Cytokeratin 5 (+ KRT8) - BM5042B | acris-antibodies.com

Product Description for Cytokeratin 5

Mouse anti Amphibian, Bovine, Human, Hydra, Mouse, Porcine Cytokeratin 5 C22.
Properties: (+ KRT8)
Presentation: Biotin
Product is tested for Frozen Sections, Enzyme Immunoassay, Immunocytochemistry/Immunofluorescence, Flow Cytometry, Paraffin Sections.

Properties for Cytokeratin 5

Product Category	Antibodies
Quantity	0.25 ml
Synonyms	58 kDa Cytokeratin, CK5, Cytokeratin-5, K5, KRT5, Keratin 5, Keratin type II cytoskeletal 5, Keratin-5, Review 009
Presentation	Biotin
Reactivity	Amph, Bov, Hu, Hydra, Ms, Por
Applications	C, E, F, ICC/IF, P
Clonality	Monoclonal
Clone	C22
Host	Mouse
Isotype	IgG1
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	Acris Antibodies GmbH

Datasheet Extract

Immunogen	Swiss Prot Num: P13647 Immunogen: Human keratin K8, purified from SDS PAGE gel Genename: 3852
Property	+ KRT8
Antibody type	Ab
Application	ELISA. Flow Cytometry. Immunocytology. Immunohistochemistry on Frozen Tissues: 1/10-1/20. Incubation time: 1 h at RT. Immunohistochemistry on Paraffin-Embedded Tissues: 1/10-1/20. Protease pretreatment is required prior to antibody application. Incubation time: Overnight at 2-8°C.
Background	Cytokeratin 5 is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. Cytokeratin 8 is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis.
General Readings	Bártek J, Vojtĕsek B, Stasková Z, Bártková J, Kerekés Z, Rejthar A, et al. A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology. J Pathol. 1991 Jul;164(3):215-24. PubMed PMID: 1716305. Heid H, Vojtesek B, Bruder G, Kaufman M, Staskova Z, Kovarik J, Bartek J, Franke WW: Cell type heterogeneity of mammary gland epithelial and breast carcinoma cells as revealed by cytokeratin expression. Differentiation (1991) Moll R, Franke WW, Schiller DL, Geiger B, Krepler R. The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells. Cell. 1982 Nov;31(1):11-24. PubMed PMID: 6186379. Waseem A, Karsten U, Leigh IM, Purkis P, Waseem NH, Lane EB. Conformational changes in the rod domain of human keratin 8 following heterotypic association with keratin 18 and its implication for filament stability. Biochemistry. 2004 Feb 10;43(5):1283-95. PubMed PMID: 14756564.
Storage	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Format	Label: Biotin Purification: Affinity Chromatography on Protein A Preservatives: 0.09% Sodium Azide State: Liquid purified IgG fraction
Specificity	Specificity: Antibody clone C22 represents an excellent marker for distinguishing carcinomas from all non-epithelial tumors. The antibody specifically reacts with Keratins K5 and K8 present in nearly all epithelia. Polypeptide Reacting: Mr 52 500, Mr 58 000 keratins (type II keratins K5 and K8; formerly also designated cytokeratins 5 and 8) of human epithelial cells. Reactivities on Cultured Cell Lines (tested so far): MCF-7, RT 112, HT-29, Detroit 562, RPMI 2650, SSC-12, bovine BMGE+H, BMGE-H, MDBK. Epitope has been mapped to aa 353-367 on alpha helical rod domain (See Waseem et al., 2004). Species: Human, Bovine, Murine, Amphibia, Pig and Hydra.
Gene ID	3852
FocusOn and Reviews	Reviews: Antibodies to Cytokeratin Proteins - Product Review 09
Targets	Cytokeratin 5 antibody

Accessory Products

Proteins and/or Positive Controls

Proteins for Cytokeratin 5 (2 products)

Catalog No.	Species	Source
H00003852-P01-10	Cytokeratin 5
	Human	in vitro transl.	10 µg / €350.00
			Abnova Taiwan Corp.
H00003852-P01-25	Cytokeratin 5
	Human	in vitro transl.	25 µg / €500.00
			Abnova Taiwan Corp.

Positive controls for Cytokeratin 5 (1 products)

Catalog No.	Species
NBL1-12393	Cytokeratin 5 Lysate
		0.1 mg / €370.00
		Novus Biologicals Inc.

Recommended Secondary Antibodies for Cytokeratin 5 (8 products)

Catalog No.	Host	Pres.
R1253AP	Mouse IgG (H+L chain) antibody
	Rabbit	AP
			Acris Antibodies GmbH
R1253B	Mouse IgG (H+L chain) antibody
	Rabbit	Biotin
			Acris Antibodies GmbH
R1253F	Mouse IgG (H+L chain) antibody
	Rabbit	FITC
			Acris Antibodies GmbH
R1253HRP	Mouse IgG (H+L chain) antibody
	Rabbit	HRP
			Acris Antibodies GmbH
R1253TR	Mouse IgG (H+L chain) antibody
	Rabbit	Texas Red
			Acris Antibodies GmbH
R1403R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Rabbit	PE
			Acris Antibodies GmbH
R1405R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Goat	PE
			Acris Antibodies GmbH
R1455R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Donkey	PE
			Acris Antibodies GmbH