FBL - AM08249PU-N | acris-antibodies.com

Product Description for FBL

Mouse anti C. elegans, Drosophila, Human, Plant, Rat FBL 38F3.
Presentation: Ig Fraction
Product is tested for Frozen Sections, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot.

Properties for FBL

Product Category	Antibodies
Quantity	0.1 ml
Synonyms	FIB1, FLRN, Fibrillarin, FocusOn098, LOT3, NOP1, Nucleolar Marker, Nucleolar protein 1, RNU3IP1, U3 small nucleolar RNA-associated protein NOP1, rRNA 2'-O-methyltransferase fibrillarin
Presentation	Ig Fraction
Reactivity	Ce, Dros, Hu, Pl, Rt, S. pombe
Applications	C, ICC/IF, WB
Clonality	Monoclonal
Clone	38F3
Host	Mouse
Isotype	IgG1
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	Acris Antibodies GmbH

Datasheet Extract

Immunogen	Swiss Prot Num: P15646 Immunogen: Yeast nuclear preparations. Genename: 851548
Antibody type	Ab
Application	Western Blot: 1/1,000. Immunofluorescence: 1/500. Immunohistochemistry.
Background	Nop1p was originally identified as a nucleolar protein of bakers yeast, Saccharomyces cerevisiae. The Nop1p protein is 327 amino acids in size (34.5kDa), is essential for yeast viability, and is localized in the nucleoli (1). The systematic name for S. cerevisiae Nop1 is YDL014W, and it is now known to be part of the small subunit processome complex, involved in the processing of pre-18S ribosomal RNA. Nop1p is the yeast homologue of a protein found in all eukaryotes and archea generally called fibrillarin (2). Fibrillarin/Nop1p is extraordinarily conserved, so that the yeast and human proteins are 67% identical, and the human protein can functionally replace the yeast protein. Patients with the autoimmune disease scleroderma often have strong circulating autoantibodies to a ~34kDa protein which was subsequently found to be fibrillarin. Recent studies show that knock-out of the fibrillarin gene in mice results in embryonic lethality, although mice with only one functional fibrillarin/Nop1p gene were viable (3).
General Readings	Ochs RL, Lischwe MA, Spohn WH, Busch H. Fibrillarin: a new protein of the nucleolus identified by autoimmune sera. Biol Cell. 1985;54(2):123-33. PubMed PMID: 2933102. Aris JP, Blobel G. Identification and characterization of a yeast nucleolar protein that is similar to a rat liver nucleolar protein. J Cell Biol. 1988 Jul;107(1):17-31. PubMed PMID: 3292539. Newton K, Petfalski E, Tollervey D, Cáceres JF. Fibrillarin is essential for early development and required for accumulation of an intron-encoded small nucleolar RNA in the mouse. Mol Cell Biol. 2003 Dec;23(23):8519-27. PubMed PMID: 14612397. Tyagi S, Alsmadi O. Imaging native beta-actin mRNA in motile fibroblasts. Biophys J. 2004 Dec;87(6):4153-62. Epub 2004 Sep 17. PubMed PMID: 15377515. Paeschke1 K, Simonsson T, Postberg J, Rhodes D, Lipps H-J. Telomere end-binding proteins control the formation of G-quadruplex DNA structures in vivo Nature Structural & Molecular Biology 12, 847-854 (2005). Vermaak D, Henikoff S, Malik HS. Positive selection drives the evolution of rhino, a member of the heterochromatin protein 1 family in Drosophila. PLoS Genet. 2005 Jul;1(1):96-108. Epub 2005 Jul 25. PubMed PMID: 16103923.
Storage	Store the antibody undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Format	State: Total IgG fraction containing 10 mM Sodium Azide as preservative. Ig Fraction
Species Reactivity	Species reactivity (expected): Mammals. Species reactivity (tested): Human, Rat, Plant, Drosophila, C. elegans and S. pombe.
Specificity	Specificity: Specific for the ~34kDa Fibrillarin /Nop1p protein. This antibody is becoming widely used as a convenient marker for nucleoli in a wide variety of species (e.g. 4-6).
Gene ID	851548
FocusOn and Reviews	FocusOns: Organelle Markers: Antibodies to the Nucleus - FocusOn 098
Targets	FBL antibody

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