Cytokeratin 18

Product Description for Cytokeratin 18

Mouse anti Bovine, Human Cytokeratin 18 Ks18.27.
Presentation: Purified
Product is tested for Frozen Sections, Enzyme Immunoassay, Western blot / Immunoblot.

Properties for Cytokeratin 18

Product Category	Antibodies
Quantity	50 µg
Synonyms	CK18, CYK18, Cell proliferation-inducing gene 46 protein, Cytokeratin-18, K18, KRT18, Keratin 18, Keratin type I cytoskeletal 18, Keratin-18, Review 009
Presentation	Purified
Reactivity	Bov, Hu
Applications	C, E, WB
Clonality	Monoclonal
Clone	Ks18.27
Host	Mouse
Isotype	IgG1
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	Acris Antibodies GmbH
Material safety datasheet	MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen	Swiss Prot Num: P05783 Immunogen: Cytoskeletal preparation from tumor cell line MCF-7. Genename: 3875
Antibody type	Ab
Application	Immunohistochemistry on frozen sections: 1:10; 1 h at RT. Cytological material. Immunoblotting. ELISA.
Background	Cytokeratin 18 is an acidic keratin which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas. Cytokeratin 18 exists in combination with Cytokeratin 8, a basic keratin. Hepatocellular carcinomas have been reportedly defined by the use of antibodies that recognize only Cytokeratins 8 and 18.
General Readings	Moll R, Achtstätter T, Becht E, Balcarova-Ständer J, Ittensohn M, Franke WW. Cytokeratins in normal and malignant transitional epithelium. Maintenance of expression of urothelial differentiation features in transitional cell carcinomas and bladder carcinoma cell culture lines. Am J Pathol. 1988 Jul;132(1):123-44. PubMed PMID: 2456018. Moll R, Franke WW, Schiller DL, Geiger B, Krepler R. The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells. Cell. 1982 Nov;31(1):11-24. PubMed PMID: 6186379. Moll R, Schiller DL, Franke WW. Identification of protein IT of the intestinal cytoskeleton as a novel type I cytokeratin with unusual properties and expression patterns. J Cell Biol. 1990 Aug;111(2):567-80. PubMed PMID: 1696264.
Storage	Prior to reconstitution store at 2-8°C. Following reconstitution store the antibody at -20°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Format	Purification: Protein A affinity chromatography Buffer System: PBS buffer, pH 7.4 containing 0.09 % sodium azide, 0.5% BSA State: Lyophilized purified IgG Purified Reconstitution: Restore in 1 ml dist. water
Specificity	Specificity: Ks 18.27 represents an excellent marker to discriminate simple epithelia from those of different origin. Polypeptide reacting: Mr 45,000 polypeptide (keratin K18; formerly also designated cytokeratin 18) of all simple type and complex epithelia. Reacts with human, bovine; negative with mouse. Reacts with MCF-7, HeLa. A-431, PLC, RT 112. All adeno-CA tested; undifferentiated CA; cervix CA; hepatocellular CA. Species: Human, Bovine.
Gene ID	3875
FocusOn and Reviews	Reviews: Antibodies to Cytokeratin Proteins - Product Review 09
Targets	Cytokeratin 18 antibody

Accessory Products

Proteins and/or Positive Controls

Proteins for Cytokeratin 18 (7 products)

Catalog No.	Species	Pres.	Purity	Source
AR09362PU-N	Cytokeratin 18
	Human	Purified	> 95 %		0.25 mg / €530.00
					Acris Antibodies GmbH
AR09362PU-S	Cytokeratin 18
	Human	Purified	> 95 %		0.1 mg / €320.00
					Acris Antibodies GmbH
H00003875-P01-10	Cytokeratin 18
	Human			in vitro transl.	10 µg / €350.00
					Abnova Taiwan Corp.
H00003875-P01-25	Cytokeratin 18
	Human			in vitro transl.	25 µg / €500.00
					Abnova Taiwan Corp.
H00003875-P02	Cytokeratin 18
	Human	Purified			10 µg / €420.00
					Abnova Taiwan Corp.
H00003875-P02-10	Cytokeratin 18
	Human			in vitro transl.	10 µg / €350.00
					Abnova Taiwan Corp.
H00003875-P02-25	Cytokeratin 18
	Human			in vitro transl.	25 µg / €500.00
					Abnova Taiwan Corp.

Positive controls for Cytokeratin 18 (3 products)

Catalog No.	Species
NBL1-12385	Cytokeratin 18 Lysate
		0.1 mg / €370.00
		Novus Biologicals Inc.
NBL1-12386	Cytokeratin 18 Lysate
		0.1 mg / €370.00
		Novus Biologicals Inc.
H00003875-T01	KRT18 293T Cell Transient Overexpression Lysate(Denatured)
		0.1 ml / €240.00
		Abnova Taiwan Corp.

Recommended Secondary Antibodies for Cytokeratin 18 (8 products)

Catalog No.	Host	Pres.
R1253AP	Mouse IgG (H+L chain) antibody
	Rabbit	AP
			Acris Antibodies GmbH
R1253B	Mouse IgG (H+L chain) antibody
	Rabbit	Biotin
			Acris Antibodies GmbH
R1253F	Mouse IgG (H+L chain) antibody
	Rabbit	FITC
			Acris Antibodies GmbH
R1253HRP	Mouse IgG (H+L chain) antibody
	Rabbit	HRP
			Acris Antibodies GmbH
R1253TR	Mouse IgG (H+L chain) antibody
	Rabbit	Texas Red
			Acris Antibodies GmbH
R1403R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Rabbit	PE
			Acris Antibodies GmbH
R1405R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Goat	PE
			Acris Antibodies GmbH
R1455R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Donkey	PE
			Acris Antibodies GmbH