Product Description for Cyclin B1
Rabbit anti Human Cyclin B1.
Properties: pSer126
Presentation: Aff - Purified
Product is tested for Enzyme Immunoassay.
Properties: pSer126
Presentation: Aff - Purified
Product is tested for Enzyme Immunoassay.
Properties for Cyclin B1
| Product Category | Antibodies |
|---|---|
| Quantity | 0.1 mg |
| Synonyms | CCNB, CCNB1, Cyclin-B1, G2/mitotic-specific cyclin-B1, Review 016 |
| Presentation | Aff - Purified |
| Reactivity | Hu |
| Applications | E |
| Clonality | Polyclonal |
| Host | Rabbit |
| Shipping to | Worldwide |
| PDF datasheet | View Datasheet |
| Manufacturer | Acris Antibodies GmbH |
Datasheet Extract
| Immunogen |
|
|---|---|
| Property | pSer126 |
| Antibody type | Ab |
| Add. information | Protein Sequence: Human Cyclin B1, 433 aa, predicted MW 48.3 kDa 1 malrvtrnsk inaenkakin magakrvpta paatskpglr prtalgdign kvseqlqakm 61 pmkkeakpsa tgkvidkklp kplekvpmlv pvpvsepvpe pepepepepv keeklspepi 121 lvdtaspspm etsgcapaee dlcqafsdvi lavndvdaed gadpnlcsey vkdiyaylrq 181 leeeqavrpk yllgrevtgn mrailidwlv qvqmkfrllq etmymtvsii drfmqnncvp 241 kkmlqlvgvt amfiaskyee myppeigdfa fvtdntytkh qirqmemkil ralnfglgrp 301 lplhflrras kigevdveqh tlakylmelt mldydmvhfp psqiaagafc lalkildnge 361 wtptlqhyls yteesllpvm qhlaknvvmv nqgltkhmtv knkyatskha kistlpqlns 421 alvqdlakav akv |
| Application | This affinity purified antibody has been tested for use in ELISA (1:10,000-1:50,000) and Western blot (1:100-1:1,000). Specific conditions for reactivity should be optimized by the end user. Expect a band ~ 48 kDa in size corresponding to Cyclin B1 by western blotting in the appropriate cell lysate or extract. |
| Background | Cyclin B1 (also called CCNB1 and G2/mitotic-specific cyclin B1) is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form a serine/threonine kinase holoenzyme complex called the maturation-promoting factor (MPF). Two alternative transcripts have been found a constitutively expressed transcript and a cell cycle-regulated transcript that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. S126 phosphorylation, the site of phosphorylation recognized by this antibody, is involved in an auto-phosphorylation of the cdk -cyclin complex. Recognition of this phosphorylated site suggests the antibody is useful to detect the active form of the complex and may serve as a marker of mitosis. |
| Concentration | 0.6 mg/ml (by UV absorbance at 280 nm) |
| General Readings |
|
| Storage | Store vial at -20°C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Shelf life: one year from despatch. |
| Format |
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| Specificity |
|
| Gene ID | 891 |
| FocusOn and Reviews | |
| Targets |
Accessory Products
Proteins and/or Positive Controls
Proteins for Cyclin B1 (4 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| GTX28012 |
Cyclin B1 |
||||||
 |
0.1 mg /
€230.00
|
||||||
| GeneTex Inc. | |||||||
| H00000891-P01 |
Cyclin B1 |
||||||
|
Human | Purified |
2 µg /
€250.00
|
||||
| Abnova Taiwan Corp. | |||||||
| H00000891-Q01-10 |
Cyclin B1 |
||||||
|
Human | in vitro transl. |
10 µg /
€350.00
|
||||
| Abnova Taiwan Corp. | |||||||
| H00000891-Q01-25 |
Cyclin B1 |
||||||
|
Human | in vitro transl. |
25 µg /
€500.00
|
||||
| Abnova Taiwan Corp. | |||||||
Recommended Secondary Antibodies for Cyclin B1 (6 products)
| Catalog No. | Host | Clone/Iso. | Pres. | React. | Applications | ||
|---|---|---|---|---|---|---|---|
| R1364AP |
Rabbit IgG (H+L chain) antibody |
||||||
|
|
Goat | AP | |||||
| Acris Antibodies GmbH | |||||||
| R1364B |
Rabbit IgG (H+L chain) antibody |
||||||
|
|
Goat | Biotin | |||||
| Acris Antibodies GmbH | |||||||
| R1364F |
Rabbit IgG (H+L chain) antibody |
||||||
|
|
Goat | FITC | |||||
| Acris Antibodies GmbH | |||||||
| R1364HRP |
Rabbit IgG (H+L chain) antibody |
||||||
|
Goat | HRP | |||||
| Acris Antibodies GmbH | |||||||
| R1364T |
Rabbit IgG (H+L chain) antibody |
||||||
|
|
Goat | TRITC | |||||
| Acris Antibodies GmbH | |||||||
| R1427R |
Rabbit IgG F(ab)2 Fragment (H+L chain) antibody |
||||||
|
|
Donkey | PE | |||||
| Acris Antibodies GmbH | |||||||
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