GOPC / PIST (Center) - AP20061PU-N | acris-antibodies.com

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Product Description for GOPC / PIST

Rabbit anti Human, Mouse GOPC / PIST.
Properties: (Center)
Presentation: Aff - Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Paraffin Sections, Enzyme Immunoassay, Western blot / Immunoblot.

Properties for GOPC / PIST

Product Category	Antibodies
Quantity	0.1 mg
Synonyms	CAL, CFTR-associated ligand, FIG, Fused in glioblastoma, Golgi-associated PDZ and coiled-coil motif-containing protein, PDZ protein interacting specifically with TC10
Presentation	Aff - Purified
Reactivity	Hu, Ms
Applications	E, ICC/IF, P, WB
Clonality	Polyclonal
Host	Rabbit
Isotype	IgG
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	Acris Antibodies GmbH

Datasheet Extract

Immunogen	Swiss Prot Num: Q9HD26 Immunogen: 17 amino acid peptide from near the center of Human PIST (Genbank accession No. AAG00572). Genename: 57120
Property	Center
Antibody type	Ab
Control peptide	AP20061CP-N
Application	ELISA. Western Blot: PIST antibody can be used for the detection of PIST at 1–2 μg/ml. Positive Control: PC-3 Cell Lysate. Immunofluorescence: Start at 20 μg/ml. Immunohistochemistry on Paraffin Setions. Positive Control: Rat Colon Cell Lysate.
Background	Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components (1,2) and is negatively regulated by TOR (Target of rapamycin) (3). PIST, a PDZ-containing protein, was discovered in a yeast two-hybrid system as a binding partner to Beclin-1, a Bcl-2-interacting protein homologous to the yeast autophagy gene apg6 (4-6). Experiments with mutant PIST proteins lacking the PDZ domain showed that PIST interaction with Beclin-1 through its coiled-coil domain can modulate Beclin-1 activity and suggest that PIST interactions with other proteins through its PDZ domain may regulate the activity of PIST and Beclin-1. (6)
General Readings	Gozuacik D, Kimchi A. Autophagy as a cell death and tumor suppressor mechanism. Oncogene. 2004 Apr 12;23(16):2891-906. PubMed PMID: 15077152. Kisen GO, Tessitore L, Costelli P, Gordon PB, Schwarze PE, Baccino FM, et al. Reduced autophagic activity in primary rat hepatocellular carcinoma and ascites hepatoma cells. Carcinogenesis. 1993 Dec;14(12):2501-5. PubMed PMID: 8269618. Kamada Y, Funakoshi T, Shintani T, et al. Tor-mediated induction of autophagy via Apg1 protein kinase complex. J. Cell. Biol. 2000; 150:1507-13. Yue Z, Horton A, Bravin M, DeJager PL, Selimi F, Heintz N. A novel protein complex linking the delta 2 glutamate receptor and autophagy: implications for neurodegeneration in lurcher mice. Neuron. 2002 Aug 29;35(5):921-33. PubMed PMID: 12372286. Liang XH, Kleeman LK, Jiang HH, Gordon G, Goldman JE, Berry G, et al. Protection against fatal Sindbis virus encephalitis by beclin, a novel Bcl-2-interacting protein. J Virol. 1998 Nov;72(11):8586-96. PubMed PMID: 9765397. Kametaka S, Okano T, Ohsumi M, Ohsumi Y. Apg14p and Apg6/Vps30p form a protein complex essential for autophagy in the yeast, Saccharomyces cerevisiae. J Biol Chem. 1998 Aug 28;273(35):22284-91. PubMed PMID: 9712845.
Storage	Store the antibody undiluted at 2-8°C. Antibodies should not be exposed to prolonged high temperatures. Shelf life: one year from despatch.
Format	Purification: Immunoaffinity Chromatography Buffer System: PBS containing 0.02% Sodium Azide as preservative State: Liquid purified IgG fraction Aff - Purified
Species Reactivity	Species reactivity (tested): Human and Mouse.
Specificity	Specificity: Recognizes PIST (Intermediate Domain).
Gene ID	57120
FocusOn and Reviews	FocusOns: Autophagy - Recycling as a Survival Factor - FocusOn128
Targets	GOPC / PIST antibody

Accessory Products

Proteins and/or Positive Controls

Proteins for GOPC / PIST (3 products)

Catalog No.	Species	Pres.	Purity		Source
AR09384PU-L	GOPC / PIST (278-454, His-tag)
	Human	Purified	> 90 %		E. coli		0.5 mg / €670.00
							Acris Antibodies GmbH
AR09384PU-N	GOPC / PIST (278-454, His-tag)
	Human	Purified	> 90 %		E. coli		0.1 mg / €250.00
							Acris Antibodies GmbH
NBP1-40404	GOPC / PIST
		Liquid					0.1 mg / €310.00
							Novus Biologicals Inc.

Recommended Secondary Antibodies for GOPC / PIST (6 products)

Catalog No.	Host	Clone/Iso.	Pres.		React.	Applications
R1364AP	Rabbit IgG (H+L chain) antibody
	Goat		AP
							Acris Antibodies GmbH
R1364B	Rabbit IgG (H+L chain) antibody
	Goat		Biotin
							Acris Antibodies GmbH
R1364F	Rabbit IgG (H+L chain) antibody
	Goat		FITC
							Acris Antibodies GmbH
R1364HRP	Rabbit IgG (H+L chain) antibody
	Goat		HRP
							Acris Antibodies GmbH
R1364T	Rabbit IgG (H+L chain) antibody
	Goat		TRITC
							Acris Antibodies GmbH
R1427R	Rabbit IgG F(ab)2 Fragment (H+L chain) antibody
	Donkey		PE
							Acris Antibodies GmbH

Click here to see all secondary antibodies for GOPC / PIST

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