Cytokeratin 18

Product Description for Cytokeratin 18

Mouse anti Human, Rat Cytokeratin 18 CK-18.
Product is tested for Frozen Sections, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western blot / Immunoblot.

Properties for Cytokeratin 18

Product Category	Antibodies
Quantity	0.1 mg
Synonyms	CK18, CYK18, Cell proliferation-inducing gene 46 protein, Cytokeratin-18, K18, KRT18, Keratin 18, Keratin type I cytoskeletal 18, Keratin-18, Review 009
Reactivity	Hu, Rt
Applications	C, ICC/IF, IP, WB
Clonality	Monoclonal
Clone	CK-18
Host	Mouse
Isotype	IgG1
Shipping to	Not USA/Canada
PDF datasheet	View Datasheet
Manufacturer	Novus Biologicals Inc.

Datasheet Extract

Immunogen	Swiss Prot Num: P05783 Immunogen: The human epidermal carcinoma A-431 and MCF-7 human breast cancer cell lines., Genename: 3875
Antibody type	Ab
Add. information	,
Application	This antibody is useful in Western Blot, Immunohistochemistry, Immunoprecipitation, Immunofluorescence., Western Blot 0.5-1ul/ml, Immunohistochemistry-Paraffin 1-2ul/ml, Immunohistochemistry-Frozen 0.5-2ul/ml.
Background	Cytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinizing tissue (ie., skin) and non keratinizing cells (ie. mesothelial cells). Although not a traditional marker for endothelial cells, cytokeratins have also been found in some microvascular endothelial cells. At least 20 different cytokeratins (CK) in the molecular range of 40-70 kDa and isoelectric points of 5-8.5 can be identified using two dimensional gel electrophoresis. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells. Monoclonal antibodies to cytokeratin proteins can be useful markers for tumor identification and classification. Cytokeratin 18 is an acidic keratin which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas. Cytokeratin 18 exists in combination with Cytokeratin 8, a basic keratin. Hepatocellular carcinomas have been reportedly defined by the use of antibodies that recognize only Cytokeratins 8 and 18. This antibody reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid.
General Readings	,
Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Format	Purification: Affinity purified Buffer System: PBS
Specificity	Species: Human, Rat
Gene ID	3875
FocusOn and Reviews	Reviews: Antibodies to Cytokeratin Proteins - Product Review 09
Targets	Cytokeratin 18 antibody

Accessory Products

Proteins and/or Positive Controls

Proteins for Cytokeratin 18 (7 products)

Catalog No.	Species	Pres.	Purity	Source
AR09362PU-N	Cytokeratin 18
	Human	Purified	> 95 %		0.25 mg / €530.00
					Acris Antibodies GmbH
AR09362PU-S	Cytokeratin 18
	Human	Purified	> 95 %		0.1 mg / €320.00
					Acris Antibodies GmbH
H00003875-P01-10	Cytokeratin 18
	Human			in vitro transl.	10 µg / €350.00
					Abnova Taiwan Corp.
H00003875-P01-25	Cytokeratin 18
	Human			in vitro transl.	25 µg / €500.00
					Abnova Taiwan Corp.
H00003875-P02	Cytokeratin 18
	Human	Purified			10 µg / €420.00
					Abnova Taiwan Corp.
H00003875-P02-10	Cytokeratin 18
	Human			in vitro transl.	10 µg / €350.00
					Abnova Taiwan Corp.
H00003875-P02-25	Cytokeratin 18
	Human			in vitro transl.	25 µg / €500.00
					Abnova Taiwan Corp.

Positive controls for Cytokeratin 18 (3 products)

Catalog No.	Species
NBL1-12385	Cytokeratin 18 Lysate
		0.1 mg / €370.00
		Novus Biologicals Inc.
NBL1-12386	Cytokeratin 18 Lysate
		0.1 mg / €370.00
		Novus Biologicals Inc.
H00003875-T01	KRT18 293T Cell Transient Overexpression Lysate(Denatured)
		0.1 ml / €240.00
		Abnova Taiwan Corp.

Recommended Secondary Antibodies for Cytokeratin 18 (8 products)

Catalog No.	Host	Pres.
R1253AP	Mouse IgG (H+L chain) antibody
	Rabbit	AP
			Acris Antibodies GmbH
R1253B	Mouse IgG (H+L chain) antibody
	Rabbit	Biotin
			Acris Antibodies GmbH
R1253F	Mouse IgG (H+L chain) antibody
	Rabbit	FITC
			Acris Antibodies GmbH
R1253HRP	Mouse IgG (H+L chain) antibody
	Rabbit	HRP
			Acris Antibodies GmbH
R1253TR	Mouse IgG (H+L chain) antibody
	Rabbit	Texas Red
			Acris Antibodies GmbH
R1403R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Rabbit	PE
			Acris Antibodies GmbH
R1405R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Goat	PE
			Acris Antibodies GmbH
R1455R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Donkey	PE
			Acris Antibodies GmbH