Product Description for Cytokeratin 18
Mouse anti Bovine, Canine, Hamster, Human, Mouse, Porcine, Rat, Sheep, Trout, Zebrafish Cytokeratin 18 Ks18.04.
Presentation: Purified
Product is tested for Frozen Sections, Western blot / Immunoblot, Paraffin Sections.
Presentation: Purified
Product is tested for Frozen Sections, Western blot / Immunoblot, Paraffin Sections.
Properties for Cytokeratin 18
| Product Category | Antibodies |
|---|---|
| Quantity | 50 µg |
| Synonyms | CK18, CYK18, Cell proliferation-inducing gene 46 protein, Cytokeratin-18, K18, KRT18, Keratin 18, Keratin type I cytoskeletal 18, Keratin-18, Review 009 |
| Presentation | Purified |
| Reactivity | Bov, Can, Hst, Hu, Ms, Por, Rt, Sh, Trout, Ze |
| Applications | C, P, WB |
| Clonality | Monoclonal |
| Clone | Ks18.04 |
| Host | Mouse |
| Isotype | IgG1 |
| Shipping to | Worldwide |
| PDF datasheet | View Datasheet |
| Manufacturer | Acris Antibodies GmbH |
Datasheet Extract
| Immunogen |
|
|---|---|
| Antibody type | Ab |
| Application | Western blot. Immunohistochemistry on Frozen and Paraffin sections: 1/20 with PBS Incubation time: 1h at 37°C, extended with paraffin sections (overnight at 2-8°C). |
| Background | Cytokeratin 18 is an acidic keratin which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas. Cytokeratin 18 exists in combination with Cytokeratin 8, a basic keratin. Hepatocellular carcinomas have been reportedly defined by the use of antibodies that recognize only Cytokeratins 8 and 18. |
| Product Citations |
|
| General Readings | Bartek, J. et al. J. Pathol. 164, 214-224 (1991) Lanerova, L. et al. Hybridoma 7, 495-504 (1988) Markl, J. et al. Eur. J. Cell Biol. 50, 1-16 (1989) Moll, R., et al. Cell 31, 11-24 (1982) Gomi, H., et al. Neuron, Vol. 14, 29-41 (1995) Bantel, H. et al. Eur. J. Cell Biol. 80, 230-239 (2001) Stumpter, C et al. Hepatology 35, 1035-1062 (2002) Ikenouchi, J et al. J Cell Sci 116, 1959-1967 (2003) Schaffeld, M et al. Differentiation 71, 73-82 (2003) |
| Storage | Store the original vial at 2-8°C. Following reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| Format |
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| Species Reactivity |
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| Specificity |
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| Gene ID | 3875 |
| FocusOn and Reviews | |
| Targets |
Accessory Products
Proteins and/or Positive Controls
Proteins for Cytokeratin 18 (7 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| AR09362PU-N |
Cytokeratin 18 |
||||||
 |
Human | Purified | > 95 % |
0.25 mg /
€530.00
|
|||
| Acris Antibodies GmbH | |||||||
| AR09362PU-S |
Cytokeratin 18 |
||||||
|
Human | Purified | > 95 % |
0.1 mg /
€320.00
|
|||
| Acris Antibodies GmbH | |||||||
| H00003875-P01-10 |
Cytokeratin 18 |
||||||
|
Human | in vitro transl. |
10 µg /
€350.00
|
||||
| Abnova Taiwan Corp. | |||||||
| H00003875-P01-25 |
Cytokeratin 18 |
||||||
|
Human | in vitro transl. |
25 µg /
€500.00
|
||||
| Abnova Taiwan Corp. | |||||||
| H00003875-P02 |
Cytokeratin 18 |
||||||
|
Human | Purified |
10 µg /
€420.00
|
||||
| Abnova Taiwan Corp. | |||||||
| H00003875-P02-10 |
Cytokeratin 18 |
||||||
|
Human | in vitro transl. |
10 µg /
€350.00
|
||||
| Abnova Taiwan Corp. | |||||||
| H00003875-P02-25 |
Cytokeratin 18 |
||||||
|
Human | in vitro transl. |
25 µg /
€500.00
|
||||
| Abnova Taiwan Corp. | |||||||
Positive controls for Cytokeratin 18 (3 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| NBL1-12385 |
Cytokeratin 18 Lysate |
||||||
![Western Blot: Cytokeratin 18 Lysate [NBL1-12385] - Western Blot experiments.
Left-Control; Right -Over-expression Lysate for KRT18](http://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/Novus/Cytokeratin 18 Lysate-Western Blot-NBL1-12385-img0001.jpg) |
0.1 mg /
€370.00
|
||||||
| Novus Biologicals Inc. | |||||||
| NBL1-12386 |
Cytokeratin 18 Lysate |
||||||
![Western Blot: Cytokeratin 18 Lysate [NBL1-12386] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for KRT18.](http://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/Novus/Cytokeratin 18 Lysate-Western Blot-NBL1-12386-img0001.jpg) |
0.1 mg /
€370.00
|
||||||
| Novus Biologicals Inc. | |||||||
| H00003875-T01 |
KRT18 293T Cell Transient Overexpression Lysate(Denatured) |
||||||
|
0.1 ml /
€240.00
|
||||||
| Abnova Taiwan Corp. | |||||||
Recommended Secondary Antibodies for Cytokeratin 18 (8 products)
| Catalog No. | Host | Clone/Iso. | Pres. | React. | Applications | ||
|---|---|---|---|---|---|---|---|
| R1253AP |
Mouse IgG (H+L chain) antibody |
||||||
|
|
Rabbit | AP | |||||
| Acris Antibodies GmbH | |||||||
| R1253B |
Mouse IgG (H+L chain) antibody |
||||||
|
|
Rabbit | Biotin | |||||
| Acris Antibodies GmbH | |||||||
| R1253F |
Mouse IgG (H+L chain) antibody |
||||||
|
|
Rabbit | FITC | |||||
| Acris Antibodies GmbH | |||||||
| R1253HRP |
Mouse IgG (H+L chain) antibody |
||||||
|
Rabbit | HRP | |||||
| Acris Antibodies GmbH | |||||||
| R1253TR |
Mouse IgG (H+L chain) antibody |
||||||
|
|
Rabbit | Texas Red | |||||
| Acris Antibodies GmbH | |||||||
| R1403R |
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
||||||
|
|
Rabbit | PE | |||||
| Acris Antibodies GmbH | |||||||
| R1405R |
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
||||||
|
|
Goat | PE | |||||
| Acris Antibodies GmbH | |||||||
| R1455R |
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
||||||
|
|
Donkey | PE | |||||
| Acris Antibodies GmbH | |||||||
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