R1091T GFP antibody

See related secondary antibodies

Search for all "GFP"

1 mg / €340.00
Please visit the appropriate website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Goat anti GFP

R1091T

Product Description for GFP

Goat anti GFP.
Presentation: TRITC
Product is tested for Enzyme Immunoassay, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot.

Properties for GFP

Product Category Antibodies
Target Category
Quantity 1 mg
Synonyms GFP-Tag, Green fluorescent protein
Presentation TRITC
Applications E, ICC/IF, WB
Clonality Polyclonal
Host Goat
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P42212
Immunogen:
GST-Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246 aa) derived from the jellyfish Aequorea victoria
Antibody type Ab
Application ELISA (Sandwich or Capture; ELISA for direct binding recognizes wild type, recombinant and enhanced forms of GFP; for sandwich ELISA titrate GFP in solution using either form of the antibody as the capture or detection antibodies, detection antibody is typically conjugated to to biotin and complexed with streptavidin-HRP).
Western blot (Peroxidase conjugated anti-GFP antibody shows a 42 kDa band).
Immunoflourescence.
Background Green fluorescence protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology.

YFP differs from GFP due to a mutation at T203Y; antibodies raised against full-length GFP should also detect YFP and other variants.
Concentration 1.0 mg/ml (by UV absorbance at 280 nm)
Product Citations
Purchased from Acris:
  1. Suzuki Y, Mogami H, Ihara H, Urano T. Unique secretory dynamics of tissue plasminogen activator and its modulation by plasminogen activator inhibitor-1 in vascular endothelial cells. Blood. 2009 Jan 8;113(2):470-8. doi: 10.1182/blood-2008-03-144279. Epub 2008 Oct 15. PubMed PMID: 18922856.
Unconjugated antibody is cited in:
  1. Niv F, Keiner S, Krishna -, Witte OW, Lie DC, Redecker C. Aberrant neurogenesis after stroke: a retroviral cell labeling study. Stroke. 2012 Sep;43(9):2468-75. doi: 10.1161/STROKEAHA.112.660977. Epub 2012 Jun 26. PubMed PMID: 22738919.
Storage Store vial at 2 - 8 °C prior to reconstitution.
Following reconstitution the product is stable for one month at 2 - 8 °C as an undiluted liquid. Dilute only prior to immediate use.
For extended storage add glycerol to 50 % and then aliquot contents and freeze at -20 °C or below. Avoid repeated freezing and thawing.
Centrifuge product if not completely clear after standing at room temperature.
Shelf life: One year from despatch.
Format
Label:
(Molecular weight 444 daltons)
TRITC
Absorption emission:
550 nm / 570 nm
Molar radio:
2.9 moles TRITC per mole of Goat IgG
Purification:
Immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities
Buffer System:
0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0 as a buffer, 10 mg/ml Bovine Serum Albumin (BSA) IgG and (protease free) as stabilizer, 0.01 % (w/v) Sodium azide as preservative
Purity:
/
State:
Lyophilized Ig fraction
Reconstitution:
Restore with 1.0 ml of deionized water (or equivalent).
Specificity
Specificity:
This antibody detects Fluorescent Protein (GFP).
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, anti-Peroxidase and purified and partially purified Green Fluorescent Protein (Aequorea victoria) Serum. No reaction was observed against Human, Mouse and Rat Serum Proteins.
FocusOn and Reviews
FocusOns:

Accessory Products

Proteins and/or Positive Controls

Proteins for GFP (2 products)

Catalog No. Species Pres. Purity   Source  
AR09180PU-L

GFP (1-238)

GFP, 1-238 aa Aequorea victoria, Recombinant, E.coli: 15% SDS-PAGE (3 µg)
A. victoria Purified > 95 % by SDS - PAGE E. coli
0.5 mg / €680.00
  Acris Antibodies GmbH
AR09180PU-N

GFP (1-238)

GFP, 1-238 aa Aequorea victoria, Recombinant, E.coli: 15% SDS-PAGE (3 µg)
A. victoria Purified > 95 % by SDS - PAGE E. coli
0.1 mg / €260.00
  Acris Antibodies GmbH
  • Pinterest
  • LinkedIn