DM3522P CD309 / VEGFR-2 / Flk-1 antibody

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0.1 mg / €320.00
Please visit the appropriate website of Acris Antibodies or contact your local Distributor to buy this product.

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Mouse anti Human CD309 / VEGFR-2 / Flk-1 4H3

DM3522P

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Product Description for CD309 / VEGFR-2 / Flk-1

Mouse anti Human CD309 / VEGFR-2 / Flk-1 4H3.
Presentation: Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Enzyme Immunoassay, Western blot / Immunoblot, Flow Cytometry.

Properties for CD309 / VEGFR-2 / Flk-1

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms FLK1, KDR, Kinase NYK, Kinase insert domain receptor, Protein-tyrosine kinase receptor Flk-1, VEGF Receptor 2, VEGFR2, Vascular endothelial growth factor receptor 2
Presentation Purified
Reactivity Hu
Applications E, F, ICC/IF, WB
Clonality Monoclonal
Clone 4H3
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P35968
Immunogen:
Recombinant Human soluble extracellular KDR (D7) (110 kDa) protein (Cat.-No AR26018PU-N)
Genename:
3791
Isotype control AM03095PU-N, SM10P (for use in human samples)
Application ELISA: 1-15 µg/ml.
Western blot: 2-5 µg/ml.  
Immunofluorescence/Immunohistochemistry: 1-5 μg/ml. 
Flow Cytometry and Cell Sorting: Use at 2-5 µg/ml together with the appropriate secondary reagents.
Background VEGF receptor 2 is a member of a receptor tyrosine kinase family whose activation plays an essential role in a large number of biological processes such as embryonic development, wound healing, cell proliferation, migration and differentiation. Like other growth factor receptors, upon ligand binding VEGF receptor 2 dimerises and is autophosphorylated on multiple tyrosine residues. These sites can be involved in the regulation of kinase activity or serve as binding sites for SH2 and phosphotyrosine binding containing signalling proteins. Phosphorylation of Tyrosines 1054 and 1059 in the activation loop is required for activation of VEGF receptor 2 and its intrinsic tyrosine kinase activity. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
General Readings
  1. Shibata et al, BMC Medicine 8 (2010)
  2. Albaquerque et al, Nature Med 2009
  3. Ebos et al, Mol Cancer Res 2 (2004)
  4. Ebos et al, Cancer res 68 (2008).
  5. Benzinger et al., BBA 1466:71, 2000.
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month 
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Protein G Chromatography
Buffer System:
PBS pH 7.4 without preservatives
State:
Lyophilized purified IgG fraction
Purified
Reconstitution:
Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
Species Reactivity
Species reactivity (tested):
Human.
Specificity
Specificity:
This antibody will detect native Human VEGFR-2/KDR in ELISA experiments and on the surface of different Human cell types.
Gene ID 3791
FocusOn and Reviews
FocusOns:

Accessory Products

Proteins and/or Positive Controls

Proteins for CD309 / VEGFR-2 / Flk-1 (8 products)

Catalog No. Species Pres. Purity   Source  
AR26018PU-L

CD309 / VEGFR-2 / Flk-1 (esKDR)

SDS-PAGE analysis of recombinant endogenous esKDR produced in insect cells. Sample was loaded in 10% SDS-polyacrylamide gel under reducing conditions and stained with Silver staining. Human Purified > 95 % by SDS-PAGE and silver stain Insect cells
20 µg / €430.00
  Acris Antibodies GmbH
AR26018PU-N

CD309 / VEGFR-2 / Flk-1 (esKDR)

SDS-PAGE analysis of recombinant endogenous esKDR produced in insect cells. Sample was loaded in 10% SDS-polyacrylamide gel under reducing conditions and stained with Silver staining. Human Purified > 95 % by SDS-PAGE and silver stain Insect cells
5 µg / €210.00
  Acris Antibodies GmbH
DA3530

CD309 / VEGFR-2 / Flk-1 (D1-D7)

Primary human umbilical vein endothelial (HUVE) cells were stimulated with 10ng/ml VEGF165 which was preincubated with an 100-fold excess of recombinant human soluble VEGFR-2/KDR receptors. Stimulation with bFGF (basic) was used a second control. Human Purified > 95 % pure as by SDS-PAGE and silver stain. Insect cells
5 µg / €130.00
  Acris Antibodies GmbH
DA3530X

CD309 / VEGFR-2 / Flk-1 (D1-D7)

Primary human umbilical vein endothelial (HUVE) cells were stimulated with 10ng/ml VEGF165 which was preincubated with an 100-fold excess of recombinant human soluble VEGFR-2/KDR receptors. Stimulation with bFGF (basic) was used a second control. Human Purified > 90 % pure as by SDS-PAGE and silver stain. Insect cells
50 µg / €360.00
  Acris Antibodies GmbH
DA3531

CD309 / VEGFR-2 / Flk-1 (D1-7) (Fc Chimera)

Inhibition of the VEGF165-induced proliferation of HUVE cells by recombinant human and mouse sKDR(D7)-Fc and sFlk-1(D7)-Fc. HUVECs were stimulated with 10 ng/ml VEGF165, both soluble receptors were added with a 100X excess. Human Purified > 90 % by SDS-PAGE and visualised by silver stain. Insect cells
50 µg / €280.00
  Acris Antibodies GmbH
DA3531S

CD309 / VEGFR-2 / Flk-1 (D1-7) (Fc Chimera)

Inhibition of the VEGF165-induced proliferation of HUVE cells by recombinant human and mouse sKDR(D7)-Fc and sFlk-1(D7)-Fc. HUVECs were stimulated with 10 ng/ml VEGF165, both soluble receptors were added with a 100X excess. Human Purified > 90 % by SDS-PAGE and visualised by silver stain. Insect cells
10 µg / €150.00
  Acris Antibodies GmbH
H00003791-Q01-10

CD309 / VEGFR-2 / Flk-1

CD309 / VEGFR-2 / Flk-1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €350.00
  Abnova Taiwan Corp.
H00003791-Q01-25

CD309 / VEGFR-2 / Flk-1

CD309 / VEGFR-2 / Flk-1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €500.00
  Abnova Taiwan Corp.

Positive controls for CD309 / VEGFR-2 / Flk-1 (1 products)

Catalog No. Species Pres. Purity   Source  
NBL1-06701

VEGF Receptor 2 Lysate

Western Blot: VEGFR1/VEGFR2 Antibody [NB100-527] - Western Blot on VEGFR2 overexpression lysates.
0.1 ml / €170.00
  Novus Biologicals Inc.
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