Cytokeratin 8

Product Description for Cytokeratin 8

Mouse anti Hamster, Human, Mouse, Rat Cytokeratin 8 Ks8.7.
Presentation: Purified
Product is tested for Frozen Sections, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot, Paraffin Sections.

Properties for Cytokeratin 8

Product Category	Antibodies
Quantity	50 µg
Synonyms	CK8, CYK8, Cytokeratin endo A, Cytokeratin-8, K8, KRT8, Keratin, Keratin-8, Review 009, type II cytoskeletal 8
Presentation	Purified
Reactivity	Hst, Hu, Ms, Rt
Applications	C, ICC/IF, P, WB
Clonality	Monoclonal
Clone	Ks8.7
Host	Mouse
Isotype	IgG1
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	Acris Antibodies GmbH

Datasheet Extract

Immunogen	Swiss Prot Num: P05787 Immunogen: Cytoskeletal proteins from cultured HeLa cells Genename: 3856
Antibody type	Ab
Application	Western blot. Immunocytology. Immunohistochemistry (1:10) on frozen tissues and paraffin-embedded sections after microwave treatment. Incubation time: 1 h at RT.
Background	Cytokeratin 8 belongs to the type B (basic) subfamily of high molecular weight keratins and exists in combination with cytokeratin 18. Cytokeratin 8 is primarily found in the non squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas. Hepatocellular carcinomas are defined by the use of antibodies that recognize only cytokeratin polypeptides 8 and 18.
General Readings	Bártek J, Vojtĕsek B, Stasková Z, Bártková J, Kerekés Z, Rejthar A, et al. A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology. J Pathol. 1991 Jul;164(3):215-24. PubMed PMID: 1716305. Moll R, Franke WW, Schiller DL, Geiger B, Krepler R. The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells. Cell. 1982 Nov;31(1):11-24. PubMed PMID: 6186379. Osborn M, van Lessen G, Weber K, Klöppel G, Altmannsberger M: Methods in Laboratory Investigation. Differential diagnosis of gastrointestinal carconomas by using monoclonal antibodies specific for individual keratin polypeptides. Lab. Investigation 55,4, 497-504 (1986) Gomi H, Yokoyama T, Fujimoto K, Ikeda T, Katoh A, Itoh T, et al. Mice devoid of the glial fibrillary acidic protein develop normally and are susceptible to scrapie prions. Neuron. 1995 Jan;14(1):29-41. PubMed PMID: 7826639. Stumptner C, Fuchsbichler A, Heid H, Zatloukal K, Denk H: Mallory body - a disease-associated type of sequestosome. Hepatology 35, 1053-1062 (2002)
Storage	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Format	Purification: Protein A affinity chromatography Buffer System: Final solution contains 0.09 % sodium azide and 0.5% BSA in PBS buffer, pH 7.4. State: Lyophilized purified IgG Purified Reconstitution: Restore in 1 ml distilled water.
Specificity	Specificity: Ks 8.7 represents an excellent marker to discriminate simple epithelia from those of different origin. Polypetide reacting: Mr 52500 polypeptide (keratin K8; formerly also designated cytokeratin 8) of human epithelia. Tumors specifically detected: All adeno-CA tested; undifferentiated CA; cervix CA; hepatocellular CA. Reactivities on cultured cell lines (tested so far): MCF-7, RT 112, PLC. Species: Human, mouse, rat, Tupaia belangeri, Monodelphis domestica, hamster. Negative with bovine and pig.
Gene ID	3856
FocusOn and Reviews	Reviews: Antibodies to Cytokeratin Proteins - Product Review 09
Targets	Cytokeratin 8 antibody

Accessory Products

Proteins and/or Positive Controls

Proteins for Cytokeratin 8 (8 products)

Catalog No.	Species	Pres.	Purity	Source
AR09360PU-N	Cytokeratin 8
	Human	Purified	> 95 %	E. coli	0.25 mg / €530.00
					Acris Antibodies GmbH
AR09360PU-S	Cytokeratin 8
	Human	Purified	> 95 %	E. coli	0.1 mg / €270.00
					Acris Antibodies GmbH
BA1007	Cytokeratin 8
	Bovine	Purified	> 95 %	Liver	0.25 mg / €380.00
					Acris Antibodies GmbH
BA1007S	Cytokeratin 8
	Bovine	Purified	> 95 %	Liver	0.1 mg / €250.00
					Acris Antibodies GmbH
H00003856-P01-10	Cytokeratin 8
	Human	Purified			10 µg / €350.00
					Abnova Taiwan Corp.
H00003856-P01-25	Cytokeratin 8
	Human	Purified			25 µg / €500.00
					Abnova Taiwan Corp.
H00003856-Q01-10	Cytokeratin 8
	Human			in vitro transl.	10 µg / €350.00
					Abnova Taiwan Corp.
H00003856-Q01-25	Cytokeratin 8
	Human			in vitro transl.	25 µg / €500.00
					Abnova Taiwan Corp.

Positive controls for Cytokeratin 8 (3 products)

Catalog No.	Species
H00003856-T01	Cytokeratin-8 (KRT8) Lysate(Denatured)
		0.1 ml / €240.00
		Abnova Taiwan Corp.
H00003856-T02	Cytokeratin-8 (KRT8) Lysate(Denatured)
		0.1 ml / €240.00
		Abnova Taiwan Corp.
NBL1-12397	Cytokeratin 8 Lysate
		0.1 mg / €370.00
		Novus Biologicals Inc.

Recommended Secondary Antibodies for Cytokeratin 8 (8 products)

Catalog No.	Host	Pres.
R1253AP	Mouse IgG (H+L chain) antibody
	Rabbit	AP
			Acris Antibodies GmbH
R1253B	Mouse IgG (H+L chain) antibody
	Rabbit	Biotin
			Acris Antibodies GmbH
R1253F	Mouse IgG (H+L chain) antibody
	Rabbit	FITC
			Acris Antibodies GmbH
R1253HRP	Mouse IgG (H+L chain) antibody
	Rabbit	HRP
			Acris Antibodies GmbH
R1253TR	Mouse IgG (H+L chain) antibody
	Rabbit	Texas Red
			Acris Antibodies GmbH
R1403R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Rabbit	PE
			Acris Antibodies GmbH
R1405R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Goat	PE
			Acris Antibodies GmbH
R1455R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Donkey	PE
			Acris Antibodies GmbH