Cytokeratin 14

Product Description for Cytokeratin 14

Mouse anti Human Cytokeratin 14 LL002.
Presentation: Purified
Product is tested for Frozen Sections, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot, Flow Cytometry, Paraffin Sections.

Properties for Cytokeratin 14

Product Category	Antibodies
Quantity	0.2 mg
Synonyms	CK14, Cytokeratin-14, K14, KRT14, Keratin 14, Keratin Type I Cytoskeletal 14, Review 009
Presentation	Purified
Reactivity	Hu
Applications	C, F, ICC/IF, P, WB
Clonality	Monoclonal
Clone	LL002
Host	Mouse
Isotype	IgG3
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	Acris Antibodies GmbH

Datasheet Extract

Immunogen	Swiss Prot Num: P02533 Immunogen: Last 15 C-terminal residues of Human Cytokeratin 14 conjugated to Thyroglobulin. Genename: 3861
Antibody type	Ab
Application	Western Blot. Immunofluorescence. Flow Cytometry: Use 10 µl of 1/100 diluted antibody to label 10⁶ cells in 100 µl (Membrane permeabilisation is required). Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections: 1/200. This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer is recommended for this purpose. Enzyme digestion is not recommended. Recommended Positive Control: Skin, tonsil or squamous cell carcinoma.
Background	Cytokeratin is a type I intermediate filament, expressed by stratifying epithelial cells and can be used to distinguish these cell types from simple epithelial cells, which do not express cytokeratin 14. Keratin 14 is a member of the keratin family, the most diverse group of intermediate filaments. It is usually found as a heterotetramer with two molecules of keratin 5, a type II keratin. Together they form the cytoskeleton of epithelial cells. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex. Keratin 14 has been studied as a prognostic marker in breast cancer.
Concentration	1.0 mg/ml
General Readings	1. Purkis, P.E. et al. (1990) Antibody markers of basal cells in complex epithelia. J. Cell Sci. 97: 39-50. 2. Lane, E.B. and Alexander, C.M. (1990) Use of keratin antibodies in tumor diagnosis. Semin. Canc. Biol. 1: 165-179. 3. Westzels, R.H.W. et al. (1989) Detection of basement membrane components and basal cell keratin 14 in noninvasive and invasive carcinomas of the breast. Am. J. Pathol. 134: 571-579. 4. Moll, R. et al. (1982) The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells. Cell. 31: 11-24. 5. Varley, C.L. et al. (2004) Activation of peroxisome proliferatoractivated receptorgamma reverses squamous metaplasia and induces transitional differentiation in normal human urothelial cells. Am J Pathol. 164: 178998. 6. Abdeen, S.K. et al. (2011) Wwox inactivation enhances mammary tumorigenesis. Oncogene. 30: 39006. 7. Matos, A.J. et al. (2006) Detection of lymph node micrometastases in malignant mammary tumours in dogs by cytokeratin immunostaining. Vet Rec. 158: 62630. 8. Turner, A. et al. (2011) Transplantation of autologous differentiated urothelium in an experimental model of composite cystoplasty. Eur Urol. 59: 44754. 8. Stumpf, P and Welsch, U. (2004) Secretory and defensive functions of the duct system of the lactating mammary gland of the African elephant (Loxodonta africana, Proboscidea) Zoomorphology 123:155–1679. Alam, H. et al. (2011) Loss of keratins 8 and 18 leads to alterations in {alpha}6{beta}4-integrin-mediated signalling and decreased neoplastic progression in an oral-tumour-derived cell line. J Cell Sci. May 24. [Epub ahead of print] 10. Clark, S.E. et al. (2011) Molecular subtyping of DCIS: heterogeneity of breast cancer reflected in pre-invasive disease. Br J Cancer. 104: 120-7. 11. Hale, L.P. and Markert, M.L. (2004) Corticosteroids regulate epithelial cell differentiation and Hassall body formation in the human thymus. J Immunol. 172: 617-24. 12. Takahashi, C. et al. (2010) Newly established cell lines from mouse oral epithelium regenerate teeth when combined with dental mesenchyme. In Vitro Cell Dev Biol Anim. 46: 457-68. 13. Faustino, A.M. et al. (2007) A salivary malignant myoepithelioma in a dog. Faustino, A.M. and Dias Pereira, P. 14. Collins, A.T. et alet al. (2005) Prospective identification of tumorigenic prostate cancer stem cells. Cancer Res. 65: 10946-51. 15. Varley, C.L. et al. (2004) Activation of peroxisome proliferator-activated receptor-gamma reverses squamous metaplasia and induces transitional differentiation in normal human urothelial cells. Am J Pathol. 164: 1789-98. 16. Abdeen, S.K. et al. (2011) Wwox inactivation enhances mammary tumorigenesis. Oncogene. 30: 3900-6. 17. Matos, A.J. et al. (2006) Detection of lymph node micrometastases in malignant mammary tumours in dogs by cytokeratin immunostaining. Vet Rec. 158: 626-30. 18. Turner, A. et al. (2011) Transplantation of autologous differentiated urothelium in an experimental model of composite cystoplasty. Eur Urol. 59: 447-54. 19. Munz, B. et al. (1999) Overexpression of activin A in the skin of transgenic mice reveals new activities of activin in epidermal morphogenesis, dermal fibrosis and wound repair. EMBO J. 18: 5205-15.
Storage	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Format	Purification: Ion Exchange Chromatography Buffer System: PBS Preservatives: 0.09% Sodium Azide State: Liquid purified IgG fraction Purified
Specificity	Specificity: This antibody recognises Cytokeratin 14, a type I intermediate filament, expressed by stratifying epithelial cells and can be used to distinguish these cell types from simple epithelial cells, which do not express Cytokeratin 14. Species: Human, Elephant, Dog, Pig.
Gene ID	3861
FocusOn and Reviews	Reviews: Antibodies to Cytokeratin Proteins - Product Review 09
Targets	Cytokeratin 14 antibody

Accessory Products

Proteins and/or Positive Controls

Proteins for Cytokeratin 14 (4 products)

Catalog No.	Species	Pres.	Purity	Source
AR09363PU-N	Cytokeratin 14
	Human	Purified	> 95 %		0.25 mg / €630.00
					Acris Antibodies GmbH
AR09363PU-S	Cytokeratin 14
	Human	Purified	> 95 %		0.1 mg / €380.00
					Acris Antibodies GmbH
H00003861-P01-10	Cytokeratin 14
	Human			in vitro transl.	10 µg / €350.00
					Abnova Taiwan Corp.
H00003861-P01-25	Cytokeratin 14
	Human			in vitro transl.	25 µg / €500.00
					Abnova Taiwan Corp.

Positive controls for Cytokeratin 14 (1 products)

Catalog No.	Species
NBL1-12381	Cytokeratin 14 Lysate
		0.1 mg / €370.00
		Novus Biologicals Inc.

Recommended Secondary Antibodies for Cytokeratin 14 (8 products)

Catalog No.	Host	Pres.
R1253AP	Mouse IgG (H+L chain) antibody
	Rabbit	AP
			Acris Antibodies GmbH
R1253B	Mouse IgG (H+L chain) antibody
	Rabbit	Biotin
			Acris Antibodies GmbH
R1253F	Mouse IgG (H+L chain) antibody
	Rabbit	FITC
			Acris Antibodies GmbH
R1253HRP	Mouse IgG (H+L chain) antibody
	Rabbit	HRP
			Acris Antibodies GmbH
R1253TR	Mouse IgG (H+L chain) antibody
	Rabbit	Texas Red
			Acris Antibodies GmbH
R1403R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Rabbit	PE
			Acris Antibodies GmbH
R1405R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Goat	PE
			Acris Antibodies GmbH
R1455R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Donkey	PE
			Acris Antibodies GmbH