Switch to Acris Antibodies Inc. (North America) Switch to Acris Antibodies GmbH (Europe/Worldwide)
Home » Antibodies » Cytokeratin 18

BM2275B Cytokeratin 18 antibody

See related secondary antibodies

Search for all "Cytokeratin 18"

0.25 ml / €330.00
Please visit the appropriate website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti Bovine, Canine, Hamster, Human, Mouse, Porcine, Rat, Sheep, Trout Cytokeratin 18 Ks18.04

BM2275B

Product Description for Cytokeratin 18

Mouse anti Bovine, Canine, Hamster, Human, Mouse, Porcine, Rat, Sheep, Trout Cytokeratin 18 Ks18.04.
Presentation: Biotin
Product is tested for Frozen Sections, Enzyme Immunoassay, Western blot / Immunoblot, Paraffin Sections.

Properties for Cytokeratin 18

Product Category Antibodies
Quantity 0.25 ml
Synonyms CK18, CYK18, Cell proliferation-inducing gene 46 protein, Cytokeratin-18, K18, KRT18, Keratin 18, Keratin type I cytoskeletal 18, Keratin-18, Review 009
Presentation Biotin
Reactivity Bov, Can, Hst, Hu, Ms, Por, Rt, Sh, Trout
Applications C, E, P, WB
Clonality Monoclonal
Clone Ks18.04
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH

Datasheet Extract

Immunogen
Swiss Prot Num:
P05783
Immunogen:
Cytokeratin 18 of molecular weight 45kDa. Purified from a HeLa cytoskeletal preparation.
Genename:
3875
Antibody type Ab
Application ELISA.
Immunoblotting. 
Immunohistochemistry on Frozen Sections
Immunohistochemistry on Paraffin Sections. 
Recommended Dilution: 1/10 for Immunohistochemistry. 
Incubation Time: 1h at 37°C.
Background Cytokeratin 18 is an acidic keratin which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas. Cytokeratin 18 exists in combination with Cytokeratin 8, a basic keratin. Hepatocellular carcinomas have been reportedly defined by the use of antibodies that recognize only Cytokeratins 8 and 18.
Product Citations Unconjugated antibody is cited in:
1. Hantel C, Lewrick F, Schneider S, Zwermann O, Perren A, Reincke M, et al. Anti insulin-like growth factor I receptor immunoliposomes: a single formulation combining two anticancer treatments with enhanced therapeutic efficiency. J Clin Endocrinol Metab. 2010 Feb;95(2):943-52. doi: 10.1210/jc.2009-1980. Epub 2009 Dec 1. PubMed PMID: 19952224.
General Readings
  • Bártek J, Vojtĕsek B, Stasková Z, Bártková J, Kerekés Z, Rejthar A, et al. A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology. J Pathol. 1991 Jul;164(3):215-24. PubMed PMID: 1716305.
  • Lauerová L, Kovarik J, Bártek J, Rejthar A, Vojtĕsek B. Novel monoclonal antibodies defining epitope of human cytokeratin 18 molecule. Hybridoma. 1988 Oct;7(5):495-504. PubMed PMID: 2461901.
  • Markl J, Winter S, Franke WW: The catalog and the expression complexity of cytokeratins in a lower vertebrate: Biochemical identification of cytokerations in a teleost fish, the rainbow trout. Eur. J. Cell Biol. 50, 1-16 (1989)
  • Moll R, Franke WW, Schiller DL, Geiger B, Krepler R. The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells. Cell. 1982 Nov;31(1):11-24. PubMed PMID: 6186379.
  • Gomi H, Yokoyama T, Fujimoto K, Ikeda T, Katoh A, Itoh T, et al. Mice devoid of the glial fibrillary acidic protein develop normally and are susceptible to scrapie prions. Neuron. 1995 Jan;14(1):29-41. PubMed PMID: 7826639.
  • Bantel H, Ruck P, Gregor M, Schulze-Osthoff K. Detection of elevated caspase activation and early apoptosis in liver diseases. Eur J Cell Biol. 2001 Mar;80(3):230-9. PubMed PMID: 11322387.
  • Stumptner C, Fuchsbichler A, Heid H, Zatloukal K, Denk H: Mallory body - a disease-associated type of sequestosome. Hepatology 35, 1053-1062 (2002)
  • Ikenouchi J, Matsuda M, Furuse M, Tsukita S. Regulation of tight junctions during the epithelium-mesenchyme transition: direct repression of the gene expression of claudins/occludin by Snail. J Cell Sci. 2003 May 15;116(Pt 10):1959-67. Epub 2003 Mar 26. PubMed PMID: 12668723.
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Label:
Biotin
Purification:
Affinity Chromatography on Protein A
Preservatives:
0.09% Sodium Azide
State:
Liquid purified Ig fraction
Aff - Purified
Specificity
Specificity:
This Monoclonal antibody Ks 18.04 represents an excellent marker to discriminate simple epithelia from those of different origin.
Tumors Specifically Detected: all adenocarcinoma; mammary carcinoma, urinary bladder carcinoma, undifferentiated carcinoma, cervix carcinoma, hepatocellular carcinoma.
Polypeptide Reacting: Mr 45 000 polypeptide (Human keratin K18; formerly also designated cytokeratin 18) of all simple type epithelia and basal cells of many squamous, nonepidermal epithelia. 
Tested Reactivities on Cultured Cell Lines:  HeLa and MCF-7.
Species:
Human, Mouse, Rat, Bovine, Pig, Dog, Hamster,Sheep, Fish (trout).
Gene ID 3875
FocusOn and Reviews
Reviews:
Targets

Accessory Products

Proteins and/or Positive Controls

Proteins for Cytokeratin 18 (7 products)

Catalog No. Species Pres. Purity   Source  
AR09362PU-N

Cytokeratin 18
Cytokeratin 18 Human Purified > 95 %
0.25 mg / €530.00
  Acris Antibodies GmbH
AR09362PU-S

Cytokeratin 18
Cytokeratin 18 Human Purified > 95 %
0.1 mg / €320.00
  Acris Antibodies GmbH
H00003875-P01-10

Cytokeratin 18
Cytokeratin 18 Human in vitro transl.
10 µg / €350.00
  Abnova Taiwan Corp.
H00003875-P01-25

Cytokeratin 18
Cytokeratin 18 Human in vitro transl.
25 µg / €500.00
  Abnova Taiwan Corp.
H00003875-P02

Cytokeratin 18
Cytokeratin 18 Human Purified
10 µg / €420.00
  Abnova Taiwan Corp.
H00003875-P02-10

Cytokeratin 18
Cytokeratin 18 Human in vitro transl.
10 µg / €350.00
  Abnova Taiwan Corp.
H00003875-P02-25

Cytokeratin 18
Cytokeratin 18 Human in vitro transl.
25 µg / €500.00
  Abnova Taiwan Corp.

Positive controls for Cytokeratin 18 (3 products)

Catalog No. Species Pres. Purity   Source  
NBL1-12385

Cytokeratin 18 Lysate
Western Blot: Cytokeratin 18 Lysate [NBL1-12385] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for KRT18
0.1 mg / €370.00
  Novus Biologicals Inc.
NBL1-12386

Cytokeratin 18 Lysate
Western Blot: Cytokeratin 18 Lysate [NBL1-12386] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for KRT18.
0.1 mg / €370.00
  Novus Biologicals Inc.
H00003875-T01

KRT18 293T Cell Transient Overexpression Lysate(Denatured)
KRT18 293T Cell Transient Overexpression Lysate(Denatured)
0.1 ml / €240.00
  Abnova Taiwan Corp.

Recommended Secondary Antibodies for Cytokeratin 18 (8 products)

Catalog No. Host Clone/Iso. Pres.   React. Applications  
R1253AP

Mouse IgG (H+L chain) antibody
Mouse IgG (H+L chain) antibody Rabbit AP
    Acris Antibodies GmbH
R1253B

Mouse IgG (H+L chain) antibody
Mouse IgG (H+L chain) antibody Rabbit Biotin
    Acris Antibodies GmbH
R1253F

Mouse IgG (H+L chain) antibody
Mouse IgG (H+L chain) antibody Rabbit FITC
    Acris Antibodies GmbH
R1253HRP

Mouse IgG (H+L chain) antibody
Mouse IgG (H+L chain) antibody Rabbit HRP
    Acris Antibodies GmbH
R1253TR

Mouse IgG (H+L chain) antibody
Mouse IgG (H+L chain) antibody Rabbit Texas Red
    Acris Antibodies GmbH
R1403R

Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody Rabbit PE
    Acris Antibodies GmbH
R1405R

Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody Goat PE
    Acris Antibodies GmbH
R1455R

Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody Donkey PE
    Acris Antibodies GmbH
Click here to see all secondary antibodies for Cytokeratin 18
Compare Products

You have no items to compare.

My Cart

You have no items in your shopping cart.

DIN-EN-ISO-9001
Acris Antibodies Newsletter
PTG money back guarantee
Link-A-Light Conjugation Kits
Protein Biotechnologies - Ready to use lysates, cell lines, tissue microarrays and more...
Antibody Location Service

Antibody Location Service

Over 230,000 products but you can't find the right antibody for your protein or application?
Acris will do the search for you!

Free Antibody Location Service
Service
 
© Copyright 1998 - 2013 Acris Antibodies GmbH