Estrogen receptor alpha pSer118 - AP02370PU-N | acris-antibodies.com

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Product Description for Estrogen receptor alpha

Rabbit anti Human, Mouse Estrogen receptor alpha.
Properties: pSer118
Presentation: Aff - Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot, Paraffin Sections.

Properties for Estrogen receptor alpha

Product Category	Antibodies
Quantity	0.1 mg
Synonyms	ER alpha, ESR, ESR1, Estradiol receptor, FocusOn050, FocusOn098, NR3A1, Nuclear receptor subfamily 3 group A member 1
Presentation	Aff - Purified
Reactivity	Hu, Ms
Applications	ICC/IF, P, WB
Clonality	Polyclonal
Host	Rabbit
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	Acris Antibodies GmbH

Datasheet Extract

Immunogen	Swiss Prot Num: P03372 Immunogen: Synthetic phosphopeptide derived from human Estrogen Receptor-alpha around the phosphorylation site of serine 118 (Q-L-SP-P-F). Genename: 2099
Property	pSer118
Antibody type	Ab
Application	Western blot: 1/500 - 1/1000; Incubate membrane with diluted antibody in 5% nonfat milk, 1X TBS, 0,1% Tween-20 at 4°C with gentle shaking, overnight. Immunohistochemistry on paraffin sections: 1/50 - 1/100. Immunofluorescence: 1/100 - 1/200.
Background	Estrogen Receptor alpha is a 65 kDa protein and a member of the steroid family of nuclear receptors. Estrogen Receptor alpha is a ligand-activated transcription factor that, when bound to estrogen, induces a conformational change that allows dimerization and binding to estrogen response element sequences. When bound to DNA, Estrogen Receptor alpha can positively or negatively regulate gene transcription. Like other steroid hormone receptors, Estrogen Receptors are intracellular proteins. Estrogen Receptors play an important role in regulating mammary gland growth and differentiation. Breast tumors that are ER positive may also show a more favorable response to anti-estrogen therapies.
Concentration	1.0 mg/ml
General Readings	Medunjanin S, et al. (2005). J Biol Chem.80 (38):33006-33014. Masuhiro Y, et al. (2005). Proc Natl Acad Sci U S A.102 (23): 8126-8131. Pentecost BT, et al. (2005). Mol Cell Endocrinol.238 (1-2): 9-25. Park KJ, et al. (2005). Mol Cell.18 (1): 71-82.
Storage	Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing. Shelf life: One year from despatch.
Format	Purification: Immunoaffinity chromatography Buffer System: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl containing 0.02% Sodium Azide as preservative and 50% Glycerol as stabilizer State: Liquid purified Ig fraction Aff - Purified
Specificity	Specificity: Estrogen Receptor-alpha (phospho-Ser118) antibody detects endogenous levels of Estrogen Receptor-alpha only when phosphorylated at serine 118. Species: Human and Mouse.
Gene ID	2099
FocusOn and Reviews	FocusOns: Antibodies to Estrogen Receptors - FocusOn 050
Targets	Estrogen receptor alpha antibody

Accessory Products

Proteins and/or Positive Controls

Proteins for Estrogen receptor alpha (3 products)

Catalog No.	Species	Pres.	Purity		Source
H00002099-P01	Estrogen receptor alpha
	Human	Purified					2 µg / €250.00
							Abnova Taiwan Corp.
H00002099-Q01-10	Estrogen receptor alpha
	Human	Purified					10 µg / €350.00
							Abnova Taiwan Corp.
H00002099-Q01-25	Estrogen receptor alpha
	Human	Purified					25 µg / €500.00
							Abnova Taiwan Corp.

Positive controls for Estrogen receptor alpha (1 products)

Catalog No.	Species	Pres.	Purity		Source
NBL1-10344	Estrogen Receptor alpha Lysate
							0.1 mg / €500.00
							Novus Biologicals Inc.

Recommended Secondary Antibodies for Estrogen receptor alpha (6 products)

Catalog No.	Host	Clone/Iso.	Pres.		React.	Applications
R1364AP	Rabbit IgG (H+L chain) antibody
	Goat		AP
							Acris Antibodies GmbH
R1364B	Rabbit IgG (H+L chain) antibody
	Goat		Biotin
							Acris Antibodies GmbH
R1364F	Rabbit IgG (H+L chain) antibody
	Goat		FITC
							Acris Antibodies GmbH
R1364HRP	Rabbit IgG (H+L chain) antibody
	Goat		HRP
							Acris Antibodies GmbH
R1364T	Rabbit IgG (H+L chain) antibody
	Goat		TRITC
							Acris Antibodies GmbH
R1427R	Rabbit IgG F(ab)2 Fragment (H+L chain) antibody
	Donkey		PE
							Acris Antibodies GmbH

Click here to see all secondary antibodies for Estrogen receptor alpha

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