Product Description for Cytokeratin 8
Mouse anti Human, Mouse Cytokeratin 8 5B3.
Properties: pSer431
Presentation: Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western blot / Immunoblot, Paraffin Sections.
Properties: pSer431
Presentation: Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western blot / Immunoblot, Paraffin Sections.
Properties for Cytokeratin 8
| Product Category | Antibodies |
|---|---|
| Quantity | 0.1 mg |
| Synonyms | CK8, CYK8, Cytokeratin endo A, Cytokeratin-8, K8, KRT8, Keratin, Keratin-8, Review 009, type II cytoskeletal 8 |
| Presentation | Purified |
| Reactivity | Hu, Ms |
| Applications | ICC/IF, IP, P, WB |
| Clonality | Monoclonal |
| Clone | 5B3 |
| Host | Mouse |
| Isotype | IgG1 |
| Shipping to | Worldwide |
| PDF datasheet | View Datasheet |
| Manufacturer | Acris Antibodies GmbH |
Datasheet Extract
| Immunogen |
|
|---|---|
| Property | pSer431 |
| Antibody type | Ab |
| Add. information | Mol. Wt. of Antigen: ~52.5kDa Cellular Localization: Cytoplasmic and cell membrane (3) |
| Application | Immunofluorescence (1). Western Blotting (1): 1-2 µg/ml for 2 hrs at RT. Immunoprecipitation: use Protein G, 2 µg/mg protein lysate. Immunohistology (Formalin/paraffin): 2-4 µg/ml for 60 min at RT, staining of formalin-fixed tissues REQUIRES boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Positive Control: Okadaic acid treated HT29 cells. Skin. |
| Background | Keratin 8 belongs to the type B (basic) subfamily of high molecular weight keratins and exists in combination with keratin 18 and other type I keratins. Keratin 8 is primarily found in the non-squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas. Hepatocellular carcinomas are defined by the use of antibodies that recognize only cytokeratin polypeptides 8 and 18. |
| Concentration | 0.2 mg/ml |
| General Readings |
|
| Storage | Store the antibody at 2 - 8 °C up to one month or in aliquots at -20 °C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| Format |
|
| Specificity |
|
| Gene ID | 3856 |
| FocusOn and Reviews | |
| Targets |
Accessory Products
Proteins and/or Positive Controls
Proteins for Cytokeratin 8 (8 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| AR09360PU-N |
Cytokeratin 8 |
||||||
 |
Human | Purified | > 95 % | E. coli |
0.25 mg /
€530.00
|
||
| Acris Antibodies GmbH | |||||||
| AR09360PU-S |
Cytokeratin 8 |
||||||
|
Human | Purified | > 95 % | E. coli |
0.1 mg /
€270.00
|
||
| Acris Antibodies GmbH | |||||||
| BA1007 |
Cytokeratin 8 |
||||||
 |
Bovine | Purified | > 95 % | Liver |
0.25 mg /
€380.00
|
||
| Acris Antibodies GmbH | |||||||
| BA1007S |
Cytokeratin 8 |
||||||
|
Bovine | Purified | > 95 % | Liver |
0.1 mg /
€250.00
|
||
| Acris Antibodies GmbH | |||||||
| H00003856-P01-10 |
Cytokeratin 8 |
||||||
|
Human | Purified |
10 µg /
€350.00
|
||||
| Abnova Taiwan Corp. | |||||||
| H00003856-P01-25 |
Cytokeratin 8 |
||||||
|
Human | Purified |
25 µg /
€500.00
|
||||
| Abnova Taiwan Corp. | |||||||
| H00003856-Q01-10 |
Cytokeratin 8 |
||||||
|
Human | in vitro transl. |
10 µg /
€350.00
|
||||
| Abnova Taiwan Corp. | |||||||
| H00003856-Q01-25 |
Cytokeratin 8 |
||||||
|
Human | in vitro transl. |
25 µg /
€500.00
|
||||
| Abnova Taiwan Corp. | |||||||
Positive controls for Cytokeratin 8 (3 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| H00003856-T01 |
Cytokeratin-8 (KRT8) Lysate(Denatured) |
||||||
|
0.1 ml /
€240.00
|
||||||
| Abnova Taiwan Corp. | |||||||
| H00003856-T02 |
Cytokeratin-8 (KRT8) Lysate(Denatured) |
||||||
|
0.1 ml /
€240.00
|
||||||
| Abnova Taiwan Corp. | |||||||
| NBL1-12397 |
Cytokeratin 8 Lysate |
||||||
![Western Blot: Cytokeratin 8 Lysate [NBL1-12397] - Western Blot experiments.
Left-Control; Right -Over-expression Lysate for KRT8](http://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/Novus/Cytokeratin 8 Lysate-Western Blot-NBL1-12397-img0001.jpg) |
0.1 mg /
€370.00
|
||||||
| Novus Biologicals Inc. | |||||||
Recommended Secondary Antibodies for Cytokeratin 8 (8 products)
| Catalog No. | Host | Clone/Iso. | Pres. | React. | Applications | ||
|---|---|---|---|---|---|---|---|
| R1253AP |
Mouse IgG (H+L chain) antibody |
||||||
|
|
Rabbit | AP | |||||
| Acris Antibodies GmbH | |||||||
| R1253B |
Mouse IgG (H+L chain) antibody |
||||||
|
|
Rabbit | Biotin | |||||
| Acris Antibodies GmbH | |||||||
| R1253F |
Mouse IgG (H+L chain) antibody |
||||||
|
|
Rabbit | FITC | |||||
| Acris Antibodies GmbH | |||||||
| R1253HRP |
Mouse IgG (H+L chain) antibody |
||||||
|
Rabbit | HRP | |||||
| Acris Antibodies GmbH | |||||||
| R1253TR |
Mouse IgG (H+L chain) antibody |
||||||
|
|
Rabbit | Texas Red | |||||
| Acris Antibodies GmbH | |||||||
| R1403R |
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
||||||
|
|
Rabbit | PE | |||||
| Acris Antibodies GmbH | |||||||
| R1405R |
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
||||||
|
|
Goat | PE | |||||
| Acris Antibodies GmbH | |||||||
| R1455R |
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
||||||
|
|
Donkey | PE | |||||
| Acris Antibodies GmbH | |||||||
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