Product Description for GFP
Rabbit anti A. victoria GFP.
Properties: (Ads. to Hu, Ms, Rt Serum Proteins)
Presentation: HRP
Product is tested for Frozen Sections, Enzyme Immunoassay, Western blot / Immunoblot.
Properties: (Ads. to Hu, Ms, Rt Serum Proteins)
Presentation: HRP
Product is tested for Frozen Sections, Enzyme Immunoassay, Western blot / Immunoblot.
Properties for GFP
| Product Category | Antibodies |
|---|---|
| Quantity | 0.1 mg |
| Synonyms | FocusOn118, GFP-Tag, Green fluorescent protein |
| Presentation | HRP |
| Reactivity | A. victoria |
| Applications | C, E, WB |
| Clonality | Polyclonal |
| Host | Rabbit |
| Isotype | IgG |
| Shipping to | Worldwide |
| PDF datasheet | View Datasheet |
| Manufacturer | Acris Antibodies GmbH |
Datasheet Extract
| Immunogen |
|
|---|---|
| Property | Ads. to Hu, Ms, Rt Serum Proteins |
| Antibody type | Ab |
| Application | ELISA: 1/15,000-1/60,000. Western blot: 1/1,000-1/5,000. Immunohistochemistry on Frozen Sections: 1/500-1/3,000. |
| Background | Green fluorescence protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology. YFP differs from GFP due to a mutation at T203Y; antibodies raised against full-length GFP should also detect YFP and other variants. |
| Concentration | 1.0 mg/ml (by UV absorbance at 280 nm) |
| Storage | Store vial at 2-8°C prior to restoration. Following restoration product can be stored undiluted at 2-8°C for one month or (in aliquots) at -20°C or below. Avoid repeated freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Shelf life: One year from despatch. |
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| Species Reactivity |
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| Specificity |
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| Targets |
Accessory Products
Proteins and/or Positive Controls
Proteins for GFP (2 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| AR09180PU-L |
GFP (1-238) |
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A. victoria | Purified | > 95 % | E. coli |
0.5 mg /
€680.00
|
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| Acris Antibodies GmbH | |||||||
| AR09180PU-N |
GFP (1-238) |
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A. victoria | Purified | > 95 % | E. coli |
0.1 mg /
€260.00
|
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| Acris Antibodies GmbH | |||||||
Recommended Secondary Antibodies for GFP (6 products)
| Catalog No. | Host | Clone/Iso. | Pres. | React. | Applications | ||
|---|---|---|---|---|---|---|---|
| R1364AP |
Rabbit IgG (H+L chain) antibody |
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|
Goat | AP | |||||
| Acris Antibodies GmbH | |||||||
| R1364B |
Rabbit IgG (H+L chain) antibody |
||||||
|
Goat | Biotin | |||||
| Acris Antibodies GmbH | |||||||
| R1364F |
Rabbit IgG (H+L chain) antibody |
||||||
|
Goat | FITC | |||||
| Acris Antibodies GmbH | |||||||
| R1364HRP |
Rabbit IgG (H+L chain) antibody |
||||||
|
Goat | HRP | |||||
| Acris Antibodies GmbH | |||||||
| R1364T |
Rabbit IgG (H+L chain) antibody |
||||||
|
Goat | TRITC | |||||
| Acris Antibodies GmbH | |||||||
| R1427R |
Rabbit IgG F(ab)2 Fragment (H+L chain) antibody |
||||||
|
Donkey | PE | |||||
| Acris Antibodies GmbH | |||||||






