The gene encoding this protein is predominantly expressed in lung, placenta, and spleen, and lies in close proximity to another family member, TLR8, on chromosome X.
Concentration	0.5 mg/ml
General Readings	Clone citations: Maintenance of colonic homeostasis by distinctive apical TLR9 signaling in intestinal epithelial cells. Lee J., J-H Mo, K. Katakura, I. Alkalay, A. N. Rucker, Y-T. Liu, H-K. Lee, C. Shen, G. Cojocaru, S. Shenouda, M. Kagnoff, L. Eckmann, Y. Ben-Neriah, and E. Raz., Nature Cell Biol., 8, doi:10.1038/ncb1500 (2006). TGF-a Regulates TLR Expression and Function on Epidermal Keratinocytes. Miller LS, OE Sorensen, PT Liu, HR Jalian, D Eshtiaghpour, BE Behmanesh, W Chung, TD Starner, J Kim,, PA. Sieling, T Ganz and RL Modlin. J. Immunol, 174: 6137-6143 (2005). Toll like receptor 9 (TLR9) is present in murine liver sinusoidal endothelial cells (LSECs) and mediates the effect of CpG-oligonucleotides. Martin-Armas M., J. Simon-Santamaria, I. Pettersen, U. Moens, B. Smedsrod and B.R. Sveinbjornsson. Journal of Hepatology, Available online 7 November 2005. TLR-Induced Inflammation in Cystic Fibrosis and Non-Cystic Fibrosis Airway Epithelial Cells. Greene C.M., T. P. Carroll, S. G. J. Smith, C. C. Taggart, J. Devaney, S. Griffin, S. J. O'Neill, and N. G. McElvaney. J. Immunol., 174: 1638-1646 (2005). The Unc93b1 mutation 3d disrupts exogenous antigen presentation and signaling via Toll-like receptors 3, 7 and 9. Koichi Tabeta, Kasper Hoebe, Edith M Janssen, Xin Du, Philippe Georgel, Karine Crozat, Suzanne Mudd, Navjiwan Mann, Sosathya Sovath, Jason Goode, Louis Shamel, Anat A. Nature Immunology 7, 156-164 (2006). General reading: 1. Muzio M, Natoli G, Saccani S, Levrero M, and Mantovani A. J. exp. Med. 187: 2097-2101 (1998). 2. Medzhitov R and Janeway CA. Cell 91: 295-298 (1997). 3. Chuang TH and Ulevitch RJ. Biochim. Biophys. Acta 1518 (1-2):157-161 (2001) 4. Takeuchi O, Kawai T, Sanjo H, Copeland NG, Gilbert DJ, Jenkins NA, Takeda K, and Akira S. Gene 231: 59-65 (1999). 5. Hemmi H, Takeuchi O, Kawai T, Kaisho T, Sato S, Sanjo H, Matsumoto M, Hoshino K, Wagner H, Takeda K, and Shizo A. Nature 408: 740-745 (2000).
Storage	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Format	Purification: Protein G Chromatography. Buffer System: PBS containing 0.05% BSA as stabilizer and 0.05% Sodium Azide as preservative. State: Liquid purified Ig fraction. Purified
Specificity	Specificity: This antibody detects Toll-Like Receptor 9 (TLR9). Species: Human, Canine (Dog), Horse, Mouse, Rat and Rhesus Monkey.
Gene ID	54106
FocusOn and Reviews	FocusOns: Antibodies to Toll-Like Receptor (TLR) Family - FocusOn 004
Targets	CD289 / TLR9 antibody

Accessory Products

Proteins and/or Positive Controls

Proteins for CD289 / TLR9 (1 products)

Catalog No.	Species
H00054106-G01	CD289 / TLR9
	Human	10 µg / €530.00
		Abnova Taiwan Corp.

Positive controls for CD289 / TLR9 (1 products)

Catalog No.	Species
NBL1-16957	TLR9 Lysate
		0.1 mg / €370.00
		Novus Biologicals Inc.

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