AP02384PU-N CD309 / VEGFR-2 / Flk-1 pTyr951 antibody

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0.1 mg / €320.00
Please visit the appropriate website of Acris Antibodies or contact your local Distributor to buy this product.

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Rabbit anti Human, Mouse, Rat CD309 / VEGFR-2 / Flk-1

AP02384PU-N

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Product Description for CD309 / VEGFR-2 / Flk-1

Rabbit anti Human, Mouse, Rat CD309 / VEGFR-2 / Flk-1.
Properties: pTyr951
Presentation: Aff - Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot, Paraffin Sections.

Properties for CD309 / VEGFR-2 / Flk-1

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms FLK1, KDR, Kinase NYK, Kinase insert domain receptor, Protein-tyrosine kinase receptor Flk-1, VEGF Receptor 2, VEGFR2, Vascular endothelial growth factor receptor 2
Presentation Aff - Purified
Reactivity Hu, Ms, Rt
Applications ICC/IF, P, WB
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P35968
Immunogen:
Peptide sequence around the phosphorylation site of Tyrosine 951 (K-D-Yp-V-G).
Genename:
3791
Property pTyr951
Application Western Blot: 1/500-1/1000.
Immunofluorescence: 1/100-1/200.
Immunohistochemistry on Paraffin Embedded Tissue: 1/50-1/100.
Background VEGF receptor 2 is a member of a receptor tyrosine kinase family whose activation plays an essential role in a large number of biological processes such as embryonic development, wound healing, cell proliferation, migration and differentiation. Like other growth factor receptors, upon ligand binding VEGF receptor 2 dimerises and is autophosphorylated on multiple tyrosine residues. These sites can be involved in the regulation of kinase activity or serve as binding sites for SH2 and phosphotyrosine binding containing signalling proteins. Phosphorylation of Tyrosines 1054 and 1059 in the activation loop is required for activation of VEGF receptor 2 and its intrinsic tyrosine kinase activity. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
Concentration 1.0 mg/ml
General Readings
  1. Zeng H, et al. (2001) J Biol Chem. 276(35): 32714-32719.
  2. Dougher M, et al. (1999) Oncogene. 18(8): 1619-1627.
Storage Store the antibody (in aliquots) at -20°C.
Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
Format
Purification:
Affinity Chromatography using epitope-specific immunogen.
Buffer System:
PBS (without Mg2+ and Ca2+), pH 7.4, containing 150 mM NaCl, 0.02% Sodium Azide and 50% glycerol.
State:
Liquid purified IgG fraction.
Aff - Purified
Specificity
Specificity:
The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
This antibody detects endogenous levels of VEGFR2 only when phosphorylated at Tyrosine 951.
Species:
Human, Rat and Mouse.
Gene ID 3791

Accessory Products

Proteins and/or Positive Controls

Proteins for CD309 / VEGFR-2 / Flk-1 (8 products)

Catalog No. Species Pres. Purity   Source  
AR26018PU-L

CD309 / VEGFR-2 / Flk-1 (esKDR)

SDS-PAGE analysis of recombinant endogenous esKDR produced in insect cells. Sample was loaded in 10% SDS-polyacrylamide gel under reducing conditions and stained with Silver staining. Human Purified > 95 % by SDS-PAGE and silver stain Insect cells
20 µg / €430.00
  Acris Antibodies GmbH
AR26018PU-N

CD309 / VEGFR-2 / Flk-1 (esKDR)

SDS-PAGE analysis of recombinant endogenous esKDR produced in insect cells. Sample was loaded in 10% SDS-polyacrylamide gel under reducing conditions and stained with Silver staining. Human Purified > 95 % by SDS-PAGE and silver stain Insect cells
5 µg / €210.00
  Acris Antibodies GmbH
DA3530

CD309 / VEGFR-2 / Flk-1 (D1-D7)

Primary human umbilical vein endothelial (HUVE) cells were stimulated with 10ng/ml VEGF165 which was preincubated with an 100-fold excess of recombinant human soluble VEGFR-2/KDR receptors. Stimulation with bFGF (basic) was used a second control. Human Purified > 95 % pure as by SDS-PAGE and silver stain. Insect cells
5 µg / €130.00
  Acris Antibodies GmbH
DA3530X

CD309 / VEGFR-2 / Flk-1 (D1-D7)

Primary human umbilical vein endothelial (HUVE) cells were stimulated with 10ng/ml VEGF165 which was preincubated with an 100-fold excess of recombinant human soluble VEGFR-2/KDR receptors. Stimulation with bFGF (basic) was used a second control. Human Purified > 90 % pure as by SDS-PAGE and silver stain. Insect cells
50 µg / €360.00
  Acris Antibodies GmbH
DA3531

CD309 / VEGFR-2 / Flk-1 (D1-7) (Fc Chimera)

Inhibition of the VEGF165-induced proliferation of HUVE cells by recombinant human and mouse sKDR(D7)-Fc and sFlk-1(D7)-Fc. HUVECs were stimulated with 10 ng/ml VEGF165, both soluble receptors were added with a 100X excess. Human Purified > 90 % by SDS-PAGE and visualised by silver stain. Insect cells
50 µg / €280.00
  Acris Antibodies GmbH
DA3531S

CD309 / VEGFR-2 / Flk-1 (D1-7) (Fc Chimera)

Inhibition of the VEGF165-induced proliferation of HUVE cells by recombinant human and mouse sKDR(D7)-Fc and sFlk-1(D7)-Fc. HUVECs were stimulated with 10 ng/ml VEGF165, both soluble receptors were added with a 100X excess. Human Purified > 90 % by SDS-PAGE and visualised by silver stain. Insect cells
10 µg / €150.00
  Acris Antibodies GmbH
H00003791-Q01-10

CD309 / VEGFR-2 / Flk-1

CD309 / VEGFR-2 / Flk-1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €350.00
  Abnova Taiwan Corp.
H00003791-Q01-25

CD309 / VEGFR-2 / Flk-1

CD309 / VEGFR-2 / Flk-1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €500.00
  Abnova Taiwan Corp.

Positive controls for CD309 / VEGFR-2 / Flk-1 (1 products)

Catalog No. Species Pres. Purity   Source  
NBL1-06701

VEGF Receptor 2 Lysate

Western Blot: VEGFR1/VEGFR2 Antibody [NB100-527] - Western Blot on VEGFR2 overexpression lysates.
0.1 ml / €170.00
  Novus Biologicals Inc.
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