DM189 Cytokeratin 8 (+ KRT18) antibody

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1 ml / €330.00
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Quick Overview

Mouse anti Human Cytokeratin 8 5D3

DM189

Product Description for Cytokeratin 8

Mouse anti Human Cytokeratin 8 5D3.
Properties: (+ KRT18)
Presentation: Ascites
Product is tested for Frozen Sections, Western blot / Immunoblot, Paraffin Sections.

Properties for Cytokeratin 8

Product Category Antibodies
Target Category
Quantity 1 ml
Synonyms CK8, CYK8, Cytokeratin endo A, Cytokeratin-8, K8, KRT8, Keratin, Keratin-8, type II cytoskeletal 8
Presentation Ascites
Reactivity Hu
Applications C, P, WB
Clonality Monoclonal
Clone 5D3
Host Mouse
Isotype IgG2a
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P05787
Immunogen:
Isolated from human breast cancer carcinoma cell line MCF-7
Genename:
3856
Remarks:
Epitope has been mapped to aa 353-367 on alpha helical rod domain of keratin K8.
Property + KRT18
Antibody type Ab
Application Cell Suspensions.
Immunohistochemistry on Frozen Sections.
Immunohistochemistry on Paraffin Sections.

(After Microwave treatment, Proteolytic treatment optional).
Immunoblotting (Western): NCL5D3 is directed against the the 52.2 kD keratin K8 (originally reported also to 45 kD keratin K18; formerly also designated cytokeratins according to the Moll-catalogue).
Working Dilution: 1/5-1/10, dilute immediately before use.
Dilution Buffer: PBS with 1% BSA and 0.09% Sodium Azide.
Recommended Positive Control: Glandular epithelium.
Background Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 – 7.8. The individual cytokeratin polypeptides are numbered 1 to 20.
The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium.
Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
Cytokeratin 8 belongs to the type B (basic) subfamily of high molecular weight keratins and exists in combination with Cytokeratin 18 (type A; acidic subfamily of low molecular weight keratins).
General Readings
  1. Angus B, Purvis J, Stock D, Westley BR, Samson AC, Routledge EG, et al. NCL-5D3: a new monoclonal antibody recognizing low molecular weight cytokeratins effective for immunohistochemistry using fixed paraffin-embedded tissue. J Pathol. 1987 Dec;153(4):377-84. PubMed PMID: 2448441.
  2. Waseem A, Karsten U, Leigh IM, Purkis P, Waseem NH, Lane EB. Conformational changes in the rod domain of human keratin 8 following heterotypic association with keratin 18 and its implication for filament stability. Biochemistry. 2004 Feb 10;43(5):1283-95. PubMed PMID: 14756564.
  3. Angus B, Kiberu S, Purvis J, Wilkinson L, Horne CH. Cytokeratins in cervical dysplasia and neoplasia: a comparative study of immunohistochemical staining using monoclonal antibodies NCL-5D3, CAM 5.2, and PKK1. J Pathol. 1988 May;155(1):71-5. PubMed PMID: 2454306.
  4. Kasper M, Rudolf T, Verhofstad AA, Schuh D, Müller M. Heterogeneity in the immunolocalization of cytokeratin-specific monoclonal antibodies in the rat lung: evaluation of three different alveolar epithelial cell types. Histochemistry. 1993 Jul;100(1):65-71. PubMed PMID: 7693628.
  5. Martín CA, Salomoni PD, Badrán AF. Cytokeratin immunoreactivity in mouse tissues: study of different antibodies with a new detection system. Appl Immunohistochem Mol Morphol. 2001 Mar;9(1):70-3. PubMed PMID: 11277418.
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Preservatives:
0.09% Sodium Azide
State:
Ascites
Ascites
Species Reactivity
Species reactivity (tested):
Human.No significant cross-reaction with Mouse and Rat.
Specificity
Specificity:
The antibody recognizes all simple epithelia including glandular epithelia.
It does not react with stratified squamous epithelium.
Useful for the differentiation between carcinomas and lymphomas.
Gene ID 3856
FocusOn and Reviews
Reviews:

Accessory Products

Proteins and/or Positive Controls

Proteins for Cytokeratin 8 (10 products)

Catalog No. Species Pres. Purity   Source  
AR09360PU-N

Cytokeratin 8

Cytokeratin 8 Human Purified > 95 % determined by SDS gelelectrophoresis E. coli
0.25 mg / €530.00
  Acris Antibodies GmbH
AR09360PU-S

Cytokeratin 8

Cytokeratin 8 Human Purified > 95 % determined by SDS gelelectrophoresis E. coli
0.1 mg / €270.00
  Acris Antibodies GmbH
AR50900PU-N

Cytokeratin 8 (1-483, His-tag)

Cytokeratin 8 Human Purified > 90 % by SDS - PAGE E. coli
0.5 mg / €730.00
  Acris Antibodies GmbH
AR50900PU-S

Cytokeratin 8 (1-483, His-tag)

Cytokeratin 8 Human Purified > 90 % by SDS - PAGE E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH
BA1007

Cytokeratin 8

Lane 1: Phosphorylase B (a), BSA (b), Ovalbumin (c), Carbonic Anhydrase (d). Lane 2: keratin K8 Bovine Purified > 95 % (determined by SDS gelelectrophoresis) Liver
0.25 mg / €380.00
  Acris Antibodies GmbH
BA1007S

Cytokeratin 8

Lane 1: Phosphorylase B (a), BSA (b), Ovalbumin (c), Carbonic Anhydrase (d). Lane 2: keratin K8 Bovine Purified > 95 % (determined by SDS gelelectrophoresis) Liver
0.1 mg / €250.00
  Acris Antibodies GmbH
H00003856-P01-10

Cytokeratin 8

Cytokeratin 8 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €350.00
  Abnova Taiwan Corp.
H00003856-P01-25

Cytokeratin 8

Cytokeratin 8 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €500.00
  Abnova Taiwan Corp.
H00003856-Q01-10

Cytokeratin 8

Cytokeratin 8 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €350.00
  Abnova Taiwan Corp.
H00003856-Q01-25

Cytokeratin 8

Cytokeratin 8 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €500.00
  Abnova Taiwan Corp.

Positive controls for Cytokeratin 8 (3 products)

Catalog No. Species Pres. Purity   Source  
H00003856-T01

Cytokeratin-8 (KRT8) Lysate(Denatured)

Cytokeratin-8 (KRT8) Lysate(Denatured)
0.1 ml / €240.00
  Abnova Taiwan Corp.
H00003856-T02

Cytokeratin-8 (KRT8) Lysate(Denatured)

Cytokeratin-8 (KRT8) Lysate(Denatured)
0.1 ml / €240.00
  Abnova Taiwan Corp.
NBL1-12397

Cytokeratin 8 Lysate

Western Blot: Cytokeratin 8 Lysate [NBL1-12397] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for KRT8
0.1 mg / €370.00
  Novus Biologicals Inc.
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