BP5077 Cytokeratin 15 antibody

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0.1 ml / €340.00
Delivery Time: 2-3 working days

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Guinea Pig anti Human, Mouse Cytokeratin 15

BP5077

Product Description for Cytokeratin 15

Guinea Pig anti Human, Mouse Cytokeratin 15.
Presentation: Serum
Product is tested for Frozen Sections, Immunocytochemistry/Immunofluorescence, Paraffin Sections.

Properties for Cytokeratin 15

Product Category Antibodies
Quantity 0.1 ml
Synonyms CK-15, Cytokeratin-15, K15, KRT15, KRTB, Keratin, Keratin 15, Keratin-15, Review 009, Type I Cytoskeletal 15
Presentation Serum
Reactivity Hu, Ms
Applications C, ICC/IF, P
Clonality Polyclonal
Host Guinea Pig
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH

Datasheet Extract

Immunogen
Swiss Prot Num:
P19012
Immunogen:
Synthetic peptide of Human Keratin K15 (formerly also designated cytokeratin 15), coupled to KLH.
AA Sequence:
SNFHINVEESVDGQ-C
Genename:
3866
Antibody type Ab
Application Immunofluorescence Microscopy on Frozen Sections.
Immunohistochemistry on Paraffin Sections (after microwave treatment or Trypsin).
Working Dilution: 1/100-1/200 for Immunohistochemistry.
Incubation Time: 1h at RT.
Positive Control: Sweat glands of human skin (e.g. foot sole).
Background Keratin 15 is a type I keratin without a defined type II partner. Keratin 15 is expressed primarily in the basal keratinocytes of stratified tissues, including the fetal epidermis and fetal nail. Expression of keratin 15 is downregulated in some hyperproliferating situations, such as psoriasis and hypertrophic scars. Because keratinocytes in psoriasis and hypertrophic scars are activated, it is suggested that keratin 15 expression is not compatible with keratinocyte activation and the keratin 15 gene is downregulated to maintain the activated phenotype.
General Readings
  • Jih DM, Lyle S, Elenitsas R, Elder DE, Cotsarelis G. Cytokeratin 15 expression in trichoepitheliomas and a subset of basal cell carcinomas suggests they originate from hair follicle stem cells. J Cutan Pathol. 1999 Mar;26(3):113-8. PubMed PMID: 10235375.
  • Leube RE, Bader BL, Bosch FX, Zimbelmann R, Achtstaetter T, Franke WW. Molecular characterization and expression of the stratification-related cytokeratins 4 and 15. J Cell Biol. 1988 Apr;106(4):1249-61. PubMed PMID: 2452170.
Storage Store the antibody undiluted at 2-8°C.
Shelf life: one year from despatch.
Format
Preservatives:
0.09% Sodium Azide
State:
Liquid Serum
Serum
Specificity
Specificity:
MW 50,000 (pI 4.9) intermediate filament polypeptide, keratin K15, detected by Immunohistochemistry in basal cells of stratified epithelia (including epidermis, tongue, esophagus, exocervix) and complex stratified epithelia (e.g. bronchial epithelium), in luminal epithelia of mammary, salivary and sweat gland. In the hair follicle, staining was restricted to the basal cell layer of the outer root sheath.
Completely non-reactive were cells of liver, colon.
Species:
Human and Mouse.
Gene ID 3866
FocusOn and Reviews
Reviews:
Targets

Accessory Products

Proteins and/or Positive Controls

Positive controls for Cytokeratin 15 (2 products)

Catalog No. Species Pres. Purity   Source  
NBL1-12382

Cytokeratin 15 Lysate

Western Blot: Cytokeratin 15 Lysate [NBL1-12382] - Western Blot experiments.  Left-Control; Right -Over-expression Lysate for KRT15.
0.1 mg / €370.00
  Novus Biologicals Inc.
H00003866-T01

KRT15 293T Cell Transient Overexpression Lysate(Denatured)

KRT15 293T Cell Transient Overexpression Lysate(Denatured)
0.1 ml / €240.00
  Abnova Taiwan Corp.