Estrogen receptor alpha (C-term) - AM11134PU-N | acris-antibodies.com

JavaScript seems to be disabled in your browser.
You must have JavaScript enabled in your browser to utilize the functionality of this website.

Home » Antibodies » Estrogen receptor alpha

Product Description for Estrogen receptor alpha

Rabbit anti Hamster, Human Estrogen receptor alpha SP1.
Properties: (C-term)
Presentation: Supernatant
Product is tested for Paraffin Sections, Western blot / Immunoblot.

Properties for Estrogen receptor alpha

Product Category	Antibodies
Quantity	1 ml
Synonyms	ER alpha, ESR, ESR1, Estradiol receptor, FocusOn050, FocusOn098, NR3A1, Nuclear receptor subfamily 3 group A member 1
Presentation	Supernatant
Reactivity	Hst, Hu
Applications	P, WB
Clonality	Monoclonal
Clone	SP1
Host	Rabbit
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	Acris Antibodies GmbH

Datasheet Extract

Immunogen	Swiss Prot Num: P03372 Immunogen: Synthetic peptide derived from C-terminal of Human Estrogen Receptor alpha. Genename: 2099 Remarks: Epitope: C-terminus. Molecular Weight: 67kDa.
Property	C-term
Antibody type	Ab
Application	Western Blot: 1/25, incubate for 1 hour at RT. Immunohistochemistry with Formalin-Fixed, Paraffin-Embedded tissues: 1/200 Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Incubation Time: 30 minutes at RT. Positive Control: Breast carcinoma.
Background	Steroid hormone receptors exhibit a high affinity and specificity for their ligands. The human estrogen receptor (ER) exists in two forms, ERalpha and ERbeta. The receptors have a high degree of homology in their DNA binding and ligand binding domains and can form heterodimers. However, the two ERs signal in different ways; for example, ERbeta does not potentiate the nuclear transcription factor AP-1 when complexed with oestradiol, whereas ERalpha does. This suggests that ERalpha and ERbeta may have different roles in gene regulation and their relative levels within tissues may influence cellular responses to oestrogens.
General Readings	Rocha R, Nunes C, Rocha G, Oliveira F, Sanches F, Gobbi H. Rabbit monoclonal antibodies show higher sensitivity than mouse monoclonals for estrogen and progesterone receptor evaluation in breast cancer by immunohistochemistry. Pathol Res Pract. 2008;204(9):655-62. doi: 10.1016/j.prp.2008.03.010. Epub 2008 Jun 18. PubMed PMID: 18565685. Cheang MC, Treaba DO, Speers CH, Olivotto IA, Bajdik CD, Chia SK, et al. Immunohistochemical detection using the new rabbit monoclonal antibody SP1 of estrogen receptor in breast cancer is superior to mouse monoclonal antibody 1D5 in predicting survival. J Clin Oncol. 2006 Dec 20;24(36):5637-44. Epub 2006 Nov 20. PubMed PMID: 17116944. Huang Z, Zhu W, Szekeres G, Xia H. Development of new rabbit monoclonal antibody to estrogen receptor: immunohistochemical assessment on formalin-fixed, paraffin-embedded tissue sections. Appl Immunohistochem Mol Morphol. 2005 Mar;13(1):91-5. PubMed PMID: 15722800.
Storage	Store the antibody undiluted at 2-8°C. DO NOT FREEZE! Shelf life: one year from despatch.
Format	Buffer System: TBS buffer, pH7,5 containing 1% BSA as stabilizer and 0.09% Sodium Azide as preservative. State: Liquid Tissue Culture Supernatant Supernatant
Species Reactivity	Species reactivity (expected): Hamster. Species reactivity (tested): Human.
Specificity	Specificity: This monoclonal anti-Human Estrogen Receptor antibody (Clone SP1) is intended for the qualitative detection of estrogen receptor (ER) alpha form in sections of formalin-fixed, paraffin embedded tissues. This antibody recognizes a protein of 67kDa which is identified as estrogen receptor (ER). This antibody strongly stains the nucleus of epithelial cells in breast carcinomas. The ER is an important regulator of growth and differentiation in the mammary gland. Steroid hormone receptors exhibit a high affinity and specificity for their ligands. Cellular Localization: Nucleus.
Gene ID	2099
FocusOn and Reviews	FocusOns: Organelle Markers: Antibodies to the Nucleus - FocusOn 098
Targets	Estrogen receptor alpha antibody

Accessory Products

Proteins and/or Positive Controls

Proteins for Estrogen receptor alpha (3 products)

Catalog No.	Species	Pres.	Purity		Source
H00002099-P01	Estrogen receptor alpha
	Human	Purified					2 µg / €250.00
							Abnova Taiwan Corp.
H00002099-Q01-10	Estrogen receptor alpha
	Human	Purified					10 µg / €350.00
							Abnova Taiwan Corp.
H00002099-Q01-25	Estrogen receptor alpha
	Human	Purified					25 µg / €500.00
							Abnova Taiwan Corp.

Positive controls for Estrogen receptor alpha (1 products)

Catalog No.	Species	Pres.	Purity		Source
NBL1-10344	Estrogen Receptor alpha Lysate
							0.1 mg / €500.00
							Novus Biologicals Inc.

Recommended Secondary Antibodies for Estrogen receptor alpha (6 products)

Catalog No.	Host	Clone/Iso.	Pres.		React.	Applications
R1364AP	Rabbit IgG (H+L chain) antibody
	Goat		AP
							Acris Antibodies GmbH
R1364B	Rabbit IgG (H+L chain) antibody
	Goat		Biotin
							Acris Antibodies GmbH
R1364F	Rabbit IgG (H+L chain) antibody
	Goat		FITC
							Acris Antibodies GmbH
R1364HRP	Rabbit IgG (H+L chain) antibody
	Goat		HRP
							Acris Antibodies GmbH
R1364T	Rabbit IgG (H+L chain) antibody
	Goat		TRITC
							Acris Antibodies GmbH
R1427R	Rabbit IgG F(ab)2 Fragment (H+L chain) antibody
	Donkey		PE
							Acris Antibodies GmbH

Click here to see all secondary antibodies for Estrogen receptor alpha

Service

Acris Antibodies GmbH
Schillerstraße 5
D-32052 Herford
Germany
phone: +49-5221-34606-0
free call: 0800-227 47 46 (Germany only)
fax: +49-5221-34606-11
email: info@acris-online.de
orders: ordering@acris-online.de

© Copyright 1998 - 2013 Acris Antibodies GmbH