Fibroblasts (Pan Reticular) - BM4018

Product Description for Fibroblasts (Pan Reticular)

Rat anti Human, Mouse, Porcine Fibroblasts (Pan Reticular) ER-TR7.
Presentation: Purified
Product is tested for Frozen Sections, Immunocytochemistry/Immunofluorescence.

Properties for Fibroblasts (Pan Reticular)

Product Category	Antibodies
Quantity	0.2 mg
Synonyms	Fibroblast Marker, Fibroblasten, FocusOn008
Presentation	Purified
Reactivity	Hu, Ms, Por
Applications	C, ICC/IF
Clonality	Monoclonal
Clone	ER-TR7
Host	Rat
Isotype	IgG2a
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	Acris Antibodies GmbH
Material safety datasheet	MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen	Immunogen: Murine thymic reticulum. Epitope: The antigen has not been fully characterized. The epitope may be part of reticulin.
Antibody type	Ab
Application	Immunohistochemistry on Frozen Sections: 1 µg/ml (1/400) freshly prepared for Mouse tissue and 4 µg/ml (1/100) on Human/Porcine tissue. Recommended Positive Control: Mouse spleen. Does not react on routinely processed Paraffin Sections. Has been described to work in FACS.
Background	Connective tissue consists of a ground of glycosaminoglycans, proteoglycans and glycoproteins through which various fibres run. These fibres can be collagenous, elastic or reticular. Reticular fibres are composed from the family of collagen proteins and give tensile strength. These fibres are made by reticular fibroblasts.
Concentration	0.4 mg/ml (after reconstitution)
Protocols	Protocol with frozen, ice-cold acetone-fixed sections: (The whole procedure is performed at room temperature) 1. Wash in PBS 2. Block endogenous peroxidase 3. Wash in PBS 4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber 5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber 6. Wash in PBS 7. Incubate with secondary antibody (peroxidase-conjugated goat anti rat IgG (H+L) minimal-cross reaction to mouse) for 1h in a humid chamber 8. Wash in PBS 9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS 11. Counterstain with Mayers hemalum
Product Citations	Purchased from Acris: Choi BK, Kim YH, Kwon PM, Lee SC, Kang SW, Kim MS, et al. 4-1BB functions as a survival factor in dendritic cells. J Immunol. 2009 Apr 1;182(7):4107-15. doi: 10.4049/jimmunol.0800459. PubMed PMID: 19299708. Malan D, Reppel M, Dobrowolski R, Roell W, Smyth N, Hescheler J, et al. Lack of laminin gamma1 in embryonic stem cell-derived cardiomyocytes causes inhomogeneous electrical spreading despite intact differentiation and function. Stem Cells. 2009 Jan;27(1):88-99. doi: 10.1634/stemcells.2008-0335. PubMed PMID: 18927478. Achtman AH, Höpken UE, Bernert C, Lipp M. CCR7-deficient mice develop atypically persistent germinal centers in response to thymus-independent type 2 antigens. J Leukoc Biol. 2009 Mar;85(3):409-17. doi: 10.1189/jlb.0308162. Epub 2008 Dec 12. PubMed PMID: 19074554. Rangel-Moreno J, Moyron-Quiroz JE, Hartson L, Kusser K, Randall TD. Pulmonary expression of CXC chemokine ligand 13, CC chemokine ligand 19, and CC chemokine ligand 21 is essential for local immunity to influenza. Proc Natl Acad Sci U S A. 2007 Jun 19;104(25):10577-82. Epub 2007 Jun 11. PubMed PMID: 17563386. Rangel-Moreno J, Moyron-Quiroz J, Kusser K, Hartson L, Nakano H, Randall TD. Role of CXC chemokine ligand 13, CC chemokine ligand (CCL) 19, and CCL21 in the organization and function of nasal-associated lymphoid tissue. J Immunol. 2005 Oct 15;175(8):4904-13. PubMed PMID: 16210592. Bajénoff M, Glaichenhaus N, Germain RN. Fibroblastic reticular cells guide T lymphocyte entry into and migration within the splenic T cell zone. J Immunol. 2008 Sep 15;181(6):3947-54. PubMed PMID: 18768849. Bajénoff M, Germain RN. B-cell follicle development remodels the conduit system and allows soluble antigen delivery to follicular dendritic cells. Blood. 2009 Dec 3;114(24):4989-97. doi: 10.1182/blood-2009-06-229567. Epub 2009 Aug 27. PubMed PMID: 19713459. Rehm A, Mensen A, Schradi K, Gerlach K, Wittstock S, Winter S, et al. Cooperative function of CCR7 and lymphotoxin in the formation of a lymphoma-permissive niche within murine secondary lymphoid organs. Blood. 2011 Jul 28;118(4):1020-33. doi: 10.1182/blood-2010-11-321265. Epub 2011 May 17. PubMed PMID: 21586747. Mionnet C, Sanos SL, Mondor I, Jorquera A, Laugier JP, Germain RN, et al. High endothelial venules as traffic control points maintaining lymphocyte population homeostasis in lymph nodes. Blood. 2011 Dec 1;118(23):6115-22. doi: 10.1182/blood-2011-07-367409. Epub 2011 Sep 21. PubMed PMID: 21937697. Rode I, Boehm T. Regenerative capacity of adult cortical thymic epithelial cells. Proc Natl Acad Sci U S A. 2012 Feb 28;109(9):3463-8. doi: 10.1073/pnas.1118823109. Epub 2012 Feb 13. PubMed PMID: 22331880. Tan KW, Yeo KP, Wong FH, Lim HY, Khoo KL, Abastado JP, et al. Expansion of cortical and medullary sinuses restrains lymph node hypertrophy during prolonged inflammation. J Immunol. 2012 Apr 15;188(8):4065-80. doi: 10.4049/jimmunol.1101854. Epub 2012 Mar 19. PubMed PMID: 22430738.
General Readings	Van Vliet E, Melis M, Foidart JM, Van Ewijk W. Reticular fibroblasts in peripheral lymphoid organs identified by a monoclonal antibody. J Histochem Cytochem. 1986 Jul;34(7):883-90. PubMed PMID: 3519751.
Storage	Store the lyophilized antibody at 2-8°C. Freeze stock solution and aliquots thereof at -20°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Format	Purification: Affinity Chromatography Buffer System: Stock solution contains PBS, pH 7.2 with 5 mg/ml BSA as a stabilizer and 0.09% Sodium Azide as preservative State: Lyophilized purified IgG fraction Purified Reconstitution: Restore with 0.5 ml distilled water.
Species Reactivity	Species reactivity (tested): Mouse (Reticular Fibroblasts, Reticular Fibres), Pig and Human.
Specificity	Specificity: Reacts with Mouse reticular fibroblasts, reticular fibres and Human tonsil. ER-TR7 detects an antigen present in and produced by reticular fibroblasts. The recognized antigen is most likely distinct from Laminin, Fibronectin, Collagen types I-IV, Heparan Sulfate Proteoglycan, Entactin, and Nidogen. The antibody is useful to stain the microanatomy of various organs, in particular the connective tissue framework in lymphoid organs. The antibody also stains subendothelial deposits in the plaque area of atherosclerotic plaques. Antigen Distribution Isolated Cells: The antigen is found in the cytoplasm of fibroblast cell lines. Tissue Sections: The antigen is expressed in connective tissues which form a supporting network between parenchymal cells of all organs tested so far (see table below). Thus, the supportive mesenchymal structures of larger vessels can be studied. In spleen, a very clear delineation of red and white pulp is obtained. Capsule, sinuses, follicules, paracortex and medullary cords are also clearly delineated in lymph nodes.
FocusOn and Reviews	FocusOns: Fibroblast Marker Antibodies - FocusOn 008

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