AM10027PU-S Cytokeratin 8 (+ KRT18) antibody

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0.1 mg / €190.00
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Mouse anti Human Cytokeratin 8 CK207

AM10027PU-S

Product Description for Cytokeratin 8

Mouse anti Human Cytokeratin 8 CK207.
Properties: (+ KRT18)
Presentation: Purified
Product is tested for Frozen Sections, Immunocytochemistry/Immunofluorescence, Paraffin Sections.

Properties for Cytokeratin 8

Product Category Antibodies
Quantity 0.1 mg
Synonyms CK8, CYK8, Cytokeratin endo A, Cytokeratin-8, K8, KRT8, Keratin, Keratin-8, Review 009, type II cytoskeletal 8
Presentation Purified
Reactivity Hu
Applications C, ICC/IF, P
Clonality Monoclonal
Clone CK207
Host Mouse
Isotype IgG2a
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH

Datasheet Extract

Immunogen
Swiss Prot Num:
P05787
Immunogen:
Purified keratins.
Genename:
3856
Remarks:
Molecular weight of antigen: CK8 is 52.5 kDa and CK18 is 45 kDa.
Property + KRT18
Antibody type Ab
Application Suitable for Immunohistochemistry and Immunocytochemistry (Frozen or Formalin-Fixed Paraffin-Embedded (FFPE) tissue sections and cell smears)
For IHC dilute concentared antibody at 1/50-1/100, use streptavidin~biotin system or polymer system, incubate 30 minutes at room temperature.
Immunofluorescence.
Recommended Positive Control: Human breast, any GI tissue or skin.
Background Cytokeratins are intermediate filament keratins found in the intracytoplasmic cytoskeleton of epithelial tissue There are two types of Cytokeratins: the low weight, acidic type I cytokeratins and the high weight, basic or neutral type II. Cytokeratins are usually found in pairs comprising a type I Cytokeratin and a type II cytokeratin. The high molecular weight cytokeratins, which are the basic or neutral cytokeratins, comprise subtypes CK1, CK2, CK3, CK4, CK5, CK6, CK7, CK8 and CK9. The low molecular weight cytokeratins, which are the acidic cytokeratins, comprise subtypes CK10, CK12, CK 13, CK14, CK16, CK17, CK18, CK19 and CK20.
Concentration 0.2 mg/ml
General Readings
  • Moll, R et al Cell, 31: 11-24, (1982).
  • Angus B et. al. J Path., 155: 71-75, 1988.
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
Format
Purification:
Protein A Chromatography.
Buffer System:
PBS, pH 7.4 containing 1% BSA as stabilizer and 0.05% Sodium Azide as preservative.
State:
Liquid purified Ig fraction.
Purified
Specificity
Specificity:
This antibody recognizes all simple epithelia and glandular (thyroid, breast, GI tract, respiratory tract, urinary tract).
Most squamous carcinomas and all adenocarcinomas are stained by this antibody and keratinizing squamous carcinomas are mostly negative.
Cellular Localization: Cytoplasmic.
Species:
Human.
Gene ID 3856
FocusOn and Reviews
Reviews:
Targets

Accessory Products

Proteins and/or Positive Controls

Proteins for Cytokeratin 8 (8 products)

Catalog No. Species Pres. Purity   Source  
AR09360PU-N

Cytokeratin 8

Cytokeratin 8 Human Purified > 95 % E. coli
0.25 mg / €530.00
  Acris Antibodies GmbH
AR09360PU-S

Cytokeratin 8

Cytokeratin 8 Human Purified > 95 % E. coli
0.1 mg / €270.00
  Acris Antibodies GmbH
BA1007

Cytokeratin 8

Lane 1: Phosphorylase B (a), BSA (b), Ovalbumin (c), Carbonic Anhydrase (d). Lane 2: keratin K8 Bovine Purified > 95 % Liver
0.25 mg / €380.00
  Acris Antibodies GmbH
BA1007S

Cytokeratin 8

Lane 1: Phosphorylase B (a), BSA (b), Ovalbumin (c), Carbonic Anhydrase (d). Lane 2: keratin K8 Bovine Purified > 95 % Liver
0.1 mg / €250.00
  Acris Antibodies GmbH
H00003856-P01-10

Cytokeratin 8

Cytokeratin 8 Human Purified
10 µg / €350.00
  Abnova Taiwan Corp.
H00003856-P01-25

Cytokeratin 8

Cytokeratin 8 Human Purified
25 µg / €500.00
  Abnova Taiwan Corp.
H00003856-Q01-10

Cytokeratin 8

Cytokeratin 8 Human in vitro transl.
10 µg / €350.00
  Abnova Taiwan Corp.
H00003856-Q01-25

Cytokeratin 8

Cytokeratin 8 Human in vitro transl.
25 µg / €500.00
  Abnova Taiwan Corp.

Positive controls for Cytokeratin 8 (3 products)

Catalog No. Species Pres. Purity   Source  
H00003856-T01

Cytokeratin-8 (KRT8) Lysate(Denatured)

Cytokeratin-8 (KRT8) Lysate(Denatured)
0.1 ml / €240.00
  Abnova Taiwan Corp.
H00003856-T02

Cytokeratin-8 (KRT8) Lysate(Denatured)

Cytokeratin-8 (KRT8) Lysate(Denatured)
0.1 ml / €240.00
  Abnova Taiwan Corp.
NBL1-12397

Cytokeratin 8 Lysate

Western Blot: Cytokeratin 8 Lysate [NBL1-12397] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for KRT8
0.1 mg / €370.00
  Novus Biologicals Inc.