Cytokeratin 18 (N-term) - AM08175PU-N

Product Description for Cytokeratin 18

Mouse anti Cytokeratin 18 SB38b.
Properties: (N-term)
Presentation: Purified
Product is tested for Frozen Sections, Enzyme Immunoassay, Western blot / Immunoblot.

Properties for Cytokeratin 18

Product Category	Antibodies
Quantity	0.1 mg
Synonyms	CK18, CYK18, Cell proliferation-inducing gene 46 protein, Cytokeratin-18, K18, KRT18, Keratin 18, Keratin type I cytoskeletal 18, Keratin-18, Review 009
Presentation	Purified
Applications	C, E, WB
Clonality	Monoclonal
Clone	SB38b
Host	Mouse
Isotype	IgG2b
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	Acris Antibodies GmbH
Material safety datasheet	MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen	Swiss Prot Num: P05783 Immunogen: Recombinant N-terminal fragment of keratin 18. Genename: 3875
Property	N-term
Antibody type	Ab
Add. information	Chatacterization: To ensure acceptable performance, each batch of product is tested by ELISA, Immunohistochemical staining (HT29 and HCC38 cells) and Western blotting (HT29 and HCC38 cell lysates) to conform to characteristics of a standard reference reagent.
Application	ELISA: 1/5,000-1/20,000. Western Blot: 1/2,000-1/4,000. Immunohistochemistry (Acetone-Fixed, Frozen Tissue Sections): 10 µg/ml
Background	Keratins are a family of intermediate filament proteins that assemble into filaments through forming heterodimers of one type I keratin (keratins 9 to 23) and one type II keratin (keratins 1-8). The two keratin types share only 30% sequence homology. (Ref.1-6) Keratins demonstrate tissue- and differentiation-specific expression profiles. Cytokeratin 18, also known as keratin 18, is a type I intermediate filament protein of approximately 48 kDa. It exists as a heterotetramer composed of two type I and two type II keratins. Cytokeratin 18 is expressed in all simple type epithelia and basal cells of many squamous and non-epidermal epithelia, and is often co-expressed with cytokeratin 8. (Ref.6-8) Tissues from the gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.
Concentration	0.1 mg/ml
General Readings	Lodish, H. et al. (2000) In: Molecular Cell Biology. NY, NY, W.H. Freeman and Co., pp 795-847. Fuchs, E.V. et al. (1981) Cell 27: 75-84. Hanukoglu, I. And E. Fuchs. (1983) Cell 33: 915-924. Schiller, D.L. et al. (1982) EMBO J. 6:761-769. Tseng, S.C.G. et al. (1982) Cell 30: 361-372. Ku, N. et al. (2003) Proc. Natl Acad. Sci. USA 100: 6063. Kulesh, D.A. and R.G. Oshima (1989) Genomics 4: 339. Steinart, P.M. and D.R. Roop (1988) Ann. Rev. Bioche,m. 57: 593.
Storage	Store the antibody undiluted at 2-8°C. Shelf life: one year from despatch.
Format	Buffer System: 100 mM Borate buffered saline, pH 8.0 without preservatives or amine-containing buffer salts. State: Liquid purified Ig fraction. Purified
Specificity	Specificity: This antibody reacts specifically specifically with Keratin 18 and recombinant N-terminal Keratin 18.
Gene ID	3875
FocusOn and Reviews	Reviews: Antibodies to Cytokeratin Proteins - Product Review 09
Targets	Cytokeratin 18 antibody

Accessory Products

Proteins and/or Positive Controls

Proteins for Cytokeratin 18 (7 products)

Catalog No.	Species	Pres.	Purity	Source
AR09362PU-N	Cytokeratin 18
	Human	Purified	> 95 %		0.25 mg / €530.00
					Acris Antibodies GmbH
AR09362PU-S	Cytokeratin 18
	Human	Purified	> 95 %		0.1 mg / €320.00
					Acris Antibodies GmbH
H00003875-P01-10	Cytokeratin 18
	Human			in vitro transl.	10 µg / €350.00
					Abnova Taiwan Corp.
H00003875-P01-25	Cytokeratin 18
	Human			in vitro transl.	25 µg / €500.00
					Abnova Taiwan Corp.
H00003875-P02	Cytokeratin 18
	Human	Purified			10 µg / €420.00
					Abnova Taiwan Corp.
H00003875-P02-10	Cytokeratin 18
	Human			in vitro transl.	10 µg / €350.00
					Abnova Taiwan Corp.
H00003875-P02-25	Cytokeratin 18
	Human			in vitro transl.	25 µg / €500.00
					Abnova Taiwan Corp.

Positive controls for Cytokeratin 18 (3 products)

Catalog No.	Species
NBL1-12385	Cytokeratin 18 Lysate
		0.1 mg / €370.00
		Novus Biologicals Inc.
NBL1-12386	Cytokeratin 18 Lysate
		0.1 mg / €370.00
		Novus Biologicals Inc.
H00003875-T01	KRT18 293T Cell Transient Overexpression Lysate(Denatured)
		0.1 ml / €240.00
		Abnova Taiwan Corp.

Recommended Secondary Antibodies for Cytokeratin 18 (8 products)

Catalog No.	Host	Pres.
R1253AP	Mouse IgG (H+L chain) antibody
	Rabbit	AP
			Acris Antibodies GmbH
R1253B	Mouse IgG (H+L chain) antibody
	Rabbit	Biotin
			Acris Antibodies GmbH
R1253F	Mouse IgG (H+L chain) antibody
	Rabbit	FITC
			Acris Antibodies GmbH
R1253HRP	Mouse IgG (H+L chain) antibody
	Rabbit	HRP
			Acris Antibodies GmbH
R1253TR	Mouse IgG (H+L chain) antibody
	Rabbit	Texas Red
			Acris Antibodies GmbH
R1403R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Rabbit	PE
			Acris Antibodies GmbH
R1405R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Goat	PE
			Acris Antibodies GmbH
R1455R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Donkey	PE
			Acris Antibodies GmbH