CD326 / EPCAM / TACSTD1

Product Description for CD326 / EPCAM / TACSTD1

Mouse anti Human CD326 / EPCAM / TACSTD1 MOC-31.
Presentation: Supernatant
Product is tested for Frozen Sections, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot, Paraffin Sections.

Properties for CD326 / EPCAM / TACSTD1

Product Category	Antibodies
Quantity	1 ml
Synonyms	Adenocarcinoma-associated antigen, EGP314, Ep-CAM, Epithelial cell adhesion molecule, Epithelial glycoprotein 314, FocusOn125, GA733-2, KS 1/4 antigen, KSA, M1S2, M4S1, MIC18, Major gastrointestinal tumor-associated protein GA733-2, TROP1, Trop-1, Tumor-associated calcium signal transducer 1
Presentation	Supernatant
Reactivity	Hu
Applications	C, ICC/IF, P, WB
Clonality	Monoclonal
Clone	MOC-31
Host	Mouse
Isotype	IgG1
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	Acris Antibodies GmbH

Datasheet Extract

Immunogen	Swiss Prot Num: P16422 Immunogen: Isolated from small cell lung carcinoma-derived cell line. Genename: 4072
Antibody type	Ab
Application	Immunoblotting. Cell Suspensions. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections (after microwave treatment). Working Dilution: 1/5-1/10. Incubation Time: 1 h at RT. Dilution Buffer: Dilute immediately before use with PBS or TBS. Recommended Positive Control: Bronchial tissue.
Background	Epithelial Cell Adhesion Molecule (EpCAM) is a 40 kDa cell surface antigen. This antigen has been identified independently by a number of groups, and has been known by a variety of names. Several monoclonal antibodies have been raised against EpCAM, many of which have been described as tumour specific molecules on carcinomas. EpCAM is a Type 1 transmembrane glycoprotein. It is expressed on the basolateral membrane of cells by the majority of epithelial tissues, with the exception of adult squamous epithelium and some specific epithelial cell types including hepatocytes and gastric epithelial cells. EpCAM expression has been reported to be a possible marker of early malignancy, with expression being increased in tumour cells, and de novo expression being seen in dysplastic squamous epithelium.
General Readings	Berendsen HH, de Leij L, Postmus PE, Ter Haar JG, Poppema S, The TH. Detection of small cell lung cancer metastases in bone marrow aspirates using monoclonal antibody directed against neuroendocrine differentiation antigen. J Clin Pathol. 1988 Mar;41(3):273-6. PubMed PMID: 2834417. Ruitenbeek T, Gouw AS, Poppema S. Immunocytology of body cavity fluids. MOC-31, a monoclonal antibody discriminating between mesothelial and epithelial cells. Arch Pathol Lab Med. 1994 Mar;118(3):265-9. PubMed PMID: 8135629. Politi E, Kandaraki C, Apostolopoulou C, Kyritsi T, Koutselini H. Immunocytochemical panel for distinguishing between carcinoma and reactive mesothelial cells in body cavity fluids. Diagn Cytopathol. 2005 Mar;32(3):151-5. PubMed PMID: 15690338.
Storage	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Format	Preservatives: 0.09% Sodium Azide State: Liquid Tissue Culture Supernatant Supernatant
Species Reactivity	Species reactivity (tested): Human.
Specificity	Specificity: During the First International Workshop on Small Cell Lung Cancer Antigens in London, April 1987, specific antibodies have been catagorized according to their reactivity patterns. MOC-31 belongs to the so-called "*Cluster 2*" reactivity pattern. This antibody reacts in Western blotting with a 40 kDa membrane-associated protein present in most epithelia and in all lung carcinomas. MOC-31 is unreactive with normal and malignant meso-thelium and therefore especially useful for the de-tection of carcinoma cells in ascites or pleural effusions.
Gene ID	4072
FocusOn and Reviews	FocusOns: Antibodies to Epithelial Cell Adhesion Molecule (EpCAM) - FocusOn 125
Targets	CD326 / EPCAM / TACSTD1 antibody

Accessory Products

Proteins and/or Positive Controls

Proteins for CD326 / EPCAM / TACSTD1 (3 products)

Catalog No.	Species	Source
H00004072-G01	CD326 / EPCAM / TACSTD1
	Human		10 µg / €530.00
			Abnova Taiwan Corp.
H00004072-P01-10	CD326 / EPCAM / TACSTD1
	Human	in vitro transl.	10 µg / €350.00
			Abnova Taiwan Corp.
H00004072-P01-25	CD326 / EPCAM / TACSTD1
	Human	in vitro transl.	25 µg / €500.00
			Abnova Taiwan Corp.

Positive controls for CD326 / EPCAM / TACSTD1 (2 products)

Catalog No.	Species
H00004072-T01	TACSTD1 293T Cell Transient Overexpression Lysate(Denatured)
		0.1 ml / €240.00
		Abnova Taiwan Corp.
H00004072-T02	TACSTD1 293T Cell Transient Overexpression Lysate(Denatured)
		0.1 ml / €240.00
		Abnova Taiwan Corp.

Recommended Secondary Antibodies for CD326 / EPCAM / TACSTD1 (8 products)

Catalog No.	Host	Pres.
R1253AP	Mouse IgG (H+L chain) antibody
	Rabbit	AP
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R1253B	Mouse IgG (H+L chain) antibody
	Rabbit	Biotin
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R1253F	Mouse IgG (H+L chain) antibody
	Rabbit	FITC
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R1253HRP	Mouse IgG (H+L chain) antibody
	Rabbit	HRP
			Acris Antibodies GmbH
R1253TR	Mouse IgG (H+L chain) antibody
	Rabbit	Texas Red
			Acris Antibodies GmbH
R1403R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Rabbit	PE
			Acris Antibodies GmbH
R1405R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Goat	PE
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R1455R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Donkey	PE
			Acris Antibodies GmbH