Product Description for Cytokeratin 8
Mouse anti Bovine, Human, Porcine, Rabbit, Sheep Cytokeratin 8 C-43.
Presentation: Aff - Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western blot / Immunoblot, Paraffin Sections.
Presentation: Aff - Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western blot / Immunoblot, Paraffin Sections.
Properties for Cytokeratin 8
| Product Category | Antibodies |
|---|---|
| Quantity | 0.1 mg |
| Synonyms | CK8, CYK8, Cytokeratin endo A, Cytokeratin-8, K8, KRT8, Keratin, Keratin-8, Review 009, type II cytoskeletal 8 |
| Presentation | Aff - Purified |
| Reactivity | Bov, Hu, Por, Rb, Sh |
| Applications | ICC/IF, IP, P, WB |
| Clonality | Monoclonal |
| Clone | C-43 |
| Host | Mouse |
| Isotype | IgG1 |
| Shipping to | Worldwide |
| PDF datasheet | View Datasheet |
| Manufacturer | Acris Antibodies GmbH |
Datasheet Extract
| Immunogen |
|
|---|---|
| Antibody type | Ab |
| Application | Western Blot. Immunoprecipitation. Immunocytochemistry. Immunohistochemistry on Paraffin Sections: 20 µg/ml. Heat induced antigen retrieval in pH 6.0 citrate buffer is recommended. Positive Tissue: Prostate. |
| Background | Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families. Cytokeratin 8 belongs to the type B (basic) subfamily of high molecular weight keratins and exists in combination with cytokeratin 18. Cytokeratin 8 is primarily found in the non squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas. Hepatocellular carcinomas are defined by the use of antibodies that recognize only cytokeratin polypeptides 8 and 18. |
| Concentration | 1.0 mg/ml |
| General Readings |
|
| Storage | Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| Format |
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| Specificity |
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| Gene ID | 3856 |
| FocusOn and Reviews | |
| Targets |
Accessory Products
Proteins and/or Positive Controls
Proteins for Cytokeratin 8 (8 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| AR09360PU-N |
Cytokeratin 8 |
||||||
 |
Human | Purified | > 95 % | E. coli |
0.25 mg /
€530.00
|
||
| Acris Antibodies GmbH | |||||||
| AR09360PU-S |
Cytokeratin 8 |
||||||
|
Human | Purified | > 95 % | E. coli |
0.1 mg /
€270.00
|
||
| Acris Antibodies GmbH | |||||||
| BA1007 |
Cytokeratin 8 |
||||||
 |
Bovine | Purified | > 95 % | Liver |
0.25 mg /
€380.00
|
||
| Acris Antibodies GmbH | |||||||
| BA1007S |
Cytokeratin 8 |
||||||
|
Bovine | Purified | > 95 % | Liver |
0.1 mg /
€250.00
|
||
| Acris Antibodies GmbH | |||||||
| H00003856-P01-10 |
Cytokeratin 8 |
||||||
|
Human | Purified |
10 µg /
€350.00
|
||||
| Abnova Taiwan Corp. | |||||||
| H00003856-P01-25 |
Cytokeratin 8 |
||||||
|
Human | Purified |
25 µg /
€500.00
|
||||
| Abnova Taiwan Corp. | |||||||
| H00003856-Q01-10 |
Cytokeratin 8 |
||||||
|
Human | in vitro transl. |
10 µg /
€350.00
|
||||
| Abnova Taiwan Corp. | |||||||
| H00003856-Q01-25 |
Cytokeratin 8 |
||||||
|
Human | in vitro transl. |
25 µg /
€500.00
|
||||
| Abnova Taiwan Corp. | |||||||
Positive controls for Cytokeratin 8 (3 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| H00003856-T01 |
Cytokeratin-8 (KRT8) Lysate(Denatured) |
||||||
|
0.1 ml /
€240.00
|
||||||
| Abnova Taiwan Corp. | |||||||
| H00003856-T02 |
Cytokeratin-8 (KRT8) Lysate(Denatured) |
||||||
|
0.1 ml /
€240.00
|
||||||
| Abnova Taiwan Corp. | |||||||
| NBL1-12397 |
Cytokeratin 8 Lysate |
||||||
![Western Blot: Cytokeratin 8 Lysate [NBL1-12397] - Western Blot experiments.
Left-Control; Right -Over-expression Lysate for KRT8](http://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/Novus/Cytokeratin 8 Lysate-Western Blot-NBL1-12397-img0001.jpg) |
0.1 mg /
€370.00
|
||||||
| Novus Biologicals Inc. | |||||||
Recommended Secondary Antibodies for Cytokeratin 8 (8 products)
| Catalog No. | Host | Clone/Iso. | Pres. | React. | Applications | ||
|---|---|---|---|---|---|---|---|
| R1253AP |
Mouse IgG (H+L chain) antibody |
||||||
|
|
Rabbit | AP | |||||
| Acris Antibodies GmbH | |||||||
| R1253B |
Mouse IgG (H+L chain) antibody |
||||||
|
|
Rabbit | Biotin | |||||
| Acris Antibodies GmbH | |||||||
| R1253F |
Mouse IgG (H+L chain) antibody |
||||||
|
|
Rabbit | FITC | |||||
| Acris Antibodies GmbH | |||||||
| R1253HRP |
Mouse IgG (H+L chain) antibody |
||||||
|
Rabbit | HRP | |||||
| Acris Antibodies GmbH | |||||||
| R1253TR |
Mouse IgG (H+L chain) antibody |
||||||
|
|
Rabbit | Texas Red | |||||
| Acris Antibodies GmbH | |||||||
| R1403R |
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
||||||
|
|
Rabbit | PE | |||||
| Acris Antibodies GmbH | |||||||
| R1405R |
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
||||||
|
|
Goat | PE | |||||
| Acris Antibodies GmbH | |||||||
| R1455R |
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
||||||
|
|
Donkey | PE | |||||
| Acris Antibodies GmbH | |||||||
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