Product Description for GFP
Goat anti GFP.
Properties: (Ads. to Hu, Ms, Rt Serum Proteins)
Presentation: HRP
Product is tested for Frozen Sections, Enzyme Immunoassay, Western blot / Immunoblot.
Properties: (Ads. to Hu, Ms, Rt Serum Proteins)
Presentation: HRP
Product is tested for Frozen Sections, Enzyme Immunoassay, Western blot / Immunoblot.
Properties for GFP
| Product Category | Antibodies |
|---|---|
| Quantity | 1 mg |
| Synonyms | FocusOn118, GFP-Tag, Green fluorescent protein |
| Presentation | HRP |
| Applications | C, E, WB |
| Clonality | Polyclonal |
| Host | Goat |
| Shipping to | Worldwide |
| PDF datasheet | View Datasheet |
| Manufacturer | Acris Antibodies GmbH |
Datasheet Extract
| Immunogen |
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|---|---|
| Property | Ads. to Hu, Ms, Rt Serum Proteins |
| Antibody type | Ab |
| Add. information | Do Not Add Sodium Azide. |
| Application | Polyclonal anti-GFP antibody is designed to detect GFP and its variants. This antibody can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen and recognizes wild type, recombinant and enhanced forms of GFP. Biotin conjugated polyclonal anti-GFP antibody used in a sandwich ELISA is well suited to titrate GFP in solution using this antibody in combination with a monoclonal anti-GFP antibody (Cat#R1461P) using either form of the antibody as the capture or detection antibodies. However, use the monoclonal form only for the detection of wild type or recombinant GFP as this form does not sufficiently detect 'enhanced' GFP. The detection antibody is typically conjugated to biotin and subsequently reacted with streptavidin conjugated HRP. Fluorochrome conjugated polyclonal anti-GFP antibody can be used to detect GFP by immunofluorescence microscopy in prokaryotic (E.coli) and eukaryotic (CHO cells) expression systems and can detect GFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated polyclonal anti-GFP antibody relative to the fluorescence of GFP alone. For Immunoblotting use either Alkaline Phosphatase or Peroxidase conjugated polyclonal anti-GFP antibody to detect GFP or GFP containing proteins on western blots. Recommended Dilutions: ELISA: 1/10,000-1/50,000. Western blot: 1/2,000-1/5,000. Immunohistochemistry: 1/500-1/2,000. |
| Background | Green fluorescence protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology. YFP differs from GFP due to a mutation at T203Y; antibodies raised against full-length GFP should also detect YFP and other variants. |
| Concentration | 1.0 mg/ml (by UV absorbance at 280 nm) |
| Product Citations | TRITC conjugated antibody is cited in: 1. Suzuki Y, Mogami H, Ihara H, Urano T. Unique secretory dynamics of tissue plasminogen activator and its modulation by plasminogen activator inhibitor-1 in vascular endothelial cells. Blood. 2009 Jan 8;113(2):470-8. doi: 10.1182/blood-2008-03-144279. Epub 2008 Oct 15. PubMed PMID: 18922856. |
| General Readings |
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| Storage | Store vial at 2-8°C prior to restoration. Restore with deionized water or equivalent; centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8°C as an undiluted liquid. For extended storage aliquot contents and freeze at -20°C or below. Avoid repeated freezing and thawing. Dilute only prior to immediate use. Shelf life: one year from despatch. |
| Format |
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| Specificity |
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| FocusOn and Reviews | |
| Targets |
Accessory Products
Proteins and/or Positive Controls
Proteins for GFP (2 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| AR09180PU-L |
GFP (1-238) |
||||||
 |
A. victoria | Purified | > 95 % | E. coli |
0.5 mg /
€680.00
|
||
| Acris Antibodies GmbH | |||||||
| AR09180PU-N |
GFP (1-238) |
||||||
|
A. victoria | Purified | > 95 % | E. coli |
0.1 mg /
€260.00
|
||
| Acris Antibodies GmbH | |||||||
Recommended Secondary Antibodies for GFP (6 products)
| Catalog No. | Host | Clone/Iso. | Pres. | React. | Applications | ||
|---|---|---|---|---|---|---|---|
| R1317AP |
Goat IgG (H+L chain) antibody |
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|
Rabbit | AP | |||||
| Acris Antibodies GmbH | |||||||
| R1317B |
Goat IgG (H+L chain) antibody |
||||||
|
Rabbit | Biotin | |||||
| Acris Antibodies GmbH | |||||||
| R1317F |
Goat IgG (H+L chain) antibody |
||||||
|
Rabbit | FITC | |||||
| Acris Antibodies GmbH | |||||||
| R1317HRP |
Goat IgG (H+L chain) antibody |
||||||
|
Rabbit | HRP | |||||
| Acris Antibodies GmbH | |||||||
| R1317T |
Goat IgG (H+L chain) antibody |
||||||
|
|
Rabbit | TRITC | |||||
| Acris Antibodies GmbH | |||||||
| R1416R |
Goat IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
||||||
|
|
Donkey | PE | |||||
| Acris Antibodies GmbH | |||||||
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