CD33 / SIGLEC3

Product Description for CD33 / SIGLEC3

Mouse anti Human CD33 / SIGLEC3 WM53.
Presentation: Purified
Product is tested for Flow Cytometry, Frozen Sections, Immunoprecipitation, Western blot / Immunoblot.

Properties for CD33 / SIGLEC3

Product Category	Antibodies
Quantity	0.2 mg
Synonyms	Myeloid cell surface antigen CD33, Sialic acid-binding Ig-like lectin 3, Siglec-3, gp67
Presentation	Purified
Reactivity	Hu
Applications	C, F, IP, WB
Clonality	Monoclonal
Clone	WM53
Host	Mouse
Isotype	IgG1
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	Acris Antibodies GmbH

Datasheet Extract

Immunogen	Swiss Prot Num: P20138 Immunogen: Human AML cells Genename: 945
Antibody type	Ab
Application	Flow Cytometry: Use 10 µl of 1/10-1/50 diluted antibody to label 10e6 cells. Western Blotting. Immunoprecipitation. Immunohistochemistry on Frozen Sections: 1/50-1/100 (Overnight incubation recommended). Positive Control Tissue: Lymphatic tissue. Not suitable on Paraffin Embedded Sections.
Background	CD33 is found on granulocyte and macrophage precursors in the bone marrow, but is not on pluripotent stem cells. The protein is also expressed on, and is a useful marker for, peripheral monocytes. It is also useful for distinguishing myelogenous leukaemia cells from lymphoid or erythroid leukaemias.
Concentration	1.0 mg/ml
General Readings	Favaloro, E.J., Bradstock, K.F., Kabral, A., Grimsley, P. and Berndt, M.C. (1987). Characterisation of monoclonal antibodies to the human myeloid-specific antigen 'gp67' (CD-33). Disease Markers 5: 215-225. Favaloro EJ, Bradstock KF, Kabral A, Grimsley P, Zowtyj H, Zola H. Further characterization of human myeloid antigens (gp160,95; gp150; gp67): investigation of epitopic heterogeneity and non-haemopoietic distribution using panels of monoclonal antibodies belonging to CD-11b, CD-13 and CD-33. Br J Haematol. 1988 Jun;69(2):163-71. PubMed PMID: 2455535.
Storage	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Format	Purification: Affinity Chromatography on Protein G. Buffer System: PBS, pH 7.4 containing 0.09% Sodium Azide as preservative. State: Liquid purified IgG fraction. Purified
Specificity	Specificity: Recognizes the CD33 cell surface glycoprotein, a 67kD molecule expressed by monocytes, granulocyte precursors, myeloid progenitor cells and myeloid leukaemias. Species: Human.
Gene ID	945
Targets	CD33 / SIGLEC3 antibody

Accessory Products

Proteins and/or Positive Controls

Proteins for CD33 / SIGLEC3 (2 products)

Catalog No.	Species	Pres.
NBP1-46250	CD33 / SIGLEC3
	Human	Purified	25 µg / €370.00
			Novus Biologicals Inc.
H00000945-G01	CD33 / SIGLEC3
	Human		10 µg / €530.00
			Abnova Taiwan Corp.

Positive controls for CD33 / SIGLEC3 (1 products)

Catalog No.	Species
H00000945-T01	CD33 293T Cell Transient Overexpression Lysate(Denatured)
		0.1 ml / €240.00
		Abnova Taiwan Corp.

Recommended Secondary Antibodies for CD33 / SIGLEC3 (8 products)

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R1253AP	Mouse IgG (H+L chain) antibody
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R1253B	Mouse IgG (H+L chain) antibody
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R1253F	Mouse IgG (H+L chain) antibody
	Rabbit	FITC
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R1253HRP	Mouse IgG (H+L chain) antibody
	Rabbit	HRP
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R1253TR	Mouse IgG (H+L chain) antibody
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R1403R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Rabbit	PE
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R1405R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
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R1455R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Donkey	PE
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