BM6006P Cytokeratin 18 antibody

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0.1 mg / €300.00
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Mouse anti Human Cytokeratin 18 RCK106

BM6006P

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  • BM6006P

Product Description for Cytokeratin 18

Mouse anti Human Cytokeratin 18 RCK106.
Presentation: Purified
Product is tested for Paraffin Sections, Frozen Sections, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot, Flow Cytometry.

Properties for Cytokeratin 18

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms CK18, CYK18, Cell proliferation-inducing gene 46 protein, Cytokeratin-18, K18, KRT18, Keratin 18, Keratin type I cytoskeletal 18, Keratin-18
Presentation Purified
Reactivity Hu
Applications C, F, ICC/IF, P, WB
Clonality Monoclonal
Clone RCK106
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P05783
Immunogen:
Cytokeratins from the Human bladder carcinoma cell line T24.
Genename:
3875
Antibody type Ab
Isotype control AM03095PU-N, SM10P (for use in human samples)
Application Immunocytochemistry. 
Immunohistochemistry on Frozen Sections.
Immunohistochemistry on Paraffin Sections. 

Immunoblotting.
Flow Cytometry. 
Recommended Dilutions: 1/100–1/200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1/100–1/1000 for immunoblotting applications.
Background Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9-7.8. The individual human cytokeratins are numbered 1 to 20.
The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium.
Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
Concentration 1.0 mg/ml
General Readings
  1. Ramaekers F, Huysmans A, Schaart G, Moesker O, Vooijs P. Tissue distribution of keratin 7 as monitored by a monoclonal antibody. Exp Cell Res. 1987 May;170(1):235-49. PubMed PMID: 2436934.
  2. Raats JM, Pieper FR, Vree Egberts WT, Verrijp KN, Ramaekers FC, Bloemendal H. Assembly of amino-terminally deleted desmin in vimentin-free cells. J Cell Biol. 1990 Nov;111(5 Pt 1):1971-85. PubMed PMID: 1699950. (Free PMC Article available)
  3. Smedts F, Ramaekers F, Robben H, Pruszczynski M, van Muijen G, Lane B, et al. Changing patterns of keratin expression during progression of cervical intraepithelial neoplasia. Am J Pathol. 1990 Mar;136(3):657-68. PubMed PMID: 1690513. (Free PMC Article available)
  4. Ramaekers F, van Niekerk C, Poels L, Schaafsma E, Huijsmans A, Robben H, et al. Use of monoclonal antibodies to keratin 7 in the differential diagnosis of adenocarcinomas. Am J Pathol. 1990 Mar;136(3):641-55. PubMed PMID: 1690512. (Free PMC Article available)
  5. Schaafsma HE, Ramaekers FC, van Muijen GN, Lane EB, Leigh IM, Robben H, et al. Distribution of cytokeratin polypeptides in human transitional cell carcinomas, with special emphasis on changing expression patterns during tumor progression. Am J Pathol. 1990 Feb;136(2):329-43. PubMed PMID: 1689541. (Free PMC Article available)
  6. Ivanyi D, Groeneveld E, Van Doornewaard G, Mooi WJ, Hageman PC. Keratin subtypes in carcinomas of the uterine cervix: implications for histogenesis and differential diagnosis. Cancer Res. 1990 Aug 15;50(16):5143-52. PubMed PMID: 1696167.
  7. Smedts, F., Ramaekers, F., Troyanovsky, S., Pruszczynski, M., Link, M., Lane, B., Leigh, I., Schijf, C., and Vooijs, P. (1992). Keratin expression in cervical cancer, Am J Pathol 141, 497-511.
  8. Bauwens LJ, De Groot JC, Ramaekers FC, Veldman JE, Huizing EH. Expression of intermediate filament proteins in the adult human vestibular labyrinth. Ann Otol Rhinol Laryngol. 1992 Jun;101(6):479-86. PubMed PMID: 1376975.
  9. Bonfrer JM, Groeneveld EM, Korse CM, van Dalen A, Oomen LC, Ivanyi D. Monoclonal antibody M3 used in tissue polypeptide-specific antigen assay for the quantification of tissue polypeptide antigen recognizes keratin 18. Tumour Biol. 1994;15(4):210-22. PubMed PMID: 7524130.
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freeze-thaw cycles.
Shelf life: One year from despatch.
Format
Buffer System:
PBS
Preservatives:
0.09% Sodium Azide
State:
Liquid purified IgG fraction
Purified
Species Reactivity
Species reactivity (tested):
Human.
Specificity
Specificity:
RCK106 reacts exclusively with Cytokeratin 18 in glandular epithelial cells of the digestive, respiratory, and urogenital tracts, endocrine and exocrine cells and mesothelial cells, as well as adenocarcinomas originating from them.
Reacts with Human exclusively.
Gene ID 3875
FocusOn and Reviews
Reviews:

Accessory Products

Proteins and/or Positive Controls

Proteins for Cytokeratin 18 (7 products)

Catalog No. Species Pres. Purity   Source  
AR09362PU-N

Cytokeratin 18

Cytokeratin 18 Human Purified > 95 % (determined by SDS gelelectrophoresis)
0.25 mg / €530.00
  Acris Antibodies GmbH
AR09362PU-S

Cytokeratin 18

Cytokeratin 18 Human Purified > 95 % (determined by SDS gelelectrophoresis)
0.1 mg / €320.00
  Acris Antibodies GmbH
H00003875-P01-10

Cytokeratin 18

Cytokeratin 18 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €350.00
  Abnova Taiwan Corp.
H00003875-P01-25

Cytokeratin 18

Cytokeratin 18 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €500.00
  Abnova Taiwan Corp.
H00003875-P02

Cytokeratin 18

Cytokeratin 18 Human Purified
10 µg / €420.00
  Abnova Taiwan Corp.
H00003875-P02-10

Cytokeratin 18

Cytokeratin 18 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €350.00
  Abnova Taiwan Corp.
H00003875-P02-25

Cytokeratin 18

Cytokeratin 18 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €500.00
  Abnova Taiwan Corp.

Positive controls for Cytokeratin 18 (3 products)

Catalog No. Species Pres. Purity   Source  
NBL1-12385

Cytokeratin 18 Lysate

Western Blot: Cytokeratin 18 Lysate [NBL1-12385] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for KRT18
0.1 mg / €370.00
  Novus Biologicals Inc.
NBL1-12386

Cytokeratin 18 Lysate

Western Blot: Cytokeratin 18 Lysate [NBL1-12386] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for KRT18.
0.1 mg / €370.00
  Novus Biologicals Inc.
H00003875-T01

KRT18 293T Cell Transient Overexpression Lysate(Denatured)

KRT18 293T Cell Transient Overexpression Lysate(Denatured)
0.1 ml / €240.00
  Abnova Taiwan Corp.
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