Estrogen receptor beta (Isoform 1) (C-term), 10 x conc. - SM1682X

Product Description for Estrogen receptor beta

Mouse anti Human Estrogen receptor beta PPG5/10.
Properties: (Isoform 1) (C-term), 10 x conc.
Presentation: Supernatant
Product is tested for Immunocytochemistry/Immunofluorescence, Frozen Sections, Paraffin Sections.

Properties for Estrogen receptor beta

Product Category	Antibodies
Quantity	1 ml
Synonyms	ER-beta, ESR2, ESTRB, FocusOn050, FocusOn098, NR3A2, Nuclear receptor subfamily 3 group A member 2
Presentation	Supernatant
Reactivity	Hu
Applications	C, ICC/IF, P
Clonality	Monoclonal
Clone	PPG5/10
Host	Mouse
Isotype	IgG2a
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	Acris Antibodies GmbH

Datasheet Extract

Immunogen	Swiss Prot Num: Q92731 Immunogen: Synthetic peptide derived from the C-terminus of the Human oestrogen receptor beta 1 isoform. Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line. AA Sequence: `CEDSKSKEGSQNPQSQ` Genename: 2100
Property	10 x conc., C-term, Isoform 1
Antibody type	Ab
Application	Immunofluorescence. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections: 1/5-1/50. This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Recommended Positive Control: Ovary. Clone PPG5/10 has also been reported to be suitable for Western blotting.
Background	Estrogen Receptor beta is a recent discovery, initially cloned and characterized from testis. Estrogen Receptor beta is highly homologous to Estrogen Receptor alpha; the ligand and DNA binding domains are highly conserved, while the amino terminus (transactivation domain), has diverged significantly. Estrogen Receptor beta binds estrogen with high affinity and regulates estrogen specific gene activation through direct interaction with estrogen response elements (EREs).
General Readings	Moore, J. et al. (1998) Cloning and characterisation of human oestrogen receptor beta isoforms. Biochem. Biophys. Res. Comm. 247: 75-78. Saunders PT, Millar MR, Williams K, Macpherson S, Harkiss D, Anderson RA, et al. Differential expression of estrogen receptor-alpha and -beta and androgen receptor in the ovaries of marmosets and humans. Biol Reprod. 2000 Oct;63(4):1098-105. PubMed PMID: 10993832. Torlakovic E, Lilleby W, Torlakovic G, Fosså SD, Chibbar R. Prostate carcinoma expression of estrogen receptor-beta as detected by PPG5/10 antibody has positive association with primary Gleason grade and Gleason score. Hum Pathol. 2002 Jun;33(6):646-51. PubMed PMID: 12152165. Saunders, P.T.K. et al. (2002) Expression of oestrogen receptor beta (ERbeta 1) protein in human breast cancer biopsies. Br. J. Cancer. 86: 250-256. Wong. N. A. C. S. et al. (2005) ER beta isoform expression in colorectal carcinoma: an in vivo and in vitro study of clincopathological and molecular correlates. J. Pathol. 207: 53-60. Fixemer, T. et al. (2003) Differential expression of the estrogen receptor beta (ERbeta) in human prostate tissue, premalignant changes, and in primary, metastatic, and recurrent prostatic adenocarcinoma. Prostate. 54: 79-87. Wood CE, Usborne AL, Starost MF, Tarara RP, Hill LR, Wilkinson LM, et al. Hyperplastic and neoplastic lesions of the mammary gland in macaques. Vet Pathol. 2006 Jul;43(4):471-83. PubMed PMID: 16846989. Juengel JL, Heath DA, Quirke LD, McNatty KP. Oestrogen receptor alpha and beta, androgen receptor and progesterone receptor mRNA and protein localisation within the developing ovary and in small growing follicles of sheep. Reproduction. 2006 Jan;131(1):81-92. PubMed PMID: 16388012. Collins F, MacPherson S, Brown P, Bombail V, Williams AR, Anderson RA, et al. Expression of oestrogen receptors, ERalpha, ERbeta, and ERbeta variants, in endometrial cancers and evidence that prostaglandin F may play a role in regulating expression of ERalpha. BMC Cancer. 2009 Sep 16;9:330. doi: 10.1186/1471-2407-9-330. PubMed PMID: 19758455. Carpino, A. et al. (2007) Detection of aromatase and estrogen receptors (ERalpha, ERbeta1, ERbeta2) in human Leydig cell tumor. Eur. J. Endocrinol. 157: 239-244 Bianchi C, Sahlin L, Meikle A, Masironi B, Cavilla M, Aba M. Endometrial population of oestrogen receptors alpha and beta and progesterone receptors A and B during the different phases of the follicular wave of llamas (Lama glama). Reprod Domest Anim. 2010 Oct;45(5):872-80. doi: 10.1111/j.1439-0531.2009.01401.x. PubMed PMID: 19548916. Marotti JD, Collins LC, Hu R, Tamimi RM. Estrogen receptor-beta expression in invasive breast cancer in relation to molecular phenotype: results from the Nurses' Health Study. Mod Pathol. 2010 Feb;23(2):197-204. doi: 10.1038/modpathol.2009.158. Epub 2009 Nov 6. PubMed PMID: 19898422. Saunders PT, Millar MR, Williams K, Macpherson S, Harkiss D, Anderson RA, et al. Differential expression of estrogen receptor-alpha and -beta and androgen receptor in the ovaries of marmosets and humans. Biol Reprod. 2000 Oct;63(4):1098-105. PubMed PMID: 10993832. Asgari, M. and Morakabati, A. (2011) Estrogen receptor beta expression in prostate adenocarcinoma. Diagn Pathol. 6: 61. Fowler PA, Anderson RA, Saunders PT, Kinnell H, Mason JI, Evans DB, et al. Development of steroid signaling pathways during primordial follicle formation in the human fetal ovary. J Clin Endocrinol Metab. 2011 Jun;96(6):1754-62. doi: 10.1210/jc.2010-2618. Epub 2011 Mar 23. PubMed PMID: 21430025. Mafuvadze B, Benakanakere I, López Pérez FR, Besch-Williford C, Ellersieck MR, Hyder SM. Apigenin prevents development of medroxyprogesterone acetate-accelerated 7,12-dimethylbenz(a)anthracene-induced mammary tumors in Sprague-Dawley rats. Cancer Prev Res (Phila). 2011 Aug;4(8):1316-24. doi: 10.1158/1940-6207.CAPR-10-0382. Epub 2011 Apr 19. PubMed PMID: 21505181. Silvestri A, Fraser HM. Oestrogen and progesterone receptors in the marmoset endometrium: changes during the ovulatory cycle, early pregnancy and after inhibition of vascular endothelial growth factor, GnRH or ovariectomy. Reproduction. 2007 Aug;134(2):341-53. PubMed PMID: 17660243.
Storage	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Format	Preservatives: 0.09% Sodium Azide State: Liquid Tissue Culture Supernatant.This antibody is a 10x concentrate, more suitable for rapid staining procedures than the standard SM1682. Supernatant
Specificity	Specificity: This antibody recognises the Estrogen Receptor beta 1 isoform. The antibody has been used for the Immunohistochemical study of the expression of this isoform, showing positive nuclear staining in cells within the ovary, uterus, oviduct, breast, testis, prostate, bladder and male reproductive ducts. Species: Human, Cynomolgus Monkey, Rhesus Monkey, New World Primate, Primate, Rat, Sheep, Pig, Marmoset, Llama.
Gene ID	2100
FocusOn and Reviews	FocusOns: Organelle Markers: Antibodies to the Nucleus - FocusOn 098
Targets	Estrogen receptor beta antibody

Accessory Products

Proteins and/or Positive Controls

Proteins for Estrogen receptor beta (2 products)

Catalog No.	Species	Source
H00002100-Q01-10	Estrogen receptor beta
	Human	in vitro transl.	10 µg / €350.00
			Abnova Taiwan Corp.
H00002100-Q01-25	Estrogen receptor beta
	Human	in vitro transl.	25 µg / €500.00
			Abnova Taiwan Corp.

Positive controls for Estrogen receptor beta (1 products)

Catalog No.	Species
NBL1-10345	Estrogen Receptor beta Lysate
		0.1 mg / €500.00
		Novus Biologicals Inc.

Recommended Secondary Antibodies for Estrogen receptor beta (8 products)

Catalog No.	Host	Pres.
R1253AP	Mouse IgG (H+L chain) antibody
	Rabbit	AP
			Acris Antibodies GmbH
R1253B	Mouse IgG (H+L chain) antibody
	Rabbit	Biotin
			Acris Antibodies GmbH
R1253F	Mouse IgG (H+L chain) antibody
	Rabbit	FITC
			Acris Antibodies GmbH
R1253HRP	Mouse IgG (H+L chain) antibody
	Rabbit	HRP
			Acris Antibodies GmbH
R1253TR	Mouse IgG (H+L chain) antibody
	Rabbit	Texas Red
			Acris Antibodies GmbH
R1403R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Rabbit	PE
			Acris Antibodies GmbH
R1405R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Goat	PE
			Acris Antibodies GmbH
R1455R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Donkey	PE
			Acris Antibodies GmbH