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AM03139FC-N Heat shock protein 70 / HSP70 antibody

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50 µg / €260.00
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Mouse anti Bovine, Canine, Carp, Chicken, Drosophila, Guinea Pig, Hamster, Human, Monkey, Mouse, Porcine, Rabbit, Rat, Sheep Heat shock protein 70 / HSP70 C92F3A-5

AM03139FC-N

Product Description for Heat shock protein 70 / HSP70

Mouse anti Bovine, Canine, Carp, Chicken, Drosophila, Guinea Pig, Hamster, Human, Monkey, Mouse, Porcine, Rabbit, Rat, Sheep Heat shock protein 70 / HSP70 C92F3A-5.
Presentation: FITC
Product is tested for Flow Cytometry.

Properties for Heat shock protein 70 / HSP70

Product Category Antibodies
Quantity 50 µg
Synonyms HSP70-1/HSP70-2, HSP70.1, HSPA1, HSPA1A, HSPA1B, Heat shock 70 kDa protein 1A/1B, Review 003
Presentation FITC
Reactivity Bov, Can, Carp, Chk, Dros, GP, Hst, Hu, Mky, Ms, Por, Rb, Rt, Sh
Applications F
Clonality Monoclonal
Clone C92F3A-5
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH

Datasheet Extract

Immunogen
Swiss Prot Num:
P08107
Immunogen:
Human HSP70 (1)
Genename:
3303
Antibody type Ab
Application FACS (12).
Flow Cytometry.
100 µg/ml of this antibody was sufficient for detection of HSP70 in Human Jurkat cells by FACS anaylsis.
Background Hsp70 genes encode abundant heat-inducible 70-kDa hsps (hsp70s). In most eukaryotes hsp70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 5O% identity (2). The N-terminal two thirds of hsp70s are more conserved than the C-terminal third. Hsp70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (3). When hsc70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (4). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins (5). All hsp70s, regardless of location, bind proteins, particularly unfolded ones. The molecular chaperones of the hsp70 family recognize and bind to nascent polypeptide chains as well as partially folded intermediates of proteins preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein (6). The universal ability of hsp70s to undergo cycles of binding to and release from hydrophobic stretches of partially unfolded proteins determines their role in a great variety of vital intracellular functions such as protein synthesis, protein folding and oligomerization and protein transport.
Concentration 0.33 mg/ml
General Readings
  • Welch W.J. and Suhan J.P. (1986) J Cell Biol. 103: 2035-2050. 2. Boorstein W. R., Ziegelhoffer T. & Craig E. A. (1993) J. Mol. Evol. 38(1): 1-17.
  • Rothman J. (1989) Cell 59: 591-601.
  • DeLuca-Flaherty et al. (1990) Cell 62: 875-887.
  • Bork P., Sander C. & Valencia A. (1992) Proc. Nut1 Acad. Sci. USA 89: 7290-7294.
  • Fink A.L. (1999) Physiol. Rev. 79: 425-449.
  • Galan A., et al. (2000) J. Biol. Chem. 275: 11418-11424.
  • Kondo T., et al. (2000) J. Biol. Chem. 275: 8872-8879.
  • Misaki T., et al. (1994) Clin. Exp. Immun. 98: 234-239.
  • Pockley A.G., et al. (1998) Immunol. Invest. 27: 367-377.
  • Moon I.S., et al. (2001) Cereb Cortex 11(3): 238-248.
  • Dressel et al. (2000) J. Immunol. 164: 2362-2371.
Storage Store undiluted at 2-8°C.
This product is photosensitive and should be protected from light.
Shelf life: one year from despatch.
Format
Label:
FITC
Purification:
Affinity Chromatography on Protein G
Buffer System:
PBS, pH 7.2
Preservatives:
0.01% Sodium Azide
Stabilizers:
50% Glycerol
State:
Liquid purified Ig fraction
Specificity
Specificity:
This antibody detects a ~70 kDa protein corresponding to the Molecular Mass of inducible HSP70 on SDS PAGE Immunoblots.
The mapped epitope is in the region of amino acid residues 436-503.
Does not cross-react with HSC70 (HSP73).
Species:
Human, Mouse, Rat, Bovine, Canine, Chicken, Drosophila, Fish (carp), Guinea Pig, Hamster, Monkey, Pig, Rabbit and Sheep.
Gene ID 3303
FocusOn and Reviews
Reviews:
Targets

Accessory Products

Proteins and/or Positive Controls

Proteins for Heat shock protein 70 / HSP70 (15 products)

Catalog No. Species Pres. Purity   Source  
AR03018PU-L

Heat shock protein 70 / HSP70 (active)
Heat shock protein 70 / HSP70 Human Purified > 90 % E. coli
0.2 mg / €710.00
  Acris Antibodies GmbH
AR03018PU-N

Heat shock protein 70 / HSP70 (active)
Heat shock protein 70 / HSP70 Human Purified > 90 % E. coli
0.1 mg / €400.00
  Acris Antibodies GmbH
AR03018PU-S

Heat shock protein 70 / HSP70 (active)
Heat shock protein 70 / HSP70 Human Purified > 90 % E. coli
50 µg / €390.00
  Acris Antibodies GmbH
AR03022PU-L

Heat shock protein 70 / HSP70 (active)
Heat shock protein 70 / HSP70 Human Aff - Purified > 95 % E. coli
0.2 mg / €710.00
  Acris Antibodies GmbH
AR03022PU-N

Heat shock protein 70 / HSP70 (active)
Heat shock protein 70 / HSP70 Human Aff - Purified > 95 % E. coli
0.1 mg / €400.00
  Acris Antibodies GmbH
AR03022PU-S

Heat shock protein 70 / HSP70 (active)
Heat shock protein 70 / HSP70 Human Aff - Purified > 95 % E. coli
50 µg / €390.00
  Acris Antibodies GmbH
AR09035PU-L

Heat shock protein 70 / HSP70 (1-641, His-tag)
HSP70: 15% SDS PAGE Human Purified > 95 % E. coli
0.5 mg / €540.00
  Acris Antibodies GmbH
AR09035PU-N

Heat shock protein 70 / HSP70 (1-641, His-tag)
HSP70: 15% SDS PAGE Human Purified > 95 % E. coli
0.1 mg / €230.00
  Acris Antibodies GmbH
NBC1-18356

Heat shock protein 70 / HSP70
SDS-Page: Hsp70 Protein [NBC1-18356] - Dna K, 17.7kDa (163aa) with a purity of 95% by SDS - PAGE
0.1 mg / €280.00
  Novus Biologicals Inc.
NBC1-18358

Heat shock protein 70 / HSP70
SDS-Page: Hsp70 Protein [NBC1-18358] - Dna K, 41.6kDa (384aa) with a purity of 95% by SDS - PAGE
0.1 mg / €280.00
  Novus Biologicals Inc.
NBC1-18359

Heat shock protein 70 / HSP70
SDS-Page: Hsp70 Protein [NBC1-18359] - Dna K, 27.7kDa (255aa) with a purity of 95% by SDS - PAGE
0.1 mg / €280.00
  Novus Biologicals Inc.
NBC1-18360

Heat shock protein 70 / HSP70
SDS-Page: Hsp70 Protein [NBC1-18360] - Dna K, 14.6kDa (132aa) with a purity of 95% by SDS - PAGE
0.1 mg / €280.00
  Novus Biologicals Inc.
NBC1-18367

Heat shock protein 70 / HSP70
SDS-Page: Hsp70 Protein [NBC1-18367] - HSP70, 72.2kDa (661aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE
0.1 mg / €280.00
  Novus Biologicals Inc.
H00003304-Q01-10

Heat shock protein 70 / HSP70
Heat shock protein 70 / HSP70 Human in vitro transl.
10 µg / €350.00
  Abnova Taiwan Corp.
H00003304-Q01-25

Heat shock protein 70 / HSP70
Heat shock protein 70 / HSP70 Human in vitro transl.
25 µg / €500.00
  Abnova Taiwan Corp.

Positive controls for Heat shock protein 70 / HSP70 (1 products)

Catalog No. Species Pres. Purity   Source  
NBL1-11749

Hsp70 Lysate
Western Blot: Hsp70 Lysate [NBL1-11749] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for HSPA1A
0.1 mg / €370.00
  Novus Biologicals Inc.

Recommended Secondary Antibodies for Heat shock protein 70 / HSP70 (8 products)

Catalog No. Host Clone/Iso. Pres.   React. Applications  
R1253AP

Mouse IgG (H+L chain) antibody
Mouse IgG (H+L chain) antibody Rabbit AP
    Acris Antibodies GmbH
R1253B

Mouse IgG (H+L chain) antibody
Mouse IgG (H+L chain) antibody Rabbit Biotin
    Acris Antibodies GmbH
R1253F

Mouse IgG (H+L chain) antibody
Mouse IgG (H+L chain) antibody Rabbit FITC
    Acris Antibodies GmbH
R1253HRP

Mouse IgG (H+L chain) antibody
Mouse IgG (H+L chain) antibody Rabbit HRP
    Acris Antibodies GmbH
R1253TR

Mouse IgG (H+L chain) antibody
Mouse IgG (H+L chain) antibody Rabbit Texas Red
    Acris Antibodies GmbH
R1403R

Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody Rabbit PE
    Acris Antibodies GmbH
R1405R

Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody Goat PE
    Acris Antibodies GmbH
R1455R

Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody Donkey PE
    Acris Antibodies GmbH
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