Product Description for GFP
Chicken anti A. victoria GFP.
Properties: (Ads. to Hu, Ms, Rt Serum Proteins)
Presentation: Aff - Purified
Product is tested for Enzyme Immunoassay, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot.
Properties: (Ads. to Hu, Ms, Rt Serum Proteins)
Presentation: Aff - Purified
Product is tested for Enzyme Immunoassay, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot.
Properties for GFP
| Product Category | Antibodies |
|---|---|
| Quantity | 0.1 mg |
| Synonyms | FocusOn118, GFP-Tag, Green fluorescent protein |
| Presentation | Aff - Purified |
| Reactivity | A. victoria |
| Applications | E, ICC/IF, WB |
| Clonality | Polyclonal |
| Host | Chicken |
| Isotype | Ig |
| Shipping to | Worldwide |
| PDF datasheet | View Datasheet |
| Manufacturer | Acris Antibodies GmbH |
Datasheet Extract
| Immunogen |
|
|---|---|
| Property | Ads. to Hu, Ms, Rt Serum Proteins |
| Antibody type | Ab |
| Application | ELISA: 1/11,000 - 1/12,000. Western Blot: 1/300 - 1/350. |
| Background | Green fluorescence protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology. YFP differs from GFP due to a mutation at T203Y; antibodies raised against full-length GFP should also detect YFP and other variants. |
| Concentration | 0.64 mg/ml (by UV absorbance at 280 nm) |
| Storage | Store the antibody at -20°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch. Storage Conditions for Trial Size: This vial contains a relatively low volume of reagent (25 µl). To minimize loss of volume dilute 1:10 by adding 225 µl of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Shelf life: 3 month from despatch. |
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Accessory Products
Proteins and/or Positive Controls
Proteins for GFP (2 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| AR09180PU-L |
GFP (1-238) |
||||||
 |
A. victoria | Purified | > 95 % | E. coli |
0.5 mg /
€680.00
|
||
| Acris Antibodies GmbH | |||||||
| AR09180PU-N |
GFP (1-238) |
||||||
|
A. victoria | Purified | > 95 % | E. coli |
0.1 mg /
€260.00
|
||
| Acris Antibodies GmbH | |||||||
Recommended Secondary Antibodies for GFP (5 products)
| Catalog No. | Host | Clone/Iso. | Pres. | React. | Applications | ||
|---|---|---|---|---|---|---|---|
| R1300AP |
Chicken IgG / Chicken IgY (H+L chain) antibody |
||||||
|
Rabbit | AP | |||||
| Acris Antibodies GmbH | |||||||
| R1300B |
Chicken IgG / Chicken IgY (H+L chain) antibody |
||||||
|
|
Rabbit | Biotin | |||||
| Acris Antibodies GmbH | |||||||
| R1300F |
Chicken IgG / Chicken IgY (H+L chain) antibody |
||||||
|
|
Rabbit | FITC | |||||
| Acris Antibodies GmbH | |||||||
| R1300HRP |
Chicken IgG / Chicken IgY (H+L chain) antibody |
||||||
|
|
Rabbit | HRP | |||||
| Acris Antibodies GmbH | |||||||
| R1300T |
Chicken IgG / Chicken IgY (H+L chain) antibody |
||||||
|
|
Rabbit | TRITC | |||||
| Acris Antibodies GmbH | |||||||
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