PP1204P1 CXCL16 antibody

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50 µg / €230.00
Delivery Time: 3-5 working days

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Rabbit anti Human CXCL16

PP1204P1

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  • PP1204P1

Product Description for CXCL16

Rabbit anti Human CXCL16.
Presentation: Aff - Purified
Product is tested for Enzyme Immunoassay, Western blot / Immunoblot, Paraffin Sections.

Properties for CXCL16

Product Category Antibodies
Quantity 50 µg
Synonyms C-X-C motif chemokine 16, SCYB16, SR-PSOX, SRPSOX, Scavenger receptor for phosphatidylserine and oxidized low density lipoprotein, Small-inducible cytokine B16, Transmembrane chemokine CXCL16
Presentation Aff - Purified
Reactivity Hu
Applications E, P, WB
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH

Datasheet Extract

Immunogen
Swiss Prot Num:
Q9H2A7
Immunogen:
Highly pure (> 98%) E.coli derived recombinant Human CXCL16
Genename:
58191
Antibody type Ab
Add. information Centrifuge vial prior to opening!
Application Sandwich ELISA: To detect hCXCL16 by Sandwich ELISA (using 100 µl/well antibody solution) a concentration of 0.5-2.0 µg/ml of this antibody is required. This antibody, in conjunction with a Biotinylated anti-Human CXCL16 (cat.no. PP1204B1 or PP1204B2) as a Detection antibody, allows the detection of 0.2-0.4 ng/well of recombinant hCXCL16.
Western Blot: To detect hCXCL16 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. The detection limit for recombinant hCXCL16 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Immunohistochemistry: The antibody stained FFPE Sections of Human colorectal adenocarcinoma tissue. An HRP-labelled polymer detection system was used with a DAB chromogen. Antigen retrieval is not required for these conditions. 
Working Dilution: 0.5 µg/ml, overnight incubation at 4°C.
Background CXCL16 acts as a scavenger receptor on macrophages, which specifically binds to OxLDL (oxidized low density lipoprotein), suggesting that it may be involved in pathophysiology such as atherogenesis (By similarity). Induces a strong chemotactic response. Induces calcium mobilization. Binds to CXCR6/Bonzo. It is a type I membrane protein (Potential). Also exists as a soluble form. Expressed in spleen, lymph nodes, lung, kidney, small intestine and thymus. Weak expression in heart and liver and no expression in brain and bone marrow.
Product Citations
  • Gutwein P, Abdel-Bakky MS, Schramme A, Doberstein K, Kämpfer-Kolb N, Amann K, et al. CXCL16 is expressed in podocytes and acts as a scavenger receptor for oxidized low-density lipoprotein. Am J Pathol. 2009 Jun;174(6):2061-72. doi: 10.2353/ajpath.2009.080960. Epub 2009 May 12. PubMed PMID: 19435795.
General Readings
  • Scholz, F. The Journal of Investigative Dermatology.;127(6):1444-55. (2007)
Storage Prior to reconstitution store at -20°C.
Following reconstitution store undiluted at 2-8°C for two weeks or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Affinity Chromatography employing immobilized hCXCL16 matrix
Buffer System:
PBS, pH 7.2 without preservatives
State:
Lyophilized (sterile filtered) purified Ig fraction
Aff - Purified
Reconstitution:
Restore in sterile water to a concentration of 0.1-1.0 mg/ml
Specificity
Specificity:
This antibody recognizes Human CXCL16. Other species not tested.
Gene ID 58191
Targets

Accessory Products

Proteins and/or Positive Controls

Proteins for CXCL16 (2 products)

Catalog No. Species Pres. Purity   Source  
H00058191-P01-10

CXCL16

CXCL16 Human in vitro transl.
10 µg / €350.00
  Abnova Taiwan Corp.
H00058191-P01-25

CXCL16

CXCL16 Human in vitro transl.
25 µg / €500.00
  Abnova Taiwan Corp.

Positive controls for CXCL16 (1 products)

Catalog No. Species Pres. Purity   Source  
NBL1-09626

CXCL16 Lysate

Western Blot: CXCL16 Lysate [NBL1-09626] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for CXCL16
0.1 mg / €370.00
  Novus Biologicals Inc.