Product Description for GFP
Goat anti GFP.
Presentation: TRITC
Product is tested for Enzyme Immunoassay, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot.
Presentation: TRITC
Product is tested for Enzyme Immunoassay, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot.
Properties for GFP
| Product Category | Antibodies |
|---|---|
| Quantity | 1 mg |
| Synonyms | FocusOn118, GFP-Tag, Green fluorescent protein |
| Presentation | TRITC |
| Applications | E, ICC/IF, WB |
| Clonality | Polyclonal |
| Host | Goat |
| Shipping to | Worldwide |
| PDF datasheet | View Datasheet |
| Manufacturer | Acris Antibodies GmbH |
| Material safety datasheet | MSDS for Polyclonal Antibodies (de) |
Datasheet Extract
| Immunogen |
|
|---|---|
| Antibody type | Ab |
| Application | ELISA (Sandwich or Capture; ELISA for direct binding recognizes wild type, recombinant and enhanced forms of GFP; for sandwich ELISA titrate GFP in solution using either form of the antibody as the capture or detection antibodies, detection antibody is typically conjugated to to biotin and complexed with streptavidin-HRP). Western blot (Peroxidase conjugated anti-GFP antibody shows a 42 kDa band). Immunoflourescence. |
| Background | Green fluorescence protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology. YFP differs from GFP due to a mutation at T203Y; antibodies raised against full-length GFP should also detect YFP and other variants. |
| Concentration | 1.0 mg/ml (by UV absorbance at 280 nm) |
| Product Citations |
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| Storage | Store vial at 2 - 8 °C prior to reconstitution. Following reconstitution the product is stable for one month at 2 - 8 °C as an undiluted liquid. Dilute only prior to immediate use. For extended storage add glycerol to 50 % and then aliquot contents and freeze at -20 °C or below. Avoid repeated freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Shelf life: One year from despatch. |
| Format |
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| Specificity |
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| FocusOn and Reviews | |
| Targets |
Accessory Products
Proteins and/or Positive Controls
Proteins for GFP (2 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| AR09180PU-L |
GFP (1-238) |
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 |
A. victoria | Purified | > 95 % | E. coli |
0.5 mg /
€680.00
|
||
| Acris Antibodies GmbH | |||||||
| AR09180PU-N |
GFP (1-238) |
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|
A. victoria | Purified | > 95 % | E. coli |
0.1 mg /
€260.00
|
||
| Acris Antibodies GmbH | |||||||
Recommended Secondary Antibodies for GFP (6 products)
| Catalog No. | Host | Clone/Iso. | Pres. | React. | Applications | ||
|---|---|---|---|---|---|---|---|
| R1317AP |
Goat IgG (H+L chain) antibody |
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|
Rabbit | AP | |||||
| Acris Antibodies GmbH | |||||||
| R1317B |
Goat IgG (H+L chain) antibody |
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|
Rabbit | Biotin | |||||
| Acris Antibodies GmbH | |||||||
| R1317F |
Goat IgG (H+L chain) antibody |
||||||
|
Rabbit | FITC | |||||
| Acris Antibodies GmbH | |||||||
| R1317HRP |
Goat IgG (H+L chain) antibody |
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|
Rabbit | HRP | |||||
| Acris Antibodies GmbH | |||||||
| R1317T |
Goat IgG (H+L chain) antibody |
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|
|
Rabbit | TRITC | |||||
| Acris Antibodies GmbH | |||||||
| R1416R |
Goat IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
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|
|
Donkey | PE | |||||
| Acris Antibodies GmbH | |||||||
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